{"product_id":"inorganic-chemical-biology-9781118510025","title":"Inorganic Chemical Biology","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003e\u003cp\u003eUnderstanding, identifying and influencing the biological systems are the primary objectives of\u003cbr\u003e chemical biology. From this perspective, metal complexes have always been of great assistance\u003cbr\u003e to chemical biologists, for example, in structural identification and purification of essential\u003cbr\u003e biomolecules, for visualizing cellular organelles or to inhibit specific enzymes. This inorganic side\u003cbr\u003e of chemical biology, which continues to receive considerable attention, is referred to as inorganic\u003cbr\u003e chemical biology.\u003c\/p\u003e \u003cp\u003e\u003ci\u003eInorganic Chemical Biology: Principles, Techniques and Applications\u003c\/i\u003e provides a comprehensive\u003cbr\u003e overview of the current and emerging role of metal complexes in chemical biology. Throughout all\u003cbr\u003e of the chapters there is a strong emphasis on fundamental theoretical chemistry and experiments\u003cbr\u003e that have been carried out in living cells or organisms. Outlooks for the future applications of\u003cbr\u003e metal complexes in chemical biology are \u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eAbout the Editor xiii\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eList of Contributors xv\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003ePreface xix\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eAcknowledgements xxi\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1. New Applications of Immobilized Metal Ion Affinity Chromatography in Chemical Biology 1\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eRachel Codd, Jiesi Gu, Najwa Ejje and Tulip Lifa\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e1.1 Introduction 1\u003c\/p\u003e \u003cp\u003e1.2 Principles and Traditional Use 2\u003c\/p\u003e \u003cp\u003e1.3 A Brief History 4\u003c\/p\u003e \u003cp\u003e1.4 New Application 1: Non-protein Based Low Molecular Weight Compounds 5\u003c\/p\u003e \u003cp\u003e1.4.1 Siderophores 6\u003c\/p\u003e \u003cp\u003e1.4.2 Anticancer Agent: Trichostatin A 10\u003c\/p\u003e \u003cp\u003e1.4.3 Anticancer Agent: Bleomycin 12\u003c\/p\u003e \u003cp\u003e1.4.4 Anti-infective Agents 13\u003c\/p\u003e \u003cp\u003e1.4.5 Other Agents 14\u003c\/p\u003e \u003cp\u003e1.4.6 Selecting a Viable Target 15\u003c\/p\u003e \u003cp\u003e1.5 New Application 2: Multi-dimensional Immobilized Metal Ion Affinity Chromatography 17\u003c\/p\u003e \u003cp\u003e1.6 New Application 3: Metabolomics 20\u003c\/p\u003e \u003cp\u003e1.7 New Application 4: Coordinate-bond Dependent Solid-phase Organic Synthesis 20\u003c\/p\u003e \u003cp\u003e1.8 Green Chemistry Technology 21\u003c\/p\u003e \u003cp\u003e1.9 Conclusion 23\u003c\/p\u003e \u003cp\u003eAcknowledgments 24\u003c\/p\u003e \u003cp\u003eReferences 24\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2. Metal Complexes as Tools for Structural Biology 37\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eMichael D. Lee, Bim Graham and James D. Swarbrick\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e2.1 Structural Biological Studies and the Major Techniques Employed 37\u003c\/p\u003e \u003cp\u003e2.2 What do Metal Complexes have to Offer the Field of Structural Biology? 