{"product_id":"innate-immune-activation-methods-and-protocols-1714-methods-in-molecular-biology-9781493975181","title":"Innate Immune Activation Methods and Protocols","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003eChapters detail the assessment of macrophage activation, measuring innate immune responses to bacterial viability, quantification of secreted proteins, reporter systems, protocols examining specific innate immune activation by TLRs, RLRs, cGAS, and inflammasomes.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cp\u003e1          Emerging Concepts in Innate Immunity\u003c\/p\u003e  \u003cp\u003eKarin Pelka and Dominic De Nardo\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e2          Bioinformatic Assessment of Macrophage Activation by the Innate Immune System\u003c\/p\u003e  \u003cp\u003eThomas Ulas, Joachim L. Schultze, and Marc Beyer\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e3          Generation of Genetic Knockouts in Myeloid Cell Lines using a Lentiviral CRISPR\/Cas9 System\u003c\/p\u003e  \u003cp\u003ePaul J. Baker and Seth L. Masters\u003c\/p\u003e  \u003cp\u003e            \u003c\/p\u003e  \u003cp\u003e4          Modeling Primary Human Monocytes with the Trans-differentiation Cell Line BLaER1\u003c\/p\u003e  \u003cp\u003eMoritz M. Gaidt, Francesca Rapino, Thomas Graf, and Veit Hornung\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e5          Measurement of NF-κB Activation in TLR-activated Macrophages\u003c\/p\u003e  \u003cp\u003eOrna Ernst, Sharat J. Vayttaden, and Iain D.C. Fraser\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e6          Biochemical Isolation of the Myddosome from Murine Macrophages\u003c\/p\u003e  \u003cp\u003eYunhao Tan and Jonathan C. Kagan\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e7          Generation of Innate Immune Reporter Cell Lines using Retroviral Transduction\u003c\/p\u003e  \u003cp\u003eYamel Cardona Gloria and Dominic De Nardo\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e8          Myddosome Formation by Luminescence-based Mammalian Interactome Mapping (LUMIER)\u003c\/p\u003e  \u003cp\u003eOlaf-Oliver Wolz, Manfred Koegl, and Alexander N. R. Weber\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e9          Inflammatory Caspases: Activation and Cleavage of Gasdermin-D in vitro and during Pyroptosis\u003c\/p\u003e  \u003cp\u003eYue Zhao, Jianjin Shi and Feng Shao\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e10        Methods for Detecting ASC and ASC Speck Formation\u003c\/p\u003e  \u003cp\u003eFlorian Hoss, Verena Rolfes, Mariana R Davanso, Tarcio T Braga, and Bernardo S Franklin\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e11        Measuring Innate Immune Responses to Bacterial Viability\u003c\/p\u003e  \u003cp\u003eJulien Moretti, Nicolas Vabret, and J. Magarian Blander\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e12        Methods to Study Cell Swelling-induced Inflammasome Activation\u003c\/p\u003e  \u003cp\u003e            Vincent Compan and Pablo Pelegrín\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e13        Detecting Release of Bacterial dsDNA into the Host Cytosol using Fluorescence Microscopy\u003c\/p\u003e  \u003cp\u003eRoland Felix Dreier, José Carlos Santos, and Petr Broz        \u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e14        Quantitative Proteomics of Secreted Proteins\u003c\/p\u003e  \u003cp\u003eAnnika Frauenstein and Felix Meissner\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e15        Simultaneous Detection of Cellular Viability and Interleukin-1β Secretion from Single Cells by ELISpot \u003c\/p\u003e  \u003cp\u003eStephanie A. Conos, Lisa M. Lindqvist, and James E. Vince\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e16        Detection and Quantification of MAV Aggregation via Confocal Imaging\u003c\/p\u003e  \u003cp\u003eTan A Nguyen, Lachlan Whitehead, and Ken C Pang\u003c\/p\u003e","brand":"Humana Press Inc.","offers":[{"title":"Default Title","offer_id":49409211105623,"sku":"9781493975181","price":104.99,"currency_code":"GBP","in_stock":false}],"url":"https:\/\/bookcurl.com\/products\/innate-immune-activation-methods-and-protocols-1714-methods-in-molecular-biology-9781493975181","provider":"Book Curl","version":"1.0","type":"link"}