{"product_id":"integrated-genomics-9780470095027","title":"Integrated Genomics","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003eIntegrated Genomics: A Discovery-Based Laboratory Course introduces the excitement of discovery to the basic molecular biology laboratory. Utilizing up-to-date molecular biology protocols and a basic experimental design, this text offers experience with three different model systems.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTrade Review\u003c\/b\u003e\u003cbr\u003e\"I greatly admire the efforts of the authors. Their goals are praiseworthy and this manual is well written.\" (\u003ci\u003eThe Quarterly Review of Biology\u003c\/i\u003e, September 2007)  \u003cp\u003e\"…an excellent tool for introducing a wide range of students to modern concepts in molecular biology, genomics, proteomics, and bioinformatics in an integrated discovery-based format…\" (\u003ci\u003eThe Annals of Pharmacotherapy\u003c\/i\u003e, March 2007)\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cb\u003ePreface.\u003c\/b\u003e  \u003cp\u003e\u003cb\u003eAuthor biographies.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAcknowledgments.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eList of figures.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Introduction to basic laboratory genetics.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1.1 Transferring and handling \u003ci\u003eC. elegans.\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e1.2 Introduction to laboratory genetics.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Gene expression analysis using transgenic animals.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 Transgenic gene expression analysis in \u003ci\u003eC. elegans: lacZ\u003c\/i\u003e staining.\u003c\/p\u003e \u003cp\u003e2.2 Transgenic gene expression analysis in \u003ci\u003eC. elegans:\u003c\/i\u003e GFP analysis.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Creation and testing of transgenic yeast for use in protein–protein interaction screening.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 Small-scale transformation of \u003ci\u003eS. cerevisiae.\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e3.2 Transformation of \u003ci\u003eS. cerevisiae\u003c\/i\u003e to test for non-specific interaction.\u003c\/p\u003e \u003cp\u003e3.3 Assaying for protein–protein interaction by reporter gene expression.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Yeast two-hybrid screening.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 Protein–protein interaction screening of a \u003ci\u003eC. elegans\u003c\/i\u003e cDNA library.\u003c\/p\u003e \u003cp\u003e4.2 Assaying for protein–protein interaction by reporter gene expression.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Isolation and identification of interacting proteins.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Preparation of electrocompetent \u003ci\u003eE. coli.\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e5.2 Isolation of DNA from yeast and electroporation of \u003ci\u003eE. coli.\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e5.3 Small-scale isolation of plasmid DNA from \u003ci\u003eE. coli\u003c\/i\u003e: the mini-prep.\u003c\/p\u003e \u003cp\u003e5.4 Sequencing of two-hybrid library plasmid DNA vectors.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Using bioinformatics in modern science.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e6.1 DNA sequence chromatogram.\u003c\/p\u003e \u003cp\u003e6.2 BLASTing your sequence.\u003c\/p\u003e \u003cp\u003e6.3 Evaluating sequence results and choosing an RNAi target.\u003c\/p\u003e \u003cp\u003e6.4 Bioinformatics practice questions.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Generation of an RNAi vector.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e7.1 Small-scale isolation of genomic DNA from \u003ci\u003eC. elegans.\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e7.2 PCR amplification of target gene sequence from \u003ci\u003eC. elegans\u003c\/i\u003e genomic DNA.\u003c\/p\u003e \u003cp\u003e7.3 Preparations for cloning to generate RNAi vector.\u003c\/p\u003e \u003cp\u003e7.3.1 Agarose gel electrophoresis.\u003c\/p\u003e \u003cp\u003e7.3.2 Removal of dNTPs from PCR reaction.\u003c\/p\u003e \u003cp\u003e7.3.3 Restriction enzyme digestion of PCR product and \u003ci\u003eC. elegans\u003c\/i\u003e RNAi vector.\u003c\/p\u003e \u003cp\u003e7.4 Gel purification of DNA and ligation of vector and PCR-amplified DNA.\u003c\/p\u003e \u003cp\u003e7.4.1 Preparative agarose gel electrophoresis.\u003c\/p\u003e \u003cp\u003e7.4.2 Gel purification of DNA from agarose gel.\u003c\/p\u003e \u003cp\u003e7.4.3 Ligation of vector and PCR-amplified DNA.\u003c\/p\u003e \u003cp\u003e7.5 Transformation of ligation reactions.\u003c\/p\u003e \u003cp\u003e7.6 PCR screening of transformation colonies.\u003c\/p\u003e \u003cp\u003e7.7 Small-scale isolation of plasmid DNA from \u003ci\u003eE. coli\u003c\/i\u003e: the mini-prep.\u003c\/p\u003e \u003cp\u003e7.8 Verifying successful ligation by restriction digestion.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 RNA-mediated interference by bacterial feeding.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8.1 Preparation of RNAi-feeding bacteria for transformation.\u003c\/p\u003e \u003cp\u003e8.2 Media preparation for RNAi feeding.\u003c\/p\u003e \u003cp\u003e8.3 Transformation of RNAi-feeding strain HT115(DE3).\u003c\/p\u003e \u003cp\u003e8.4 RNA interference by bacterial feeding of \u003ci\u003eC. elegans.\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e8.5 Analyzing effects of dsRNAi.\u003c\/p\u003e \u003cp\u003e8.5.1 Assaying for sterility (Ste) or embryonic lethality (Emb).\u003c\/p\u003e \u003cp\u003e8.5.2 Assaying for growth effect.\u003c\/p\u003e \u003cp\u003e8.5.3 Assaying for morphological effects.\u003c\/p\u003e \u003cp\u003e8.5.4 Assaying for general neuromuscular effects.\u003c\/p\u003e \u003cp\u003e8.5.5 Assaying for specific neuronal effects.\u003c\/p\u003e \u003cp\u003e8.5.6 Assaying for dauer formation.\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendix I Recombinational cloning.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eAI.1 Isolation of genomic DNA from \u003ci\u003eC. elegans.\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eAI.2 PCR amplification of target gene sequence from \u003ci\u003eC. elegans\u003c\/i\u003e genomic DNA.\u003c\/p\u003e \u003cp\u003eAI.3 Agarose gel electrophoresis and clean-up of PCR reaction.\u003c\/p\u003e \u003cp\u003eAI.4 Entry vector cloning.\u003c\/p\u003e \u003cp\u003eAI.5 Small-scale isolation of plasmid DNA from \u003ci\u003eE. coli\u003c\/i\u003e: the mini-prep.\u003c\/p\u003e \u003cp\u003eAI.6 Destination vector cloning.\u003c\/p\u003e \u003cp\u003eAI.7 Small-scale isolation of plasmid DNA from \u003ci\u003eE. coli\u003c\/i\u003e: the mini-prep.\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendix II Recipes and media preparation.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eSolution recipes.\u003c\/p\u003e \u003cp\u003eMedia preparation.\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendix III Sterile techniques and worm protocols.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eSterile techniques.\u003c\/p\u003e \u003cp\u003eWorm protocols.\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendix IV Mutant \u003ci\u003eC. elegans\u003c\/i\u003e phenotypes.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendix V Vector maps.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSubject index.\u003c\/b\u003e\u003c\/p\u003e","brand":"Wiley","offers":[{"title":"Default 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