{"product_id":"in-vitro-bioassay-techniques-for-anticancer-drug-discovery-and-development-9781138725829","title":"In Vitro Bioassay Techniques for Anticancer Drug","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003e\u003cp\u003eThis comprehensive and useful handbook represents a definitive up-to-date compendium of key in vitro bioassay methods that are employed to quantify and validate the anticancer activity of a drug candidate before it makes its way in to animal or clinical trials.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e\u003cem\u003eIn Vitro Bioassay Techniques for Anticancer Drug Discovery and Development\u003c\/em\u003e\u003c\/strong\u003e covers the screening and evaluation of potential drug candidates in a wide category of anticancer assays demonstrating the specific ways in which various pharmaceutical bioassays interpret the activity of drug molecules. The major emphasis of the book is to present those bioassays which can be readily set up and practiced in any laboratory with limited funds, facilities or technical know-how.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eIntroduction to Anticancer Drug Development\u003c\/strong\u003e. \u003c\/p\u003e\u003cp\u003eCancer. \u003c\/p\u003e\u003cp\u003eChemotherapy. \u003c\/p\u003e\u003cp\u003eNeed for Effective Anticancer Drugs. \u003c\/p\u003e\u003cp\u003eStages of Anticancer Drug Development. \u003c\/p\u003e\u003cp\u003eReferences. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eIn Vitro Biological Assays for Anticancer Chemotherapeutics. \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eIntroduction.\u003cstrong\u003e \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eAdvantages of In Vitro Assays. \u003c\/p\u003e\u003cp\u003eDisadvantages of In Vitro Assays. \u003c\/p\u003e\u003cp\u003eClassification of In Vitro Assays. \u003c\/p\u003e\u003cp\u003eEssential Techniques of Cell Cultures. \u003c\/p\u003e\u003cp\u003ePrimary Cell Cultures. \u003c\/p\u003e\u003cp\u003eMaintenance of Cell Lines. \u003c\/p\u003e\u003cp\u003eSubculture of Cells. \u003c\/p\u003e\u003cp\u003eReferences. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell Viability and Cytotoxicity Assays. \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eIntroduction. \u003c\/p\u003e\u003cp\u003eCell Membrane Integrity Assays. \u003c\/p\u003e\u003cp\u003eDye Exclusion Assays. \u003c\/p\u003e\u003cp\u003eLDH Release Assays. \u003c\/p\u003e\u003cp\u003eFunctional or Metabolic Assays. \u003c\/p\u003e\u003cp\u003eTetrazolium Reduction Assays. \u003c\/p\u003e\u003cp\u003eSulforhodamine B Assay. \u003c\/p\u003e\u003cp\u003eAcid Phosphatase Assay. \u003c\/p\u003e\u003cp\u003eAlamar Blue Oxidation-Reduction Assay. \u003c\/p\u003e\u003cp\u003eNeutral Red Assay. \u003c\/p\u003e\u003cp\u003eProtease Viability Marker Assay. \u003c\/p\u003e\u003cp\u003eCell Adhesion Assay. \u003c\/p\u003e\u003cp\u003eReferences. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell Proliferation Assays. \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eIntroduction. \u003c\/p\u003e\u003cp\u003eDNA Labeling Assays. \u003c\/p\u003e\u003cp\u003e[3H]-Thymidine Assay. \u003c\/p\u003e\u003cp\u003eBrdU Incorporation Assay. \u003c\/p\u003e\u003cp\u003eMetabolic Assays. \u003c\/p\u003e\u003cp\u003eDye Assays. \u003c\/p\u003e\u003cp\u003eATP Assays. \u003c\/p\u003e\u003cp\u003eClonogenic Reproductive Assay.