{"product_id":"laboratory-manual-for-biotechnology-and-laboratory-science-9781032419916","title":"Laboratory Manual for Biotechnology and","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003e\u003cp\u003eProvides the basic laboratory skills and knowledge to pursue a career in biotechnology. Written by four biotechnology instructors with over 20 years of teaching experience, it incorporates instruction, exercises, and laboratory activities that the authors have been using and perfecting for years. These exercises and activities help students understand the fundamentals of working in a biotechnology laboratory. Building skills through an organized and systematic presentation of materials, procedures, and tasks, the manual explores overarching themes that relate to all biotechnology workplaces including forensic, clinical, quality control, environmental, and other testing laboratories.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFeatures:\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eâ Provides clear instructions and step-by-step exercises to make learning the material easier for students.\u003c\/p\u003e\u003cp\u003e\u003ci\u003e There are Lab Notes for Instructors in the Support Material (see tab below).\u003c\/i\u003e\u003c\/p\u003e\u003cp\u003eâ Emphasizes fundamental laboratory skills that prepare students for th\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTrade Review\u003c\/b\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003eThis hefty, 1,171-page, paperback book is worth its weight in gold. What a fantastic reference for the various aspects of biotechnology. I loved the emphasis on the \"relationship between proper fundamental practices and reproducibility,\" and the authors' steadfast commitment to providing a \"solid grounding in basic, quality practices\" essential for success in biotechnology.\u003c\/p\u003e\u003cp\u003eThe sheer size and comprehensiveness of this book reinforces their introductory statement, \"..'biotechnology' is not a synonym for 'molecular biology.'\" Pretty much every possible test method used in a biotechnology setting (e.g., cell culture, chromatography, immunoassay) and their essential quality components (e.g., measurements such as weight and temperature; and solutions - love the declarative statement for section 24.1.1.2: \"water is not pure\") are described in great detail. Case studies accompany each chapter for the learners to test their retention and understanding. A nice inclusion is the section on regulatory affairs for learners to appreciate the challenges of bringing a biotechnology product to the consumer. \u003cbr\u003e\u003cbr\u003eThis is a fabulous textbook. If you work in biotechnology, this should be on every workbench and administrative office (e.g., supervisors, regulatory, quality assurance) for easy reference and, most importantly, adherence to\u003cbr\u003equality practices.\u003cbr\u003e\u003cbr\u003e\u003cem\u003eValerie L Ng, PhD MD(Alameda County Medical Center\/Highland Hospital)\u003c\/em\u003e\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cp\u003eUNIT I SAFETY IN THE LABORATORY\u003cbr\u003e\u003cbr\u003eUnit Introduction \u003c\/p\u003e\u003cp\u003eSAFETY PART 1: CREATING A SAFE WORKPLACE \u003c\/p\u003e\u003cp\u003eFundamental Principles \u003c\/p\u003e\u003cp\u003eClassroom Activity 1: Performing a Risk Assessment \u003c\/p\u003e\u003cp\u003eClassroom Activity 2: Exploring Safety-Related Government Web Sites \u003cbr\u003e\u003cbr\u003eClassroom Activity 3: Responding to Emergencies \u003c\/p\u003e\u003cp\u003eSAFETY PART 2: WORKING SAFELY WITH CHEMICALS\u003c\/p\u003e\u003cp\u003eFundamental Principles \u003c\/p\u003e\u003cp\u003eClassroom Activity 4: Understanding the Chemicals with Which You Work \u003cbr\u003e\u003cbr\u003eClassroom Activity 5: Personal Protection \u003c\/p\u003e\u003cp\u003eLaboratory Exercise 1: Tracking the Spread of