{"product_id":"laboratory-exercises-and-techniques-in-cellular-biology-9780470631232","title":"Laboratory Exercises and Techniques in Cellular","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003eThis manual consists of 30+ experiments that coincide and complement each of the 18 chapters in the Karp text (978-1-1182-0673-7.) There are three possible designs of the lab book, based on the instructor''s needs. These designs focus on either Techniques, Concepts, or Organelles. The procedures of the 30+ experiments remain standard and unchanged in all designs of the lab book. Special Overview pages, Discussion Questions and Datasheets bookend the procedures in order to create each of the possible textbook designs. This gives instructors flexibility to create a lab book that suits their lecture course curriculum, their experience, and available equipment and supplies.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cp\u003eIntroduction vii\u003c\/p\u003e \u003cp\u003eAcknowledgment ix\u003c\/p\u003e \u003cp\u003e1 Introduction and Microscopy 1\u003cbr\u003e(Karp chapter 1 \u0026amp; 18)\u003c\/p\u003e \u003cp\u003e2 Enzyme Kinetics 11\u003cbr\u003e(Karp chapter 3 \u0026amp; 11)\u003c\/p\u003e \u003cp\u003e3 Membrane Permeability 19\u003cbr\u003e(Karp chapter 4)\u003c\/p\u003e \u003cp\u003e4 Protein Transport to the Plasma Membrane 27\u003cbr\u003e(Karp chapter 4 \u0026amp; 8)\u003c\/p\u003e \u003cp\u003e5 Cell Fractionation 33\u003cbr\u003e(Karp chapter 5 \u0026amp; 12)\u003c\/p\u003e \u003cp\u003e6 Isolation and Activity of Mitochondria 41\u003cbr\u003e(Karp chapter 3 \u0026amp; 5)\u003c\/p\u003e \u003cp\u003e7 Staining of the Extracellular Space 49\u003cbr\u003e(Karp chapter 7)\u003c\/p\u003e \u003cp\u003e8 Protoplast Isolation and Vacuole Staining 57\u003cbr\u003e(Karp chapter 8)\u003c\/p\u003e \u003cp\u003e9 Isolation of Vacuoles 65\u003cbr\u003e(Karp chapter 8)\u003c\/p\u003e \u003cp\u003e10 Staining of Autophagosomes and Lysosomes 71\u003cbr\u003e(Karp chapter 8)\u003c\/p\u003e \u003cp\u003e11 Staining of the Cytoskeleton 77\u003cbr\u003e(Karp chapter 9)\u003c\/p\u003e \u003cp\u003e12 Cell Motility 85\u003cbr\u003e(Karp chapter 9)\u003c\/p\u003e \u003cp\u003e13 Staining of the Nucleus and Nucleic Acids 95\u003cbr\u003e(Karp chapter 12)\u003c\/p\u003e \u003cp\u003e14 Staining of Chromosome and Cell Cycle Analysis 105\u003cbr\u003e(Karp chapter 14)\u003c\/p\u003e \u003cp\u003e15 Staining for Cell Viability and Apoptosis 113\u003cbr\u003e(Karp chapter 15 \u0026amp; 16)\u003c\/p\u003e \u003cp\u003e16 Staining of Immune Cells 121\u003cbr\u003e(Karp chapter 17)\u003c\/p\u003e \u003cp\u003e17 Protein Isolation, Fractionation, and SDS-PAGE 131\u003cbr\u003e(Karp chapter 18)\u003c\/p\u003e \u003cp\u003e18 Genomic DNA Isolation, Purification, and the Polymerase Chain Reaction 139\u003cbr\u003e(Karp chapter 18)\u003c\/p\u003e \u003cp\u003e19 Ligation and Cloning 145\u003cbr\u003e(Karp chapter 18)\u003c\/p\u003e \u003cp\u003e20 Restriction Fragment Length Polymorphism Analysis and Agarose Gel Electrophoresis 151\u003cbr\u003e(Karp chapter 10 \u0026amp; 18)\u003c\/p\u003e \u003cp\u003e21 Staining of Peroxisomes* 157\u003cbr\u003e(Karp chapter 5)\u003c\/p\u003e \u003cp\u003e22 Short-Term Aggregation Culture* 163\u003cbr\u003e(Karp chapter 7)\u003c\/p\u003e \u003cp\u003e23 Staining of the Endoplasmic Reticulum and the Golgi Apparatus* 171\u003cbr\u003e(Karp chapter 8)\u003c\/p\u003e \u003cp\u003e24 The Endocytic Pathway (FM4-64 Staining)* 179\u003cbr\u003e(Karp chapter 4 \u0026amp; 8)\u003c\/p\u003e \u003cp\u003e25 Detection of MAP Kinase Signaling* 187\u003cbr\u003e(Karp chapter 15)\u003c\/p\u003e \u003cp\u003e26 Cancer* 193\u003cbr\u003e(Karp chapter 16)\u003c\/p\u003e \u003cp\u003e27 Western Blot* 197\u003cbr\u003e(Karp chapter 18)\u003c\/p\u003e \u003cp\u003e28 Enzyme-Linked Immunosorbent Assay* 203\u003cbr\u003e(Karp chapter 18)\u003c\/p\u003e \u003cp\u003e29 Southern and Northern Blotting* 211\u003cbr\u003e(Karp chapter 18)\u003c\/p\u003e \u003cp\u003e30 Using Online Research Tools* 217\u003cbr\u003e(Karp chapter 10 \u0026amp; 11)\u003c\/p\u003e \u003cp\u003eAppendix A: A Guide to Staining Techniques \u0026amp; Photomicroscopy \u003cbr\u003e(Available online at www.wiley.com\/college\/contento)\u003c\/p\u003e \u003cp\u003eAppendix B: Careers in Cell Biology\u003cbr\u003e(Available online at www.wiley.com\/college\/contento)\u003c\/p\u003e \u003cp\u003eInstructor’s Notes\u003cbr\u003e(Available online at www.wiley.com\/college\/contento)\u003c\/p\u003e \u003cp\u003eIndex 223\u003c\/p\u003e","brand":"John Wiley \u0026 Sons Inc","offers":[{"title":"Default Title","offer_id":49525383070039,"sku":"9780470631232","price":107.3,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9780470631232.jpg?v=1731860305","url":"https:\/\/bookcurl.com\/products\/laboratory-exercises-and-techniques-in-cellular-biology-9780470631232","provider":"Book Curl","version":"1.0","type":"link"}