{"product_id":"mass-spectrometry-analysis-for-proteinprotein-interactions-and-dynamics-9780470258866","title":"Mass Spectrometry Analysis for ProteinProtein","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003e\u003cul\u003e \u003cli\u003ePresents a wide variety of mass spectrometry methods used to explore structural mechanisms, protein dynamics and interactions between proteins.\u003c\/li\u003e \u003cli\u003ePreliminary chapters cover mass spectrometry methods for examining proteins and are then followed by chapters devoted to presenting very practical, how-to methods in a detailed way.\u003c\/li\u003e \u003cli\u003eIncludes footprinting and plistex specifically, setting this book apart from the competition.\u003c\/li\u003e \u003c\/ul\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003eForeword.  \u003cp\u003eContributors.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1. Overview of Mass Spectrometry Technologies for Examining Protein Structure: Current and Future Directions (Shannon M. Swiatkowski and Mark R. Chance).\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1.1 Introduction.\u003c\/p\u003e \u003cp\u003e1.2 Hydrogen\/Deuterium Exchange Mass Spectrometry.\u003c\/p\u003e \u003cp\u003e1.3 Hydroxyl-Radical-Mediated Protein Footprinting.\u003c\/p\u003e \u003cp\u003e1.4 Chemical Cross-linking.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2. Hydrogen Exchange Mass Spectrometry: Principles and Capabilities (Sebastien Brier and John R. Engen).\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 The Chemistry of Hydrogen Exchange.\u003c\/p\u003e \u003cp\u003e2.2 HX Mechanisms in Proteins.\u003c\/p\u003e \u003cp\u003e2.3 Deuterium Incorporation into Proteins.\u003c\/p\u003e \u003cp\u003e2.4 Measuring HX with Mass Spectrometry.\u003c\/p\u003e \u003cp\u003e2.5 Capabilities of HX MS in Structural Biology.\u003c\/p\u003e \u003cp\u003eAcknowledgment.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3. Covalent Labeling Methods for Examining Protein Structure and Protein Interactions (Keiji Takamoto and Janna Kiselar).\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 Introduction.\u003c\/p\u003e \u003cp\u003e3.2 Chemistry of Hydroxyl Radical Footprinting.\u003c\/p\u003e \u003cp\u003e3.3 Mass Spectrometry Approaches for Quantitative Protein Footprinting.\u003c\/p\u003e \u003cp\u003e3.4 Examples of Various Methods that Generate Hydroxyl Radicals in Solution to Examine Protein Structure.\u003c\/p\u003e \u003cp\u003e3.5 The Future: Hybrid Approaches that Combine Experimental and Computational Data.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4. Complementary Methods for Structure Determination: Hydroxyl-Radical-Mediated Footprinting and Deuterium Exchange Mass Spectrometry as Applied to Serpin Structure (Xiaojing Zheng and Patrick L. Wintrode).\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 Introduction.\u003c\/p\u003e \u003cp\u003e4.2 Technical Comparison of Hydroxyl-Radical-Mediated Footprinting and H\/D Exchange Methodologies.\u003c\/p\u003e \u003cp\u003e4.3 Structural Mass Spectrometry Data.\u003c\/p\u003e \u003cp\u003e4.4 Solvent Accessibility.\u003c\/p\u003e \u003cp\u003e4.5 Dynamics.\u003c\/p\u003e \u003cp\u003e4.6 Significance for Serpin Structure and Function.\u003c\/p\u003e \u003cp\u003e4.7 Summary.\u003c\/p\u003e \u003cp\u003eAcknowledgement.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5. Deuterium Exchange Approaches for Examining Protein Interactions: Case Studies of Complex Formation (Elizabeth A. Komives).\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Interactions of Regulatory and Catalytic Subunits of Protein Kinase A.\u003c\/p\u003e \u003cp\u003e5.2 Allostery in Protein-Protein Interactions Revealed by H\/D Exchange.\u003c\/p\u003e \u003cp\u003e5.3 Interactions of the Inhibitor IkBα with the Transcription Factor NF-kB.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6. Hydrogen\/Deuterium Exchange Studies of Viruses (Sebyung Kang and Peter E. Prevelige Jr.).\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e6.1 Overview of Virus Lifecycles.\u003c\/p\u003e \u003cp\u003e6.2 Structural Investigations of Viral Capsids.\u003c\/p\u003e \u003cp\u003e6.3 Dynamics of Viral Capsids.\u003c\/p\u003e \u003cp\u003e6.4 Hydrogen\/Deuterium Exchange Studies of Virus Capsid Structure.\u003c\/p\u003e \u003cp\u003e6.5 Hydrogen\/Deuterium Exchange Studies of Viral Protein Dyanmics.\u003c\/p\u003e \u003cp\u003e6.6 Technical Aspects of Performing Hydrogen\/Deuterium Exchange Experiments on Viruses.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7. Use of Enhanced Peptide Amide Hydrogen\/Deuterium Exchange-Mass Spectrometry (DXMS) in the Examination of Protein-Protein Interactions (Yoshitomo Hamuro, Stephen J. Coales, Lora L. Hamuro, and Virgil L. Woods Jr.).\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction.\u003c\/p\u003e \u003cp\u003e7.2 Theory of H\/D Exchange.\u003c\/p\u003e \u003cp\u003e7.3 Overview of DXMS Technology for Protein-Protein Interactions.\u003c\/p\u003e \u003cp\u003e7.4 DXMS of Human Growth Hormone and Its Binding Protein.