38\u003c\/p\u003e \u003cp\u003e2.3 Metal Complexes for Phasing in X-ray Crystallography 39\u003c\/p\u003e \u003cp\u003e2.4 Metal Complexes for Derivation of Structural Restraints via Paramagnetic NMR Spectroscopy 41\u003c\/p\u003e \u003cp\u003e2.4.1 Paramagnetic Relaxation Enhancement (PRE) 42\u003c\/p\u003e \u003cp\u003e2.4.2 Residual Dipolar Coupling (RDC) 43\u003c\/p\u003e \u003cp\u003e2.4.3 Pseudo-Contact Shifts (PCS) 43\u003c\/p\u003e \u003cp\u003e2.4.4 Strategies for Introducing Lanthanide Ions into Bio-Macromolecules 44\u003c\/p\u003e \u003cp\u003e2.5 Metal Complexes as Spin Labels for Distance Measurements via EPR Spectroscopy 53\u003c\/p\u003e \u003cp\u003e2.6 Metal Complexes as Donors for Distance Measurements via Luminescence Resonance Energy Transfer (LRET) 54\u003c\/p\u003e \u003cp\u003e2.7 Concluding Statements and Future Outlook 56\u003c\/p\u003e \u003cp\u003eReferences 56\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3. AAS, XRF, and MS Methods in Chemical Biology of Metal Complexes 63\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eIngo Ott, Christophe Biot and Christian Hartinger\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e3.1 Introduction 63\u003c\/p\u003e \u003cp\u003e3.2 Atomic Absorption Spectroscopy (AAS) 64\u003c\/p\u003e \u003cp\u003e3.2.1 Fundamentals and Basic Principles of AAS 64\u003c\/p\u003e \u003cp\u003e3.2.2 Instrumental and Technical Aspects of AAS 65\u003c\/p\u003e \u003cp\u003e3.2.3 Method Development and Aspects of Practical Application 67\u003c\/p\u003e \u003cp\u003e3.2.4 Selected Application Examples 69\u003c\/p\u003e \u003cp\u003e3.3 Total Reflection X-Ray Fluorescence Spectroscopy (TXRF) 72\u003c\/p\u003e \u003cp\u003e3.3.1 Fundamentals and Basic Principles of TXRF 72\u003c\/p\u003e \u003cp\u003e3.3.2 Instrumental\/Methodical Aspects of TXRF and Applications 73\u003c\/p\u003e \u003cp\u003e3.4 Subcellular X-ray Fluorescence Imaging of a Ruthenium Analogue of the Malaria Drug Candidate Ferroquine Using Synchrotron Radiation 74\u003c\/p\u003e \u003cp\u003e3.4.1 Application of X-ray Fluorescence in Drug Development Using Ferroquine as an Example 75\u003c\/p\u003e \u003cp\u003e3.5 Mass Spectrometric Methods in Inorganic Chemical Biology 80\u003c\/p\u003e \u003cp\u003e3.5.1 Mass Spectrometry and Inorganic Chemical Biology: Selected Applications 83\u003c\/p\u003e \u003cp\u003e3.6 Conclusions 90\u003c\/p\u003e \u003cp\u003eAcknowledgements 90\u003c\/p\u003e \u003cp\u003eReferences 90\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4. Metal Complexes for Cell and Organism Imaging 99\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eKenneth Yin Zhang and Kenneth Kam-Wing Lo\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e4.1 Introduction 99\u003c\/p\u003e \u003cp\u003e4.2 Photophysical Properties 100\u003c\/p\u003e \u003cp\u003e4.2.1 Fluorescence and Phosphorescence 100\u003c\/p\u003e \u003cp\u003e4.2.2 Two-photon Absorption 101\u003c\/p\u003e \u003cp\u003e4.2.3 Upconversion Luminescence 102\u003c\/p\u003e \u003cp\u003e4.3 Detection of Luminescent Metal