{"product_id":"lab-exercises-in-microbiology-5e-student-choice-9781119462668","title":"Lab Exercises in Microbiology 5e Student Choice","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cp\u003ePreface iii\u003c\/p\u003e \u003cp\u003eIntroduction: Laboratory Operations and Safety xiii\u003c\/p\u003e \u003cp\u003eBasic Rules for Microbiology Laboratory Safety xv\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart 1 General Microscopy and Aseptic Technique\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Laboratory Safety: Introduction to the Microscope 3\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eLaboratory Safety and Procedures 4\u003c\/p\u003e \u003cp\u003eLaboratory Procedures 4\u003c\/p\u003e \u003cp\u003eSimple Stain Technique 5\u003c\/p\u003e \u003cp\u003eThe Microscope 6\u003c\/p\u003e \u003cp\u003eMicroscope Components (Plate 1) 7\u003c\/p\u003e \u003cp\u003eEukaryotic Versus Prokaryotic Cells (Plates 2A, 2B) 10\u003c\/p\u003e \u003cp\u003eLaboratory Cleanup 10\u003c\/p\u003e \u003cp\u003eMicroscope Cleanup 10\u003c\/p\u003e \u003cp\u003eDiscards 11\u003c\/p\u003e \u003cp\u003eGeneral Cleanup 11\u003c\/p\u003e \u003cp\u003eSpeaking of Safety 11\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Transfer and Isolation Techniques, Microbes in the Environment 17\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eTransfer Technique 17\u003c\/p\u003e \u003cp\u003eTube-to-Tube Transfers 18\u003c\/p\u003e \u003cp\u003eBasic Aseptic Technique Procedures 18\u003c\/p\u003e \u003cp\u003eIsolation Techniques: Streak Plate and Pour Plate (Optional) 22\u003c\/p\u003e \u003cp\u003eStreak Plate (Plate 3) 22\u003c\/p\u003e \u003cp\u003ePrepare the Following Streak Plates Using the Cultures Provided 25\u003c\/p\u003e \u003cp\u003eTo Lap or Not to Lap— That is the Question 25\u003c\/p\u003e \u003cp\u003ePour Plate (Plate 4) Optional, May be Done as a Demonstration 25\u003c\/p\u003e \u003cp\u003eMicrobes in the Environment 26\u003c\/p\u003e \u003cp\u003eHuman Environment: Procedure 27\u003c\/p\u003e \u003cp\u003eClassroom\/School Environment: Procedure 27\u003c\/p\u003e \u003cp\u003eIncubation 27\u003c\/p\u003e \u003cp\u003eResults 27\u003c\/p\u003e \u003cp\u003eLaboratory Cleanup 28\u003c\/p\u003e \u003cp\u003eDiscards 28\u003c\/p\u003e \u003cp\u003eGeneral Cleanup 28\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart 2 Microbial Morphology, Differential Stains\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Cultural and Cellular Morphology 33\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eCultural Characteristics of Bacteria 33\u003c\/p\u003e \u003cp\u003eProcedure: Plates (Plate 5) 34\u003c\/p\u003e \u003cp\u003eProcedure: Agar Slants 35\u003c\/p\u003e \u003cp\u003eProcedure: Broth (Plate 6) 35\u003c\/p\u003e \u003cp\u003eMicrobial Cellular Morphology 35\u003c\/p\u003e \u003cp\u003eSmear Preparation 36\u003c\/p\u003e \u003cp\u003eBroth Preparation 37\u003c\/p\u003e \u003cp\u003eAgar Slant and Plate Preparation 37\u003c\/p\u003e \u003cp\u003eSimple Stain Procedure 37\u003c\/p\u003e \u003cp\u003eResults from the Simple Stain Procedure (Plates 7–16) 38\u003c\/p\u003e \u003cp\u003eResults from Prepared Slide 38\u003c\/p\u003e \u003cp\u003eLaboratory Cleanup 38\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Bacterial Growth 43\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eFactors Needed for Bacterial