Chemical Contamination \u003c\/p\u003e\u003cp\u003eClassroom Activity 6: Analyzing Safety Issues in a Laboratory Procedure \u003cbr\u003e \u003cbr\u003eSAFETY PART 3: WORKING SAFELY WITH BIOLOGICAL HAZARDS\u003c\/p\u003e\u003cp\u003eFundamental Principles \u003c\/p\u003e\u003cp\u003eLaboratory Exercise 2: Production of Bioaerosols and Factors Affecting Aerosol \u003cbr\u003e \u003cbr\u003eProduction \u003cbr\u003e\u003cbr\u003eUnit Discussion \u003cbr\u003e\u003cbr\u003eSafety Contract, student copy \u003cbr\u003e\u003cbr\u003eSafety Contract, to turn in \u003c\/p\u003e\u003cp\u003eUNIT II DOCUMENTATION IN THE LABORATORY \u003c\/p\u003e\u003cp\u003eUnit Introduction \u003c\/p\u003e\u003cp\u003eClassroom Activity 7: Being an Auditor \u003cbr\u003e\u003cbr\u003eLaboratory Exercise 3: Keeping a Laboratory Notebook \u003c\/p\u003e\u003cp\u003eClassroom Activity 8: Writing and Following an SOP \u003c\/p\u003e\u003cp\u003eUnit Discussion \u003cbr\u003e \u003cbr\u003eUNIT III METROLOGY IN THE LABORATORY \u003c\/p\u003e\u003cp\u003eUnit Introduction \u003c\/p\u003e\u003cp\u003eLaboratory Exercise 4: Recording Measurements with the Correct Number of Significant \u003cbr\u003e Figures \u003cbr\u003eClassroom Activity 9: Constructing a Simple Balance \u003c\/p\u003e\u003cp\u003eLaboratory Exercise 5: Weight Measurements 1: Good Weighing Practices \u003c\/p\u003e\u003cp\u003eLaboratory Exercise 6: Weight Measurements 2: Performance Verification \u003c\/p\u003e\u003cp\u003eLaboratory Exercise 7: Volume Measurements 1: Proper Use of Volume \u003cbr\u003e Measuring Devices \u003c\/p\u003e\u003cp\u003eLaboratory Exercise 8: Volume Measurements 2: Performance Verification of a M Micropipette \u003c\/p\u003e\u003cp\u003eLaboratory Exercise 9: Measuring pH with Accuracy and Precision \u003c\/p\u003e\u003cp\u003eUnit Discussion \u003c\/p\u003e\u003cp\u003eUNIT IV SPECTROPHOTOMETRY AND THE MEASUREMENT OF LIGHT \u003c\/p\u003e\u003cp\u003eUnit Introduction \u003c\/p\u003e\u003cp\u003eLaboratory Exercise 10: Color and the Absorbance of Light \u003cbr\u003e\u003cbr\u003eLaboratory Exercise 11: Concentration, Absorbance, and Transmittance \u003cbr\u003e\u003cbr\u003eLaboratory Exercise 12: Preparing a Standard Curve With Food Coloring and Using it for Quantitation \u003cbr\u003e\u003cbr\u003eClassroom Activity 10: Beer’s Law and Calculating an Absorptivity Constant \u003cbr\u003e\u003cbr\u003eLaboratory Exercise 13: Determination of the Absorptivity Constant for ONP \u003cbr\u003e\u003cbr\u003eUnit Discussion \u003c\/p\u003e\u003cp\u003eUNIT V BIOLOGICAL SOLUTIONS \u003c\/p\u003e\u003cp\u003eUnit Introduction \u003cbr\u003e \u003cbr\u003eClassroom Activity 11: Getting Ready to Prepare Solutions with One Solute: Calculations \u003c\/p\u003e\u003cp\u003eClassroom Activity 12: Getting Ready to Prepare Solutions with One Solute: Ordering \u003cbr\u003e Chemicals \u003cbr\u003e\u003cbr\u003eLaboratory Exercise 14: Preparing Solutions with One Solute \u003cbr\u003e\u003cbr\u003eLaboratory Exercise 15: Preparing Solutions to the Correct Concentration \u003cbr\u003e\u003cbr\u003eLaboratory Exercise 16: Working with Buffers \u003cbr\u003e \u003cbr\u003eLaboratory Exercise 17: Preparing Breaking Buffer \u003cbr\u003e\u003cbr\u003eLaboratory Exercise 18: Preparing TE Buffer \u003cbr\u003e\u003cbr\u003eLaboratory Exercise 19: More Practice Making a Buffer \u003cbr\u003e\u003cbr\u003eLaboratory Exercise 20: Making a Quality Product in a Simulated Company \u003cbr\u003e\u003cbr\u003eUnit Discussion \u003c\/p\u003e\u003cp\u003eUNIT VI ASSAYS \u003cbr\u003e \u003cbr\u003eUnit Introduction \u003c\/p\u003e\u003cp\u003eLaboratory Exercise 21: Two Qualitative Assays \u003cbr\u003e\u003cbr\u003eLaboratory Exercise 22: UV Spectrophotometric Assay of DNA: Quantitative Application \u003c\/p\u003e\u003cp\u003eLaboratory Exercise 23: UV Spectrophotometric Assay of DNA and Proteins: Qualitative