Complexes in an Intracellular Environment 104\u003c\/p\u003e \u003cp\u003e4.3.1 Confocal Laser-scanning Microscopy 104\u003c\/p\u003e \u003cp\u003e4.3.2 Fluorescence Lifetime Imaging Microscopy 105\u003c\/p\u003e \u003cp\u003e4.3.3 Flow Cytometry 106\u003c\/p\u003e \u003cp\u003e4.4 Cell and Organism Imaging 107\u003c\/p\u003e \u003cp\u003e4.4.1 Factors Affecting Cellular Uptake 107\u003c\/p\u003e \u003cp\u003e4.4.2 Organelle Imaging 116\u003c\/p\u003e \u003cp\u003e4.4.3 Two-photon and Upconversion Emission Imaging for Cells and Organisms 133\u003c\/p\u003e \u003cp\u003e4.4.4 Intracellular Sensing and Labeling 136\u003c\/p\u003e \u003cp\u003e4.5 Conclusion 143\u003c\/p\u003e \u003cp\u003eAcknowledgements 143\u003c\/p\u003e \u003cp\u003eReferences 143\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5. Cellular Imaging with Metal Carbonyl Complexes 149\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eLuca Quaroni and Fabio Zobi\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e5.1 Introduction 149\u003c\/p\u003e \u003cp\u003e5.2 Vibrational Spectroscopy of Metal Carbonyl Complexes 151\u003c\/p\u003e \u003cp\u003e5.3 Microscopy and Imaging of Cellular Systems 154\u003c\/p\u003e \u003cp\u003e5.3.1 Techniques of Vibrational Microscopy 155\u003c\/p\u003e \u003cp\u003e5.4 Infrared Microscopy 155\u003c\/p\u003e \u003cp\u003e5.4.1 Concentration Measurements with IR Spectroscopy and Spectromicroscopy 157\u003c\/p\u003e \u003cp\u003e5.4.2 Water Absorption 158\u003c\/p\u003e \u003cp\u003e5.4.3 Metal Carbonyls as IR Probes for Cellular Imaging 158\u003c\/p\u003e \u003cp\u003e5.4.4 In Vivo Uptake and Reactivity of Metal Carbonyl Complexes 162\u003c\/p\u003e \u003cp\u003e5.5 Raman Microscopy 167\u003c\/p\u003e \u003cp\u003e5.5.1 Concentration Measurements with Raman Spectroscopy and Spectromicroscopy 169\u003c\/p\u003e \u003cp\u003e5.5.2 Metal Carbonyls as Raman Probes for Cellular Imaging 169\u003c\/p\u003e \u003cp\u003e5.6 Near-field Techniques 171\u003c\/p\u003e \u003cp\u003e5.6.1 Concentration Measurements with Near-field Techniques 172\u003c\/p\u003e \u003cp\u003e5.6.2 High-resolution Measurement of Intracellular Metal–Carbonyl Accumulation by Photothermal Induced Resonance 173\u003c\/p\u003e \u003cp\u003e5.7 Comparison of Techniques 175\u003c\/p\u003e \u003cp\u003e5.8 Conclusions and Outlook 176\u003c\/p\u003e \u003cp\u003eAcknowledgements 177\u003c\/p\u003e \u003cp\u003eReferences 178\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6. Probing DNA Using Metal Complexes 183\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eLionel Marcélis, Willem Vanderlinden and Andrée Kirsch-De Mesmaeker\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e6.1 General Introduction 183\u003c\/p\u003e \u003cp\u003e6.2 Photophysics of Ru(II) Complexes 184\u003c\/p\u003e \u003cp\u003e6.2.1 The First Ru(II) Complex Studied in the Literature: [Ru(bpy)3]2+ 184\u003c\/p\u003e \u003cp\u003e6.2.2 Homoleptic Complexes 186\u003c\/p\u003e \u003cp\u003e6.2.3 Heteroleptic Complexes 186\u003c\/p\u003e \u003cp\u003e6.2.4 Photoinduced Electron Transfer (PET) and Energy Transfer Processes 188\u003c\/p\u003e \u003cp\u003e6.3 State-of-the-art on the Interactions of Mononuclear Ru(II) Complexes with