Growth 44\u003c\/p\u003e \u003cp\u003eMeasuring Bacterial Growth 45\u003c\/p\u003e \u003cp\u003eProcedure for Preparation of Spread Plate 45\u003c\/p\u003e \u003cp\u003eProcedure for Demonstration of a Bacterial Growth Curve 47\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Gram Stain and Acid-Fast Stain 53\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eThe Gram Stain 53\u003c\/p\u003e \u003cp\u003eThe Gram Stain Technique (Traditional Method) (Plate 17A, B, C) 54\u003c\/p\u003e \u003cp\u003eGram Stain: (Alternative Method) 55\u003c\/p\u003e \u003cp\u003eThe Acid-Fast Stain Technique (Plate 18) 56\u003c\/p\u003e \u003cp\u003eAcid-Fast Technique 56\u003c\/p\u003e \u003cp\u003eLaboratory Cleanup 58\u003c\/p\u003e \u003cp\u003eIncubation 58\u003c\/p\u003e \u003cp\u003eDiscards 58\u003c\/p\u003e \u003cp\u003eGeneral Cleanup 58\u003c\/p\u003e \u003cp\u003eSlides and Microscopes 58\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Endospore Stain, Capsule Stain, and the Hanging Drop Technique 61\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eThe Endospore Stain 61\u003c\/p\u003e \u003cp\u003eThe Spore Stain Technique (Plates 19, 20) 62\u003c\/p\u003e \u003cp\u003eResults 63\u003c\/p\u003e \u003cp\u003eThe Capsule Stain 63\u003c\/p\u003e \u003cp\u003eThe Capsule Stain Technique (Plate 21) 63\u003c\/p\u003e \u003cp\u003eThe Hanging Drop Technique 64\u003c\/p\u003e \u003cp\u003eResults 64\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Fungi 69\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eThe Fungi 69\u003c\/p\u003e \u003cp\u003eResults 72\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Viruses—Visualization and Enumeration 77\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eViruses 77\u003c\/p\u003e \u003cp\u003eBacteriophage Enumeration 78\u003c\/p\u003e \u003cp\u003eResults 80\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Parasitology 85\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart 3 Microbial Control and Biochemistry\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Microbial Sensitivity Testing 97\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003ePart 1: Physical Methods Ultraviolet Light Sensitivity 98\u003c\/p\u003e \u003cp\u003eSterilization by the Use of UV Light 98\u003c\/p\u003e \u003cp\u003eThe Penetrating Power of UV Light (Plate 34) 99\u003c\/p\u003e \u003cp\u003eHeat Sensitivity (may be done as an alternative to the UV light procedure) 101\u003c\/p\u003e \u003cp\u003eEffect of Cold Temperature and Slow Freezing (Optional) 102\u003c\/p\u003e \u003cp\u003ePart 2: Chemical Methods Chemical Sensitivity 102\u003c\/p\u003e \u003cp\u003eChemotherapeutic Agent Testing: The Kirby–Bauer Plate 103\u003c\/p\u003e \u003cp\u003eThe Kirby–Bauer Technique 104\u003c\/p\u003e \u003cp\u003eResults 107\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Bacterial Biochemistry 113\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eCarbohydrate Metabolizing Enzymes 114\u003c\/p\u003e \u003cp\u003eResults 115\u003c\/p\u003e \u003cp\u003eCarbohydrate Fermentation (Phenol Red Broth) Tubes (Plate 37) 115\u003c\/p\u003e \u003cp\u003eAmino Acid and Nitrogen Metabolism 116\u003c\/p\u003e \u003cp\u003eResults 117\u003c\/p\u003e \u003cp\u003eDecarboxylase Tubes (Plate 42) 117\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Gas Requirements of Microorganisms 