{"product_id":"medulloblastoma-9781071619513","title":"Medulloblastoma","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003eThis volume details methods and protocols covering multiple aspects of Medulloblastoma. Divided into four parts, chapters guide readers through nucleic acids detection and analysis, cell-based analysis methodologies, and applications of patient-information on designing better experimental strategies for future drug development efforts in Medulloblastoma. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Medulloblastoma: Methods and Protocols aims to deliver a clear-cut and standardized set of protocols to a broad scientific community.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e  \u003cp\u003e1.     Childhood Medulloblastoma: An Overview\u003c\/p\u003e  \u003cp\u003eYujin Suk, William D Gwynne, Ian Burns, Chitra Venugopal and Sheila K Singh\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  Part I: Molecular Assay-based Methods\u003cp\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e2.     Real-time PCR assays on formalin-fixed paraffin embedded medulloblastomas\u003c\/p\u003e  Ratika Kunder and Neelam Vishwanath Shirsat\u003cp\u003e\u003c\/p\u003e  \u003cp\u003e3.     Validation of microRNA target genes using luciferase reporter assay and western blot analysis\u003c\/p\u003e  \u003cp\u003ePooja Panwalkar, Atul Khire and Neelam Shirsat\u003c\/p\u003e  \u003cp\u003e4.     Chromatin Immunoprecipitation Assays on Medulloblastoma Cell Line DAOY\u003c\/p\u003e  \u003cp\u003eTara Dobson and Jyothishmathi Swaminathan\u003c\/p\u003e  \u003cp\u003e5.     Quantification of microRNAs in cells and tissues using stem-loop RT PCR and qPCR\u003c\/p\u003e  \u003cp\u003ePooja Panwalkar, Atul Khire and Neelam Shirsat\u003c\/p\u003e  \u003cp\u003e6.     \u003ci\u003eAnalysis of Microarray Data from Medulloblastoma tissue samples\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003eDebojyoti Dhar and Gopala Kallapura\u003c\/p\u003e  7.     Advancement of single- cell sequencing in Medulloblastoma \u003cp\u003e\u003c\/p\u003e  \u003cp\u003eDeepanshu Verma, Namyashree Nayak, Ashuthosh Singh, Ashutosh Kumar Singh, Neha Garg\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003ePart II: Cellular Assays and Inhibitors for Medulloblastoma\u003c\/p\u003e  \u003cp\u003e8.     Flow cytometry approaches to obtain medulloblastoma stem cells from bulk cultures\u003c\/p\u003e  \u003cp\u003ePraveen Kumar and Neha Garg\u003c\/p\u003e  9.     Drug Screening assays on Medulloblastoma Stem Cells using compound libraries\u003cp\u003e\u003c\/p\u003e  \u003cp\u003eAshutosh Singh and Neha Garg\u003c\/p\u003e  \u003cp\u003e10.  3D-on-2D: A physiologically-relevant and gel-free \u003ci\u003ein vitro\u003c\/i\u003e co-culture method to assay anti-metastatic agents \u003c\/p\u003e  \u003cp\u003eAbhinav Dey, Samuel Eisenberg, Rayna Birnbaum and David J Sharp\u003c\/p\u003e  \u003cp\u003e11.  In vitro screening assay for activators of T- cell migration to solid tumors\u003c\/p\u003e  \u003cp\u003eAbhinav Dey and David Sharp\u003c\/p\u003e  \u003cp\u003e12.  Impact of Nano preparation of phytoconstituents in Medulloblastoma\u003c\/p\u003e  \u003cp\u003ePriyanka Mishra and Y.B. Tripathi\u003c\/p\u003e  \u003cp\u003e13.  Challenges and Recent Advances of novel chemical inhibitors in Medulloblastoma Therapy\u003c\/p\u003e  \u003cp\u003eAnand Maurya, Upendra Kumar Patel, Jitendra Kumar Yadav, Virender Pratap Singh, Alka Agarwal\u003c\/p\u003e  \u003cp\u003e14.  Role of ayurvedic plants as anti-cancer agents\u003c\/p\u003e  \u003cp\u003eNikhil Pandey and Y.B. Tripathi\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003ePart III: Animal Models in Medulloblastoma \u003c\/p\u003e  \u003cp\u003e15.  \u003ci\u003eIn vivo\u003c\/i\u003e tumorigenicity assays using subcutaneous and orthotopic xenografts in immunodeficient mice\u003c\/p\u003e  \u003cp\u003eKedar Yogi, Rahul Thorat and Neelam Shirsat\u003c\/p\u003e  \u003cp\u003e16.  Manufacture of an Oncolytic Measles Virus-Sensitive Immune-Competent Mouse Model of Medulloblastoma.\u003c\/p\u003e  \u003cp\u003eSangeet Lal and Corey Raffel\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003ePart IV: Patient data based approaches for Biomedical Research in Medulloblastoma\u003c\/p\u003e  17.  Preclinical Research Strategy Development for RNAi-based therapies in Oncology using patient-centred information from public databases of Human Protein Atlas and R2Genomics Platform \u003cp\u003e\u003c\/p\u003e  \u003cp\u003eAbhinav Dey, Isabelle Balachandran, Ava Solis and David J Sharp\u003c\/p\u003e  18.  Magnetic resonance imaging in the contemporary management of medulloblastoma: current and emerging applications  \u003cp\u003e\u003c\/p\u003e  \u003cp\u003eArchya Dasgupta, Madan Maitre,\u003csup\u003e \u003c\/sup\u003eSona Pungavkar and Tejpal Gupta\u003c\/p\u003e  \u003cp\u003e19.  Multidisciplinary Management of Medulloblastoma:  Consensus, Challenges and Controversies \u003c\/p\u003e  \u003cp\u003eAbhishek Chatterjee, Madan Maitre, Archya Dasgupta, Epari Sridhar and Tejpal Gupta\u003c\/p\u003e","brand":"Springer-Verlag New York Inc.","offers":[{"title":"Default Title","offer_id":49406771921239,"sku":"9781071619513","price":134.99,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9781071619513.jpg?v=1730497055","url":"https:\/\/bookcurl.com\/products\/medulloblastoma-9781071619513","provider":"Book Curl","version":"1.0","type":"link"}