Simple Double-stranded DNA 190\u003c\/p\u003e \u003cp\u003e6.3.1 Studies on Simple Double-stranded DNAs 191\u003c\/p\u003e \u003cp\u003e6.3.2 Influence of DNA on the Emission Properties 193\u003c\/p\u003e \u003cp\u003e6.4 Structural Diversity of the Genetic Material 194\u003c\/p\u003e \u003cp\u003e6.4.1 Mechanical Properties of DNA 195\u003c\/p\u003e \u003cp\u003e6.4.2 DNA Topology 195\u003c\/p\u003e \u003cp\u003e6.4.3 SMF Study with [Ru(phen)2(PHEHAT)]2+ and [Ru(TAP)2(PHEHAT)]2+ 198\u003c\/p\u003e \u003cp\u003e6.5 Unusual Interaction of Dinuclear Ru(II) Complexes with Different DNA Types 200\u003c\/p\u003e \u003cp\u003e6.5.1 Reversible Interaction of [{(Ru(phen)2}2HAT]4+ with Denatured DNA 201\u003c\/p\u003e \u003cp\u003e6.5.2 Targeting G-quadruplexes with Photoreactive [{Ru(TAP)2}2TPAC]4+ 204\u003c\/p\u003e \u003cp\u003e6.5.3 Threading Intercalation 205\u003c\/p\u003e \u003cp\u003e6.6 Conclusions 207\u003c\/p\u003e \u003cp\u003eAcknowledgement 208\u003c\/p\u003e \u003cp\u003eReferences 208\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7. Visualization of Proteins and Cells Using Dithiol-reactive Metal Complexes 215\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eDanielle Park, Ivan Ho Shon, Minh Hua, Vivien M. Chen and Philip J. Hogg\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e7.1 The Chemistry of As(III) and Sb(III) 215\u003c\/p\u003e \u003cp\u003e7.2 Cysteine Dithiols in Protein Function 217\u003c\/p\u003e \u003cp\u003e7.3 Visualization of Dithiols in Isolated Proteins with As(III) 218\u003c\/p\u003e \u003cp\u003e7.4 Visualization of Dithiols on the Mammalian Cell Surface with As(III) 218\u003c\/p\u003e \u003cp\u003e7.5 Visualization of Dithiols in Intracellular Proteins with As(III) 219\u003c\/p\u003e \u003cp\u003e7.6 Visualization of Tetracysteine-tagged Recombinant Proteins in Cells with As(III) 219\u003c\/p\u003e \u003cp\u003e7.7 Visualization of Cell Death in the Mouse with Optically Labelled As(III) 220\u003c\/p\u003e \u003cp\u003e7.7.1 Cell Death in Health and Disease 220\u003c\/p\u003e \u003cp\u003e7.7.2 Cell Death Imaging Agents 222\u003c\/p\u003e \u003cp\u003e7.7.3 Visualization of Cell Death in Mouse Tumours, Brain and Thrombi with Optically Labelled As(III) 223\u003c\/p\u003e \u003cp\u003e7.8 Visualization of Cell Death in Mouse Tumours with Radio-labelled As(III) 225\u003c\/p\u003e \u003cp\u003e7.9 Summary and Perspectives 227\u003c\/p\u003e \u003cp\u003eReferences 227\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8. Detection of Metal Ions, Anions and Small Molecules Using Metal Complexes 233\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eQin Wang and Katherine J. Franz\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e8.1 How Do We See What’s in a Cell? 233\u003c\/p\u003e \u003cp\u003e8.1.1 Why Metal Complexes as Sensors? 