123\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eGrowth of Anaerobes (may be done as a demonstration) 124\u003c\/p\u003e \u003cp\u003eDemonstration of Catalase 125\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Specialized Media 129\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eExamples 130\u003c\/p\u003e \u003cp\u003eInoculation of Blood Agar Plates for Demonstration of Alpha Hemolysis and Transport Medium 131\u003c\/p\u003e \u003cp\u003eInoculation of Blood, Phenylethyl Alcohol, Mannitol Salt, Macconkey, and Eosin–Methylene Blue Agar Plates 132\u003c\/p\u003e \u003cp\u003eResults 133\u003c\/p\u003e \u003cp\u003eInoculation of Triple Sugar Iron Agar and Sulfide–Indole Motility Medium 133\u003c\/p\u003e \u003cp\u003eResults 134\u003c\/p\u003e \u003cp\u003eResults of Transport Medium 134\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart 4 Medical Microbiology\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14 Genetics 143\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eAmes Test 144\u003c\/p\u003e \u003cp\u003eUV Light Procedure 145\u003c\/p\u003e \u003cp\u003eKirby–Bauer Procedure 145\u003c\/p\u003e \u003cp\u003eResults: Ames Test (Plate 55) 145\u003c\/p\u003e \u003cp\u003eResults: UV Light Procedure (Plate 35) 146\u003c\/p\u003e \u003cp\u003eResults: Kirby–Bauer Test (Plates 36, 55) 146\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15 Epidemiology 149\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eHandwashing Procedure (Done with Three Students) 150\u003c\/p\u003e \u003cp\u003eResults: Handwashing (Plate 56) 151\u003c\/p\u003e \u003cp\u003eFomite and Direct Transmission of Microbes 151\u003c\/p\u003e \u003cp\u003eResults: Fomite and Direct Transmission 152\u003c\/p\u003e \u003cp\u003eAirborne Infections: Cough and Sneeze Plates 152\u003c\/p\u003e \u003cp\u003eResults: Airborne and Cough\/Sneeze Plates 153\u003c\/p\u003e \u003cp\u003eMicrobes in Makeup (Optional) 153\u003c\/p\u003e \u003cp\u003eResults: Microbes in Makeup 153\u003c\/p\u003e \u003cp\u003e\u003cb\u003e16 Specimen-Handling Protocols 159\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eQuantitative Urinalysis 160\u003c\/p\u003e \u003cp\u003eResults: Urine Samples 162\u003c\/p\u003e \u003cp\u003eSpecimens from the Gastrointestinal Tract 163\u003c\/p\u003e \u003cp\u003eResults: GI Tract Isolation Technique 163\u003c\/p\u003e \u003cp\u003e\u003cb\u003e17 Specific Laboratory Tests 169\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eCatalase Test 171\u003c\/p\u003e \u003cp\u003eAlternative Method (to be done at the end of this exercise) 171\u003c\/p\u003e \u003cp\u003eResults: Catalase Test (Plate 46) 171\u003c\/p\u003e \u003cp\u003eBacitracin Sensitivity—Demonstration 171\u003c\/p\u003e \u003cp\u003eResults: Bacitracin Sensitivity (Plate 57) 172\u003c\/p\u003e \u003cp\u003eOxidase Test 172\u003c\/p\u003e \u003cp\u003eResults: Oxidase Test (Plate 59) 172\u003c\/p\u003e \u003cp\u003eCoagulase Test 172\u003c\/p\u003e \u003cp\u003eRapid Slide Test 172\u003c\/p\u003e \u003cp\u003eTube Test 172\u003c\/p\u003e \u003cp\u003eResults: Coagulase Tube Test (Plate 60) 172\u003c\/p\u003e \u003cp\u003eNovobiocin Sensitivity 172\u003c\/p\u003e \u003cp\u003eResults: Novobiocin Sensitivity 173\u003c\/p\u003e \u003cp\u003eCamp Test (Plate 58) 173\u003c\/p\u003e \u003cp\u003eResults: Camp 173\u003c\/p\u003e \u003cp\u003e\u003cb\u003e18 Serology 