{"product_id":"insect-histology-9781444336955","title":"Insect Histology","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003eThis title is a much needed update of Barbosa''s self-published \u003ci\u003eManual of Basic Techniques in Insect Histology\u003c\/i\u003e. It is a laboratory manual of ''traditional'' and ''modern'' insect histology techniques, completely revised using cutting-edge methodology carried out today and includes new immunohistochemical techniques not previously looked at. \u003cp\u003e\u003ci\u003eInsect Histology\u003c\/i\u003e is designed as a resource for student and professional researchers, in academia and industry, who require basic information on the procedures that are essential for the histological display of the tissues of insects and related organisms.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTrade Review\u003c\/b\u003e\u003cbr\u003e\u003cp\u003e“A very useful book to have in your library.”  (\u003ci\u003eBritish Journal of Entomology \u0026amp; Natural History\u003c\/i\u003e, 1 March 2015)\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003ePreface ix \u003cp\u003eAcknowledgements xi\u003c\/p\u003e \u003cp\u003eIntroduction xiii\u003c\/p\u003e \u003cp\u003eAbout the companion website xiv\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Problems of sclerotized chitin: Softening insect cuticle 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1.1 Introduction 1\u003c\/p\u003e \u003cp\u003e1.2 General Methods 3\u003c\/p\u003e \u003cp\u003e1.3 Preparations of insect eggs 14\u003c\/p\u003e \u003cp\u003e1.4 Double Embedding Techniques 16\u003c\/p\u003e \u003cp\u003eReferences 19\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Fixation 21\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 Introduction 21\u003c\/p\u003e \u003cp\u003e2.2 Aldehyde based fixatives 21\u003c\/p\u003e \u003cp\u003e2.3 Protein denaturing 30\u003c\/p\u003e \u003cp\u003e2.4 Picric acid based 33\u003c\/p\u003e \u003cp\u003e2.5 Mercuric chloride based 37\u003c\/p\u003e \u003cp\u003e2.6 SEM\/TEM 40\u003c\/p\u003e \u003cp\u003e2.7 Other 46\u003c\/p\u003e \u003cp\u003eReferences 51\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Dehydrating, clearing, and embedding 54\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 Dehydration 54\u003c\/p\u003e \u003cp\u003e3.2 Clearing 60\u003c\/p\u003e \u003cp\u003e3.3 Embedding General 65\u003c\/p\u003e \u003cp\u003e3.4 Embedding – Ester Wax 73\u003c\/p\u003e \u003cp\u003e3.5 Embedding – Methacrylate 74\u003c\/p\u003e \u003cp\u003eReferences 77\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Staining 79\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 Single-contrast staining – Carmines 81\u003c\/p\u003e \u003cp\u003e4.2 Single contrast staining – Nuclear Stains 83\u003c\/p\u003e \u003cp\u003e4.3 Single contrast staining – General Stains 86\u003c\/p\u003e \u003cp\u003e4.4 Single contrast staining – Golgi 89\u003c\/p\u003e \u003cp\u003e4.5 Single contrast staining – Eggs 89\u003c\/p\u003e \u003cp\u003e4.6 Single contrast staining – Silver Stains 90\u003c\/p\u003e \u003cp\u003e4.7 Polychrome staining techniques – General 92\u003c\/p\u003e \u003cp\u003e4.8 Polychrome staining – Brain\/Nerve 102\u003c\/p\u003e \u003cp\u003e4.9 Polychrome staining – blood 103\u003c\/p\u003e \u003cp\u003e4.10 Single contrast procedures for chitinous material 105\u003c\/p\u003e \u003cp\u003e4.11 Polychrome staining procedures for chitinous material 106\u003c\/p\u003e \u003cp\u003e4.12 Polychrome staining for chitinous material – KOH 110\u003c\/p\u003e \u003cp\u003e4.13 Polychrome staining for chitinous material – Differential staining of Individual Organs 111\u003c\/p\u003e \u003cp\u003e4.14 Staining of specific tissues 113\u003c\/p\u003e \u003cp\u003e4.15 Two dye combinations 114\u003c\/p\u003e \u003cp\u003eReferences 117\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Immunohistochemical techniques 119\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Introduction 119\u003c\/p\u003e \u003cp\u003e5.2 General immunostaining techniques 127\u003c\/p\u003e \u003cp\u003e5.3 Immunolabeling of samples for Transmission Electron Microscopy (TEM) 135\u003c\/p\u003e \u003cp\u003e5.4 Proliferation assays 140\u003c\/p\u003e \u003cp\u003e5.5 Methods to detect specific proteins 142\u003c\/p\u003e \u003cp\u003eReferences 144\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Use of genetic markers in insect histology 146\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e6.1 Introduction 146\u003c\/p\u003e \u003cp\u003e6.2 Inducible genetic markers 149\u003c\/p\u003e \u003cp\u003e6.3 Mosaic gene expression 156\u003c\/p\u003e \u003cp\u003e6.4 Fluorescent markers for live imaging and kinetic microscopy 165\u003c\/p\u003e \u003cp\u003eReferences 169\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Fluorescence 171\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction 171\u003c\/p\u003e \u003cp\u003eReferences 192\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Mounting 194\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8.1 Introduction 194\u003c\/p\u003e \u003cp\u003eReferences 206\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Preparation of whole mounts 208\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e9.1 Introduction 208\u003c\/p\u003e \u003cp\u003eReferences 229\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Preparation of whole mounts for staining 231\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e10.1 Introduction 231\u003c\/p\u003e \u003cp\u003e10.2 Detection of NAPDHd 237\u003c\/p\u003e \u003cp\u003e10.3 SEM 238\u003c\/p\u003e \u003cp\u003e10.4 In situ hybridization 240\u003c\/p\u003e \u003cp\u003eReferences 244\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Preparation of genitalia, mouthparts and other body parts 246\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eReferences 256\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Preparation of chromosomes 258\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eReferences 288\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Preparation of other specific insect organs and tissues 290\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e13.1 Introduction 290\u003c\/p\u003e \u003cp\u003eReferences 323\u003c\/p\u003e \u003cp\u003eAppendix Dissecting fluids and saline solutions 325\u003c\/p\u003e \u003cp\u003eIndex 333\u003c\/p\u003e","brand":"John Wiley and Sons Ltd","offers":[{"title":"Default Title","offer_id":49530087178583,"sku":"9781444336955","price":108.95,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9781444336955.jpg?v=1731878020","url":"https:\/\/bookcurl.com\/products\/insect-histology-9781444336955","provider":"Book Curl","version":"1.0","type":"link"}