234\u003c\/p\u003e \u003cp\u003e8.1.2 Design Strategies for Sensors Built with Metal Complexes 234\u003c\/p\u003e \u003cp\u003e8.1.3 General Criteria of Metal-based Sensors for Bioimaging 236\u003c\/p\u003e \u003cp\u003e8.2 Metal Complexes for Detection of Metal Ions 236\u003c\/p\u003e \u003cp\u003e8.2.1 Tethered Sensors for Detecting Metal Ions 237\u003c\/p\u003e \u003cp\u003e8.2.2 Displacement Sensors for Detecting Metal Ions 240\u003c\/p\u003e \u003cp\u003e8.2.3 MRI Contrast Agents for Detecting Metal Ions 240\u003c\/p\u003e \u003cp\u003e8.2.4 Chemodosimeters for Metal Ions 249\u003c\/p\u003e \u003cp\u003e8.3 Metal Complexes for Detection of Anions and Neutral Molecules 252\u003c\/p\u003e \u003cp\u003e8.3.1 Tethered Approach: Metal Complex as Recognition Unit 255\u003c\/p\u003e \u003cp\u003e8.3.2 Displacement Approach: Metal Complex as Quencher 258\u003c\/p\u003e \u003cp\u003e8.3.3 Dosimeter Approach 262\u003c\/p\u003e \u003cp\u003e8.4 Conclusions 268\u003c\/p\u003e \u003cp\u003eAcknowledgements 268\u003c\/p\u003e \u003cp\u003eAbbreviations 268\u003c\/p\u003e \u003cp\u003eReferences 269\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9. Photo-release of Metal Ions in Living Cells 275\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eCelina Gwizdala and Shawn C. Burdette\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e9.1 Introduction to Photochemical Tools Including Photocaged Complexes 275\u003c\/p\u003e \u003cp\u003e9.2 Calcium Biochemistry and Photocaged Complexes 278\u003c\/p\u003e \u003cp\u003e9.2.1 Strategies for Designing Photocaged Complexes for Ca2+ 278\u003c\/p\u003e \u003cp\u003e9.2.2 Biological Applications of Photocaged Ca2+ Complexes 282\u003c\/p\u003e \u003cp\u003e9.3 Zinc Biochemistry and Photocaged Complexes 284\u003c\/p\u003e \u003cp\u003e9.3.1 Biochemical Targets for Photocaged Zn2+ Complexes 284\u003c\/p\u003e \u003cp\u003e9.3.2 Strategies for Designing Photocaged Complexes for Zn2+ 286\u003c\/p\u003e \u003cp\u003e9.4 Photocaged Complexes for Other Metal Ions 291\u003c\/p\u003e \u003cp\u003e9.4.1 Photocaged Complexes for Copper 291\u003c\/p\u003e \u003cp\u003e9.4.2 Photocaged Complexes for Iron 295\u003c\/p\u003e \u003cp\u003e9.4.3 Photocaged Complexes for Other Metal Ions 297\u003c\/p\u003e \u003cp\u003e9.5 Conclusions 298\u003c\/p\u003e \u003cp\u003eAcknowledgment 298\u003c\/p\u003e \u003cp\u003eReferences 298\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10. Release of Bioactive Molecules Using Metal Complexes 309\u003c\/b\u003e\u003cbr\u003e \u003ci\u003ePeter V. Simpson and Ulrich Schatzschneider\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e10.1 Introduction 309\u003c\/p\u003e \u003cp\u003e10.2 Small-molecule Messengers 310\u003c\/p\u003e \u003cp\u003e10.2.1 Biological Generation and Delivery of CO, NO, and H2S 310\u003c\/p\u003e \u003cp\u003e10.2.2 Metal–Nitrosyl Complexes for the Cellular Delivery of Nitric Oxide 311\u003c\/p\u003e \u003cp\u003e10.2.3 CO-releasing Molecules (CORMs) 314\u003c\/p\u003e \u003cp\u003e10.3 “Photouncaging” of Neurotransmitters from Metal Complexes 321\u003c\/p\u003e \u003cp\u003e10.3.1 “Caged” Compounds 321\u003c\/p\u003e \u003cp\u003e10.3.2 “Uncaging” of Bioactive Molecules 322\u003c\/p\u003e \u003cp\u003e10.4 Hypoxia Activated Cobalt Complexes 324\u003c\/p\u003e \u003cp\u003e10.4.1 Bioreductive Activation of Cobalt Complexes 324\u003c\/p\u003e \u003cp\u003e10.4.2 Hypoxia-activated Cobalt Prodrugs of DNA Alkylators 326\u003c\/p\u003e \u003cp\u003e10.4.3 Hypoxia-activated Cobalt Prodrugs of MMP Inhibitors 329\u003c\/p\u003e \u003cp\u003e10.5 Summary 333\u003c\/p\u003e \u003cp\u003eAcknowledgments 