179\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eElisa Test: Test for Toxins A and B from Clostridium Difficile 180\u003c\/p\u003e \u003cp\u003eResults 182\u003c\/p\u003e \u003cp\u003eRapid Identification of Group A Antigen 182\u003c\/p\u003e \u003cp\u003eResults 184\u003c\/p\u003e \u003cp\u003eDifferentiation of Streptococci Using Latex Agglutination 184\u003c\/p\u003e \u003cp\u003eSlidex Strepto-Kit® 184\u003c\/p\u003e \u003cp\u003eResults 186\u003c\/p\u003e \u003cp\u003eProcedure Controls 186\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart 5 Identification of a Bacterial Unknown\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e19 Identification of Enteric Pathogens: Traditional Methods 191\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eResults 194\u003c\/p\u003e \u003cp\u003eIMV\u003csub\u003eI\u003c\/sub\u003eC Test (See Plate 61) 194\u003c\/p\u003e \u003cp\u003eResults of Gelatin Hydrolysis 194\u003c\/p\u003e \u003cp\u003eResults of the Oxidase Test (See Plate 59) 194\u003c\/p\u003e \u003cp\u003e\u003cb\u003e20 Identification of Enteric Pathogens: Rapid Identification Methods 201\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eEnteroPluri Test (Plate 62) 201\u003c\/p\u003e \u003cp\u003eCompartment 1: Glucose Fermentation and Gas Production 202\u003c\/p\u003e \u003cp\u003eCompartments 2 and 3: Lysine and Ornithine Decarboxylation 202\u003c\/p\u003e \u003cp\u003eCompartment 4: Sulfide and Indole Production 202\u003c\/p\u003e \u003cp\u003eCompartments 5–8: Adonitol, Lactose, Arabinose, and Sorbitol Fermentation 202\u003c\/p\u003e \u003cp\u003eCompartment 9: Voges–Proskauer 202\u003c\/p\u003e \u003cp\u003eCompartment 10: Phenylalanine Deaminase and Dulcitol 202\u003c\/p\u003e \u003cp\u003eCompartment 11: Urea 202\u003c\/p\u003e \u003cp\u003eCompartment 12: Citrate 202\u003c\/p\u003e \u003cp\u003eApi® 20 E System (Plate 63) 203\u003c\/p\u003e \u003cp\u003eMicrotube 1: Ortho-Nitrophenyl-Beta-D-Ortho-Nitrophenyl-Beta-D-Galactopyranoside (Onpg) 203\u003c\/p\u003e \u003cp\u003eMicrotubes 2–4: Arginine Dihydrolase (Decarboxylase), Lysine Decarboxylase, and Ornithine Decarboxylase (Adh, Ldc, Odc) 203\u003c\/p\u003e \u003cp\u003eMicrotube 5: Citrate (Cit) 203\u003c\/p\u003e \u003cp\u003eMicrotube 6: Sulfide (H\u003csub\u003e2\u003c\/sub\u003es) 203\u003c\/p\u003e \u003cp\u003eMicrotube 7: Urea (Ure) 203\u003c\/p\u003e \u003cp\u003eMicrotube 8: Tryptophan Deaminase (Tda) 203\u003c\/p\u003e \u003cp\u003eMicrotube 9: Indole (Ind) 203\u003c\/p\u003e \u003cp\u003eMicrotube 10: Voges–Proskauer (Vp) 203\u003c\/p\u003e \u003cp\u003eMicrotube 11: Gelatin Hydrolysis (Gel) 203\u003c\/p\u003e \u003cp\u003eMicrotubes 12–20: Utilization of the Carbohydrates Glucose, Mannitol, Inositol, Sorbitol, Rhamnose, Saccharose (Sucrose), Melibiose, Amygdalin, and Arabinose (Glu, Man, Ino, Sor, Rha, Sac, Mel, Amy, And Ara) 203\u003c\/p\u003e \u003cp\u003eInoculation of Rapid Identification Media 204\u003c\/p\u003e \u003cp\u003eInoculation of EnteroPluri Test 204\u003c\/p\u003e \u003cp\u003eInoculation of the Api 20 E System 204\u003c\/p\u003e \u003cp\u003eResults 205\u003c\/p\u003e \u003cp\u003e\u003cb\u003e21 Identification of a Bacterial Unknown: The Gram-Negative Unknown 209\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eReview 209\u003c\/p\u003e \u003cp\u003eComparison Chart 210\u003c\/p\u003e \u003cp\u003eFlowchart 210\u003c\/p\u003e \u003cp\u003eComputer