{"product_id":"human-stem-cell-technology-and-biology-9780470595459","title":"Human Stem Cell Technology and Biology","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003e\u003ci\u003eHuman Stem Cell Technology \u0026amp; Biology: A Research Guide and Laboratory Manual\u003c\/i\u003e integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells.  \u003cp\u003eKey Features:\u003c\/p\u003e \u003cul\u003e \u003cli\u003eAn authoritative, comprehensive, multimedia training manual for stem cell researchers\u003c\/li\u003e \u003cli\u003eEasy to follow step-by-step laboratory protocols and instructional videos provide a valuable resource\u003c\/li\u003e \u003cli\u003eA must-have for developing laboratory course curriculums, training courses, and workshops in stem cell biology\u003c\/li\u003e \u003cli\u003ePerspectives written by the world leaders in the field\u003c\/li\u003e \u003cli\u003eIntroductory chapters will provide background information\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThe volume will be a valuable reference resource for both experienced investigators pursuing stem cell and induced pluripotent stem cell research as well as those new to this field.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTrade Review\u003c\/b\u003e\u003cbr\u003e\"The topic is intrinsically fascinating and clinically relevant ... I recommend this book for novice and experienced scientists interested in stem cell biology. It will serve as an excellent reference manual and practical laboratory workbook.\" (Doody's, 17 February 2012)  \u003cp\u003e\"We would recommend this readable and well-organised book to all laboratories dealing with human ES cells ... This book will act as an excellent guide.\" (Development Journal, 1 December 2011)\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003eForeword.  \u003cp\u003ePreface.\u003c\/p\u003e \u003cp\u003eAcknowledgments.\u003c\/p\u003e \u003cp\u003eEditors and Contributors.\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSECTION I: INTRODUCTION.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1 Introduction to Pluripotent Stem Cells: Biology and Applications (\u003ci\u003eMaria Borowski and Gary S. Stein\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e2 Researching and Obtaining Established Stem Cell Lines (\u003ci\u003eMai X. Luong, Kelly P. Smith, and Gary S. Stein\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSECTION II: LABORATORY GUIDE FOR HUMAN STEM CELL CULTURE: PLURIPOTENT STEM CELL CULTURE.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3 Basics of Cell Culture (\u003ci\u003eAlicia Allaire, Mai X. Luong, and Kelly P. Smith\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e4 The Stem Cell Laboratory (\u003ci\u003eAlicia Allaire, Mai X. Luong, and Kelly P. Smith\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e5 Reagent Preparation (\u003ci\u003eAlicia Allaire, Mai X. Luong, and Kelly P. Smith\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e6 Preparation of Mouse Embryonic Fibroblasts for Culture of Human Embryonic Stem Cells (\u003ci\u003eMeng-Jiao Shi, Maria Borowski, and Kimberly Stencel\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e7 Thawing and Seeding of Frozen Mouse Embryonic Fibroblasts (\u003ci\u003eMeng-Jiao Shi, Maria Borowski, and Kimberly Stencel\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e8 Thawing, Seeding, and Changing the Medium of Human Embryonic Stem Cells (\u003ci\u003eMeng-Jiao Shi, Kimberly Stencel, and Maria Borowski\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e9 Passaging of Human Embryonic Stem Cells on Inactivated Mouse Embryonic Fibroblast Plates (\u003ci\u003eMeng-Jiao Shi, Kimberly Stencel, and Maria Borowski\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e10 Harvesting Human Embryonic Stem Cells for Cryopreservation (\u003ci\u003eMeng-Jiao Shi, Kimberly Stencel, and Maria Borowski\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e11 Human Embryonic Stem Cell Culture on BD Matrigel™with mTeSR®1 Medium (\u003ci\u003eMeng-Jiao Shi, Kimberly Stencel, and Maria Borowski\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSECTION III: LABORATORY GUIDE FOR HUMAN STEM CELL CULTURE: CHARACTERIZATION OF PLURIPOTENT STEM CELLS.