Applications\u003cbr\u003e\u003cbr\u003eLaboratory Exercise 24: The Bradford Protein Assay: Learning the Assay \u003cbr\u003eLaboratory Exercise 25: The Bradford Protein Assay: Exploring Assay Verification \u003cbr\u003eLaboratory Exercise 26: The Beta-Galactosidase Enzyme Assay \u003cbr\u003e Laboratory Exercise 27: Comparing the Specific Activity of Two Preparations of \u003cbr\u003e Beta-Galactosidase\u003cbr\u003eLaboratory Exercise 28: Using Spectrophotometry for Quality Control: Niacin \u003cbr\u003eUnit Discussion \u003cbr\u003eUNIT VII BIOLOGICAL SEPARATION METHODS \u003c\/p\u003e\u003cp\u003eUnit Introduction \u003c\/p\u003e\u003cp\u003eClassroom Activity 13: Planning for Separating Materials Using a Centrifuge \u003cbr\u003eLaboratory Exercise 29: Separation of Two Substances Based on Their Differential \u003cbr\u003e Affinities for Two Phases \u003cbr\u003eLaboratory Exercise 30: Separation and Identification of Dyes Using Paper \u003cbr\u003e Chromatography \u003cbr\u003eLaboratory Exercise 31: Separating Molecules by Agarose Gel Electrophoresis \u003cbr\u003eLaboratory Exercise 32: Using Agarose Gel Electrophoresis to Perform an Assay \u003cbr\u003eLaboratory Exercise 33: Optimizing Agarose Gel Electrophoresis \u003c\/p\u003e\u003cp\u003eLaboratory Exercise 34: Quantification of DNA by Agarose Gel Electrophoresis \u003cbr\u003eLaboratory Exercise 35: Introduction to Ion Exchange Column Chromatography \u003c\/p\u003e\u003cp\u003eUnit Discussion \u003c\/p\u003e\u003cp\u003eUNIT VIII GROWING CELLS \u003c\/p\u003e\u003cp\u003eUnit Introduction \u003c\/p\u003e\u003cp\u003eGROWING CELLS PART I: BACTERIAL CELLS\u003c\/p\u003e\u003cp\u003eLaboratory Exercise 36: Using a Compound Light Microscope \u003cbr\u003e\u003cbr\u003eLaboratory Exercise 37: Aseptic Technique on an Open Lab Bench \u003cbr\u003e\u003cbr\u003eLaboratory Exercise 38: Working with Bacteria on an Agar Substrate: Isolating \u003cbr\u003e Individual Colonies \u003cbr\u003e\u003cbr\u003eLaboratory Exercise 39: Gram Staining \u003c\/p\u003e\u003cp\u003eLaboratory Exercise 40: Preparing Phosphate-Buffered Saline \u003cbr\u003e\u003cbr\u003eLaboratory Exercise 41: The Aerobic Spread-Plate Method of Enumerating Colony- \u003cbr\u003e \u003cbr\u003e Forming Units\u003cbr\u003e\u003cbr\u003eLaboratory Exercise 42: Preparing a Growth Curve for E. coli \u003c\/p\u003e\u003cp\u003eGROWING CELLS PART 2: MAMMALIAN CELLS\u003c\/p\u003e\u003cp\u003eLaboratory Exercise 43: Aseptic Technique in a Biological Safety Cabinet \u003cbr\u003e\u003cbr\u003eLaboratory Exercise 44: Making Ham’s F12 Medium from Dehydrated Powder \u003cbr\u003e\u003cbr\u003eLaboratory Exercise 45: Examining, Photographing, and Feeding CHO Cells \u003cbr\u003e\u003cbr\u003eLaboratory Exercise 46: Counting Cells Using a Hemocytometer \u003cbr\u003e\u003cbr\u003eLaboratory Exercise 47: Subculturing CHO Cells \u003c\/p\u003e\u003cp\u003eLaboratory Exercise 48: Preparing a Growth Curve for CHO Cells \u003cbr\u003e\u003cbr\u003eUnit Discussion \u003c\/p\u003e\u003cp\u003eAPPENDICES \u003cbr\u003e\u003cbr\u003e1. Abbreviations and Acronyms Used in this Laboratory Manual \u003cbr\u003e \u003cbr\u003e2. Glossary \u003c\/p\u003e\u003cp\u003e3. Selected Bibliography \u003c\/p\u003e\u003cp\u003e4. Brief Metric Review \u003c\/p\u003e\u003cp\u003e5. Calculating Standard Deviation \u003c\/p\u003e\u003cp\u003e6. Equipment, Supplies, and Reagents Required for Each Unit \u003c\/p\u003e\u003cp\u003e7. Recipes and Preparation Notes \u003cbr\u003e \u003c\/p\u003e","brand":"CRC Press","offers":[{"title":"Default Title","offer_id":51019042455895,"sku":"9781032419916","price":999.99,"currency_code":"GBP","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9781032419916.jpg?v=1750779126","url":"https:\/\/bookcurl.com\/products\/laboratory-manual-for-biotechnology-and-laboratory-science-9781032419916","provider":"Book Curl","version":"1.0","type":"link"}