\u003c\/p\u003e \u003cp\u003e7.5 DXMS of PKA Regulatory Subunits.\u003c\/p\u003e \u003cp\u003e7.6 DXMS of PKA R-Submit D\/D Domains and D-AKAP2 AKB Domain.\u003c\/p\u003e \u003cp\u003e7.7 Epitope Mapping by DXMS.\u003c\/p\u003e \u003cp\u003e7.8 Conclusions.\u003c\/p\u003e \u003cp\u003eAbbreviations.\u003c\/p\u003e \u003cp\u003eAcknowledgements.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8. Cross-linking as a Tool to Examine Protein Complexes: Examples of Cross-linking Strategies and Computational Modeling (Evgeniy V. Petrotchenko and Christoph H. Borchers).\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8.1 Introduction.\u003c\/p\u003e \u003cp\u003e8.2 Cross-linking Strategies.\u003c\/p\u003e \u003cp\u003e8.3 Cross-linking Methodology.\u003c\/p\u003e \u003cp\u003e8.4 challenges Associated with Combining Cross-linking with Mass Spectrometry.\u003c\/p\u003e \u003cp\u003e8.5 Advances in Mass Spectrometry Instrumentation and Capabilities.\u003c\/p\u003e \u003cp\u003e8.6 Novel Cross-linking Reagents for Mass Spectrometry.\u003c\/p\u003e \u003cp\u003e8.7 Analytical Software.\u003c\/p\u003e \u003cp\u003e8.8 Using Cross-linking Distance Constraints to Build Experimental Models of Protein Complexes.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9. Complex Formation in the Actin Cytoskeleton: Cross-linking Tools to Define Actin Protein Structure and Interactions (Sabrina Benchaar and Emil Reisler).\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e9.1 Introduction.\u003c\/p\u003e \u003cp\u003e9.2 Mapping Cross-linking with Methods Other than Mass Spectrometry.\u003c\/p\u003e \u003cp\u003e9.3 Actin-Actin Cross-linking.\u003c\/p\u003e \u003cp\u003e9.4 Intrastrand Cross-linked Actin between GLN41 and CYS374.\u003c\/p\u003e \u003cp\u003e9.5 Regulation of Cytoskeleton by ABPs and Mapping their Interfaces with Actin by Cross-linking.\u003c\/p\u003e \u003cp\u003e9.6 Cross-linking of Actin and Muscle Proteins—Examples of Experimental Approaches.\u003c\/p\u003e \u003cp\u003e9.7 Conclusing Remarks.\u003c\/p\u003e \u003cp\u003eAcknowledgement.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10. Computational Approaches to Examining Protein-Protein Interactions: Combining Experimental and Computational Data in the Era of Structural Genomics (J.K. Amisha Kamal).\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e10.1 Interactome in Structural Genomics.\u003c\/p\u003e \u003cp\u003e10.2 Importance of Computational Methods in Structural Genomics.\u003c\/p\u003e \u003cp\u003e10.3 Combining Computational Method with Experimental Data in Modeling the Structure of Protein Binary Complex.\u003c\/p\u003e \u003cp\u003e10.4 Method Summary.\u003c\/p\u003e \u003cp\u003e10.5 Experimental Methods.\u003c\/p\u003e \u003cp\u003eAcknowledgment.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11. Studies of Intact Proteins and Protein Complexes: ESI MS Approaches (Igor A. Kaltashov, Rinat R. Abzalimov, Agya K. Frimpong, and Stephen J. Eyles).\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e11.1 Introduction.\u003c\/p\u003e \u003cp\u003e11.2 Tertiary Structure Integrity and Conformational Heterogeneity (Charge State Distributions).\u003c\/p\u003e \u003cp\u003e11.3 Quaternary Structure Integrity and Composition of Non-Covalent Complexes.\u003c\/p\u003e \u003cp\u003e11.4 Functional Competence.\u003c\/p\u003e \u003cp\u003e11.5 Flexibility Maps and Binding Interfaces.\u003c\/p\u003e \u003cp\u003e11.6 Gas Phase Ion Chemistry and Its Influence of the Measurement of Protein Properties in Solution.\u003c\/p\u003e \u003cp\u003e11.7 challenges and Future Outlook.\u003c\/p\u003e \u003cp\u003eAcknowledgments.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12. Two Approaches to Mass Spectrometric Protein Footprinting: PLIMSTEX and FPOP (Michael L. Gross, Mei M. Zhu, and David M. Hambly).\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e12.1 Introduction: Protein-Ligand Interactions by Mass Spectrometry, Titration, and Hydrogen\/Deuterium Amide Exchange and Fast Photochemical Oxidation of Proteins.\u003c\/p\u003e \u003cp\u003e12.2 Protein-Ligand Interactions by Mass Spectrometry, Titration, and H\/D Amide Exchange (PLIMSTEX).\u003c\/p\u003e \u003cp\u003e12.3 Applications of PLMSTEX.\u003c\/p\u003e \u003cp\u003e12.4 Self-Association of Insulin: A Protein\/Protein Interaction.\u003c\/p\u003e \u003cp\u003e12.5 Features of PLMSTEX.\u003c\/p\u003e \u003cp\u003e12.6 Fast Photochemical Oxidation of Proteins: An Example of Fast Protein Footprinting.\u003c\/p\u003e \u003cp\u003e12.7 Features of FPOP.\u003c\/p\u003e \u003cp\u003e12.8 Future.\u003c\/p\u003e \u003cp\u003eAbbreviations.\u003c\/p\u003e \u003cp\u003eAcknowledgments.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003eIndex.\u003c\/p\u003e","brand":"John Wiley \u0026 Sons Inc","offers":[{"title":"Default Title","offer_id":49402309476695,"sku":"9780470258866","price":95.36,"currency_code":"GBP","in_stock":false}],"url":"https:\/\/bookcurl.com\/products\/mass-spectrometry-analysis-for-proteinprotein-interactions-and-dynamics-9780470258866","provider":"Book Curl","version":"1.0","type":"link"}