333\u003c\/p\u003e \u003cp\u003eReferences 323\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11. Metal Complexes as Enzyme Inhibitors and Catalysts in Living Cells 341\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eJulien Furrer, Gregory S. Smith and Bruno Therrien\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e11.1 Introduction 341\u003c\/p\u003e \u003cp\u003e11.2 Metal-based Inhibitors: From Serendipity to Rational Design 342\u003c\/p\u003e \u003cp\u003e11.2.1 Mimicking the Structure of Known Enzyme Binders 342\u003c\/p\u003e \u003cp\u003e11.2.2 Coordinating Known Enzymatic Inhibitors to Metal Complexes 343\u003c\/p\u003e \u003cp\u003e11.2.3 Exchanging Ligands to Inhibit Enzymes 344\u003c\/p\u003e \u003cp\u003e11.2.4 Controlling Conformation by Metal Coordination 344\u003c\/p\u003e \u003cp\u003e11.2.5 Competing with Known Metallo-Enzymatic Processes 345\u003c\/p\u003e \u003cp\u003e11.3 The Next Generation: Polynuclear Metal Complexes as Enzyme Inhibitors 346\u003c\/p\u003e \u003cp\u003e11.3.1 Polyoxometalates: Broad Spectrum Enzymatic Inhibitory Effects 347\u003c\/p\u003e \u003cp\u003e11.3.2 Polynuclear G-quadruplex DNA Stabilizers: Potential Inhibitors of Telomerase 349\u003c\/p\u003e \u003cp\u003e11.3.3 Polynuclear Polypyridyl Ruthenium Complexes: DNA Topoisomerase II Inhibitors 352\u003c\/p\u003e \u003cp\u003e11.4 Metal Complexes as Catalysts in Living Cells 355\u003c\/p\u003e \u003cp\u003e11.4.1 Catalysis of NAD+\/NADH 355\u003c\/p\u003e \u003cp\u003e11.4.2 Oxidation of the Thiols Cysteine and Glutathione 357\u003c\/p\u003e \u003cp\u003e11.4.3 Cytotoxicity Controlled by Oxidation 361\u003c\/p\u003e \u003cp\u003e11.5 Catalytic Conversion and Removal of Functional Groups 361\u003c\/p\u003e \u003cp\u003e11.6 Catalytically Controlled Carbon–Carbon Bond Formation 362\u003c\/p\u003e \u003cp\u003e11.7 Conclusion 364\u003c\/p\u003e \u003cp\u003eReferences 364\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12. Other Applications of Metal Complexes in Chemical Biology 373\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eTanmaya Joshi, Malay Patra and Gilles Gasser\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e12.1 Introduction 373\u003c\/p\u003e \u003cp\u003e12.2 Surface Immobilization of Proteins and Enzymes 373\u003c\/p\u003e \u003cp\u003e12.3 Metal Complexes as Artificial Nucleases 378\u003c\/p\u003e \u003cp\u003e12.3.1 Mono- and Multinuclear Cu(II) and Zn(II) Complexes 380\u003c\/p\u003e \u003cp\u003e12.3.2 Lanthanide Complexes 388\u003c\/p\u003e \u003cp\u003e12.4 Cellular Uptake Enhancement Using Metal Complexes 390\u003c\/p\u003e \u003cp\u003e12.5 Conclusions 394\u003c\/p\u003e \u003cp\u003e\u003ci\u003eAcknowledgments 394\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eReferences 394\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eIndex 403\u003c\/i\u003e\u003c\/p\u003e","brand":"John Wiley \u0026 Sons Inc","offers":[{"title":"Default Title","offer_id":49406883168599,"sku":"9781118510025","price":114.26,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9781118510025.jpg?v=1730497436","url":"https:\/\/bookcurl.com\/products\/inorganic-chemical-biology-9781118510025","provider":"Book Curl","version":"1.0","type":"link"}