\u003c\/p\u003e\u003cp\u003eReferences.\u003cstrong\u003e \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eApoptosis Assays. \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eIntroduction. \u003c\/p\u003e\u003cp\u003eLight Microscopy. \u003c\/p\u003e\u003cp\u003eWestern Blot Caspase Activation Assay. \u003c\/p\u003e\u003cp\u003eAnnexin V Ataining. \u003c\/p\u003e\u003cp\u003eDNA Ladder Assay. \u003c\/p\u003e\u003cp\u003eELISA. \u003c\/p\u003e\u003cp\u003eTUNEL Assay. \u003c\/p\u003e\u003cp\u003eComet Assay. \u003c\/p\u003e\u003cp\u003eAcridine Orange\/Ethidium Bromide Staining. \u003c\/p\u003e\u003cp\u003eMitochondrial Membrane Potential Assay. \u003c\/p\u003e\u003cp\u003eChromatin Condensation Assay. \u003c\/p\u003e\u003cp\u003eAPOPercentageTM Apoptotic Assay.\u003c\/p\u003e\u003cp\u003ePARP Cleavage Assay. \u003c\/p\u003e\u003cp\u003eFlow Cytometry. \u003c\/p\u003e\u003cp\u003eReferences. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCell Migratory Assays\u003c\/strong\u003e. \u003c\/p\u003e\u003cp\u003eIntroduction.\u003c\/p\u003e\u003cp\u003e2D Cell Migration Assays. \u003c\/p\u003e\u003cp\u003eWound Healing Assay. \u003c\/p\u003e\u003cp\u003eCell Exclusion Zone Assay. \u003c\/p\u003e\u003cp\u003eTranswell Migration Assay\u003cstrong\u003e.\u003c\/strong\u003e \u003c\/p\u003e\u003cp\u003eFence Assay. \u003c\/p\u003e\u003cp\u003eMicrofluidic Assays. \u003c\/p\u003e\u003cp\u003eSingle Cell Motility Assay. \u003c\/p\u003e\u003cp\u003e2D\/3D Migration Assays. \u003c\/p\u003e\u003cp\u003eMicrocarrier Bead Assay. \u003c\/p\u003e\u003cp\u003eSpheroid Migration Assay. \u003c\/p\u003e\u003cp\u003eReferences. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eIn Vitro Cell Invasion Assays\u003c\/strong\u003e. \u003c\/p\u003e\u003cp\u003eIntroduction. \u003c\/p\u003e\u003cp\u003eTranswell Invasion Assay. \u003c\/p\u003e\u003cp\u003ePlatypus Invasion Assay. \u003c\/p\u003e\u003cp\u003eGelatin Degradation Assay. \u003c\/p\u003e\u003cp\u003e3D Cell Tracking Assay. \u003c\/p\u003e\u003cp\u003eVertical Gel 3D Invasion Assay. \u003c\/p\u003e\u003cp\u003eSpheroid\/Monodispersed Cell Invasion Assay. \u003c\/p\u003e\u003cp\u003eSpheroid Confrontation Assay.\u003c\/p\u003e\u003cp\u003eSpheroid Gel Invasion Assays. \u003c\/p\u003e\u003cp\u003eReferences. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAnti-Angiogenesis Assays. \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eIntroduction. \u003c\/p\u003e\u003cp\u003eEndothelial Tube Formation Assay. \u003c\/p\u003e\u003cp\u003eRat Aortic Ring Assay. \u003c\/p\u003e\u003cp\u003eReferences.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eHigh-Throughput Screening Assays.\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eIntroduction.\u003c\/p\u003e\u003cp\u003eCell Cultures.\u003c\/p\u003e\u003cp\u003eHigh-Throughput Cytotoxicity and Apoptosis Screening in 2D Cultures.\u003c\/p\u003e\u003cp\u003e3D Cell-Based HTS Assays.\u003c\/p\u003e\u003cp\u003eReferences.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eIndex.\u003c\/strong\u003e\u003c\/p\u003e","brand":"Taylor \u0026 Francis Ltd","offers":[{"title":"Default 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