Identification 210\u003c\/p\u003e \u003cp\u003eID Value Worksheet for EnteroPluri Test II 212\u003c\/p\u003e \u003cp\u003eResults 213\u003c\/p\u003e \u003cp\u003e\u003cb\u003e22 Identification of a Bacterial Unknown: The Gram-Positive Cocci 217\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart 6 Food and Environmental Microbiology\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e23 Identification and Quantitation of Microbial Numbers in a Water Sample 225\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003ePresumptive Test: Analysis of a Contaminated Water Sample for the Presence of Coliforms 226\u003c\/p\u003e \u003cp\u003eResults 227\u003c\/p\u003e \u003cp\u003eQuantitation of Microbial Number in a Water Sample 227\u003c\/p\u003e \u003cp\u003eResults (Plate 64) 228\u003c\/p\u003e \u003cp\u003e\u003cb\u003e24 Identification of Microbes in Beef and Poultry and the Quantitation of Microbial Numbers 231\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eMicrobial Presence in a Food Product and Colony Counts 232\u003c\/p\u003e \u003cp\u003eResults 233\u003c\/p\u003e \u003cp\u003eRecognition of Organism Genera 233\u003c\/p\u003e \u003cp\u003e\u003cb\u003e25 Soil Microbiology 237\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003ePart 1: The Recovery of Microorganisms from Soil 238\u003c\/p\u003e \u003cp\u003eResults 239\u003c\/p\u003e \u003cp\u003eIsolation Of Soil Bacteria 239\u003c\/p\u003e \u003cp\u003eResults 239\u003c\/p\u003e \u003cp\u003ePart 2: Isolation of Soil Bacteriophages of Bacillus Subtilis 239\u003c\/p\u003e \u003cp\u003eResults 240\u003c\/p\u003e \u003cp\u003e\u003cb\u003e26 Microbial Ecology 245\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eCompetition Between Bacteria 246\u003c\/p\u003e \u003cp\u003eResults 246\u003c\/p\u003e \u003cp\u003eBacteriocin Production 246\u003c\/p\u003e \u003cp\u003eResults 247\u003c\/p\u003e \u003cp\u003eBacterial–Fungal Interaction (Plate 65) 247\u003c\/p\u003e \u003cp\u003eResults 247\u003c\/p\u003e \u003cp\u003e\u003cb\u003e27 Biofilms 251\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eGrowth of Dental Plaque in situ 252\u003c\/p\u003e \u003cp\u003eResults 253\u003c\/p\u003e \u003cp\u003eSoil Biofilm 253\u003c\/p\u003e \u003cp\u003eResults 253\u003c\/p\u003e \u003cp\u003eGrowth of Bacterial Biofilms in vitro 253\u003c\/p\u003e \u003cp\u003eResults 254\u003c\/p\u003e \u003cp\u003eBibliography 261\u003c\/p\u003e \u003cp\u003eAnswer Key for the Exercise Pre-Tests 263\u003c\/p\u003e \u003cp\u003eAppendix 1 Flow Charts and Comparison Charts for the Identification of Enteric Gram Negative Rods Using Traditional Testing Procedures 265\u003c\/p\u003e \u003cp\u003eAppendix 2 Flow Chart for the Identification of Enterobacteriaceae based on the API 20 E Rapid Identification System 269\u003c\/p\u003e \u003cp\u003eAppendix 3 Gram-Positive Flow Chart 275\u003c\/p\u003e \u003cp\u003ePhoto Credit List 277\u003c\/p\u003e \u003cp\u003eIndex 279\u003c\/p\u003e \u003cp\u003ePhotographic Atlas PA-1\u003c\/p\u003e","brand":"John Wiley \u0026 Sons Inc","offers":[{"title":"Default Title","offer_id":49528856412503,"sku":"9781119462668","price":110.66,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9781119462668.jpg?v=1731873295","url":"https:\/\/bookcurl.com\/products\/lab-exercises-in-microbiology-5e-student-choice-9781119462668","provider":"Book Curl","version":"1.0","type":"link"}