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e12 Defining Pluripotency (\u003ci\u003eKelly P. Smith and Mai X. Luong\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e13 Characterization of Human Embryonic Stem Cells by Immunofluorescence Microscopy (\u003ci\u003eShirwin M. Pockwinse and Prachi N. Ghule\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e14 Preparation of Human Embryonic Stem Cell Samples for Flow Cytometry (\u003ci\u003eMeng-Jiao Shi, Kimberly Stencel, and Maria Borowski\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e15 Characterization of Human Embryonic Stem Cell Gene Expression Using Reverse Transcription Polymerase Chain Reaction (\u003ci\u003eAlicia Allaire, Kelly P. Smith, and Mai X. Luong\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e16 Determining Pluripotency of Human Embryonic Stem Cells: Embryoid Body Formation (\u003ci\u003eShirwin M. Pockwinse and Prachi N. Ghule\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e17 Characterization of Human Embryonic Stem Cells by Cytogenetics: Karyotyping and Fluorescence \u003ci\u003eIn Situ\u003c\/i\u003e Hybridization (\u003ci\u003eShirwin M. Pockwinse, Prachi N. Ghule and Anne Higgins\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e18 High-Resolution Chromatin Immunoprecipitation Assay (\u003ci\u003eBeatriz Perez-Cadahıa, Bojan Drobic, and James R. Davie\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e19 Assaying Pluripotency Via Teratoma Formation (\u003ci\u003eM. William Lensch and Tan A. Ince\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSECTION\u003c\/b\u003e \u003cb\u003eIV:\u003c\/b\u003e \u003cb\u003ePERSPECTIVES IN HUMAN STEM CELL TECHNOLOGIES.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e20 Genomic Analysis of Pluripotent Stem Cells (\u003ci\u003eDavid Lapointe\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e21 Proteomic Analysis of Human Pluripotent Cells (\u003ci\u003eAndy T. Y. Lau, Yan-Ming Xu, and Jen-Fu Chiu\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e22 Bioinformatics Strategies for Understanding Gene Expression in Human Pluripotent Cells (\u003ci\u003eGustavo Glusman, Bruz Marzolf, Kai Wang, Ji-Hoon Cho, Burak Kutlu, and Qiang Tian\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e23 Epigenetic Analysis of Pluripotent Cells (\u003ci\u003eMojgan Rastegar, Genevieve P. Delcuve, and James R. Davie\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e24 Differentiation of Pluripotent Stem Cells: An Overview (\u003ci\u003eJeremy Micah Crook\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e25 Cellular Reprogramming: Current Technology, Perspectives, and Generation of Induced Pluripotent Cells (\u003ci\u003eTanja Dominko\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSECTION V: APPLICATIONS OF HUMAN EMBRYONIC STEM CELLS.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e26 Human Pluripotent Cells: The Biology of Pluripotency (\u003ci\u003eLi-Fang Chu and Thomas P. Zwaka\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e27 Human Pluripotent Cells for Regenerative Medicine: Potential Applications for Regenerative Medicine (\u003ci\u003eChristopher C. Ford and Darrell N. Kotton\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e28 Therapeutic Applications of Human Embryonic Stem Cells (\u003ci\u003eShi-Jiang Lu, Irina Klimanskaya, Edmund Mickunas, and Robert Lanza\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e29 Human Stem Cells for Drug Screening, Toxicity, Specificity, and Off-Target Effects (\u003ci\u003eArnaud Lacoste and Mark Burcin\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e30 Interfacing Stem Cells with Gene Therapy (\u003ci\u003eChristian Mueller and Terence R. Flotte\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e31 Tissue Engineering for Stem Cell Mediated Regenerative Medicine (\u003ci\u003eJanet Zoldan, Thomas P. Kraehenbuehl, Abigail K. R. Lytton-Jean, Robert S. Langer, and Daniel G. Anderson\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e\u003cb\u003eGlossary.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eIndex.\u003c\/b\u003e\u003c\/p\u003e","brand":"John Wiley and Sons Ltd","offers":[{"title":"Default Title","offer_id":49525381529943,"sku":"9780470595459","price":139.6,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9780470595459.jpg?v=1731860299","url":"https:\/\/bookcurl.com\/products\/human-stem-cell-technology-and-biology-9780470595459","provider":"Book Curl","version":"1.0","type":"link"}