{"product_id":"human-pluripotent-stem-cells-9781119394334","title":"Human Pluripotent Stem Cells","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eComprehensive coverage of the entire induced pluripotent stem cell basic work flow\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003ePluripotent stem cells (PSC) can divide indefinitely, self-renew, and can differentiate to functionally reconstitute almost any cell in the normal developmental pathway, given the right conditions. This comprehensive book, which was developed from a training course, covers all of the PSCs (embryonic, embryonic germ, and embryonic carcinoma) and their functions. It demonstrates the feeder-dependent and feeder-free culture of hESC and hiPSC, which will be referred to in all protocols as PSCs. It also addresses the methods commonly used to determine pluripotency, as defined by self-renewal marker expression and differentiation potential.\u003c\/p\u003e \u003cp\u003e\u003ci\u003eHuman Pluripotent Stem Cells: A Practical Guide\u003c\/i\u003e offers in-depth chapter coverage of introduction to stem cell, PSC culture, reprogramming, differentiation, PSC characterization, and more. It also includes four appendixes containing information\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e1 Introduction 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1.1 Biosafety 1\u003c\/p\u003e \u003cp\u003e1.2 Biosafety Levels 2\u003c\/p\u003e \u003cp\u003e1.3 Aseptic Technique 2\u003c\/p\u003e \u003cp\u003e1.4 Storage 6\u003c\/p\u003e \u003cp\u003e1.5 Contamination 7\u003c\/p\u003e \u003cp\u003e1.6 Pluripotent Stem Cells 8\u003c\/p\u003e \u003cp\u003e1.7 Procedures 9\u003c\/p\u003e \u003cp\u003eReferences 16\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Pluripotent Stem Cell Culture 17\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 Introduction 17\u003c\/p\u003e \u003cp\u003e2.2 Materials 18\u003c\/p\u003e \u003cp\u003e2.3 Solutions 19\u003c\/p\u003e \u003cp\u003e2.4 Methods 21\u003c\/p\u003e \u003cp\u003eReferences 47\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Reprogramming 49\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 Introduction 49\u003c\/p\u003e \u003cp\u003e3.2 Materials 49\u003c\/p\u003e \u003cp\u003e3.3 Solutions 54\u003c\/p\u003e \u003cp\u003e3.4 Methods 57\u003c\/p\u003e \u003cp\u003eReferences 97\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Characterization 99\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 Introduction 99\u003c\/p\u003e \u003cp\u003e4.2 Materials 101\u003c\/p\u003e \u003cp\u003e4.3 Solutions 103\u003c\/p\u003e \u003cp\u003e4.4 Methods 104\u003c\/p\u003e \u003cp\u003eReferences 128\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Differentiation 131\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Introduction 131\u003c\/p\u003e \u003cp\u003e5.2 Materials 131\u003c\/p\u003e \u003cp\u003e5.3 Solutions 133\u003c\/p\u003e \u003cp\u003e5.4 Methods 135\u003c\/p\u003e \u003cp\u003eReferences 151\u003c\/p\u003e \u003cp\u003eIndex 153\u003c\/p\u003e","brand":"John Wiley and Sons Ltd","offers":[{"title":"Default Title","offer_id":49407045468503,"sku":"9781119394334","price":999.99,"currency_code":"GBP","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9781119394334.jpg?v=1730497987","url":"https:\/\/bookcurl.com\/products\/human-pluripotent-stem-cells-9781119394334","provider":"Book Curl","version":"1.0","type":"link"}