{"product_id":"fundamentals-of-contemporary-mass-spectrometry-16-wiley-series-on-mass-spectrometry-9780471682295","title":"Fundamentals of Contemporary Mass Spectrometry 16","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003eA concise and authoritative overview of mass spectrometry instrumentation, techniques, and applications in a very readable, user-friendly format filled with chapter summaries, exercises and answers, and references to useful outside resources. * Includes numerous exercises in every chapter, with the answers at the back of the book.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTrade Review\u003c\/b\u003e\u003cbr\u003e“It presents a well written, informative, and welcome addition to the selection of textbooks on the topic.” (\u003ci\u003eAnalytical Science and Bioanalytical Chemistry\u003c\/i\u003e, August 2007)  \u003cp\u003e\"The book will be a good teaching tool of the principles of mass spectrometry to undergraduates and graduates.\" (\u003ci\u003eInternational Journal of Environmental and Analytical Chemistry\u003c\/i\u003e, 2008)\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cb\u003ePART 1: INSTRUMENTATION.\u003c\/b\u003e  \u003cp\u003e \u003cb\u003e1. BASICS OF MASS SPECTROMETRY.\u003c\/b\u003e  \u003c\/p\u003e\u003cp\u003e 1.1. A BRIEF HISTORY OF MASS SPECTROMETRY.  \u003c\/p\u003e\u003cp\u003e 1.2. UNIQUE FEATURES OF MASS SPECTROMETRY.  \u003c\/p\u003e\u003cp\u003e 1.3. BASIC PRINCIPLES OF MASS SPECTROMETRY.  \u003c\/p\u003e\u003cp\u003e 1.4. ANATOMY OF A MASS SPECTRUM.  \u003c\/p\u003e\u003cp\u003e 1.5. ATOMIC AND MOLECULAR MASSES.  \u003c\/p\u003e\u003cp\u003e 1.5.1. Mass-to-charge Ratio.  \u003c\/p\u003e\u003cp\u003e 1.6. GENERAL APPLICATIONS.  \u003c\/p\u003e\u003cp\u003e 1.7. OVERVIEW OF THE CHAPTER.  \u003c\/p\u003e\u003cp\u003e 1.8. EXCERCISES.  \u003c\/p\u003e\u003cp\u003e 1.9. REFERENCES.  \u003c\/p\u003e\u003cp\u003e \u003cb\u003e2. MODES OF IONIZATION.\u003c\/b\u003e  \u003c\/p\u003e\u003cp\u003e 2.1. WHY IONIZATION IS REQUIRED?  \u003c\/p\u003e\u003cp\u003e 2.2. GENERAL CONSTRUCTION OF THE ION SOURCE.  \u003c\/p\u003e\u003cp\u003e GAS-PHASE IONIZATION TECHNIQUES.  \u003c\/p\u003e\u003cp\u003e 2.3. ELECTRON IONIZATION.  \u003c\/p\u003e\u003cp\u003e 2.4. CHEMICAL IONIZATION.  \u003c\/p\u003e\u003cp\u003e 2.4.1. Charge-exchange Chemical Ionization.  \u003c\/p\u003e\u003cp\u003e 2.4.2. Negative-Ion Chemical Ionization.  \u003c\/p\u003e\u003cp\u003e 2.5. PHOTOIONIZATION.  \u003c\/p\u003e\u003cp\u003e 2.6. FIELD IONIZTION.  \u003c\/p\u003e\u003cp\u003e 2.7. METASTABLE ATOM BOMBARDMENT IONIZATION.  \u003c\/p\u003e\u003cp\u003e \u003cb\u003eCONDENSED-PHASE IONIZATION TECHNIQUES: IONIZATION OF SOLID-STATE SAMPLES.\u003c\/b\u003e  \u003c\/p\u003e\u003cp\u003e 2.8. FIELD DESORPTION.  \u003c\/p\u003e\u003cp\u003e 2.9. PLASMA DESORPTION IONIZATION.  \u003c\/p\u003e\u003cp\u003e 2.10. SECONDARY-ION MASS SPECTROMETRY .  \u003c\/p\u003e\u003cp\u003e 2.11. FAST ATOM BOMBARDMENT.  \u003c\/p\u003e\u003cp\u003e 2.12. LASER DESORPTION\/IONIZATION.  \u003c\/p\u003e\u003cp\u003e 2.13. MATRIX-ASSISTED LASER DESORPTION\/IONIZATION.  \u003c\/p\u003e\u003cp\u003e 2.13.1. Analysis of Low Molecular Mass Compounds by MALDI .  \u003c\/p\u003e\u003cp\u003e 2.13.2. Atmospheric Pressure-MALDI.  \u003c\/p\u003e\u003cp\u003e 2.13.3. Surface-enhanced Laser Desorption\/Ionization.  \u003c\/p\u003e\u003cp\u003e \u003cb\u003eCONDENSED-PHASE IONIZATION TECHNIQUES: IONIZATION OF LIQUID-STATE SAMPLES .\u003c\/b\u003e  \u003c\/p\u003e\u003cp\u003e 2.14. THERMOSPRAY IONIZATION.  \u003c\/p\u003e\u003cp\u003e 2.15. ATMOSPHERIC PRESSURE CHEMICAL IONIZATION.  \u003c\/p\u003e\u003cp\u003e 2.16. ATMOSPHERIC PRESSURE PHOTOIONIZATION.  \u003c\/p\u003e\u003cp\u003e 2.17. ELECTROSPRAY IONIZATION.  \u003c\/p\u003e\u003cp\u003e 2.17.1. Mechanism of Electrospray Ionization .  \u003c\/p\u003e\u003cp\u003e 2.17.2. Sample Consideration .  \u003c\/p\u003e\u003cp\u003e 2.17.3. Nanoelectrospray Ionization .  \u003c\/p\u003e\u003cp\u003e 2.18. DESORPTION ELECTROSPRAY IONIZATION.  \u003c\/p\u003e\u003cp\u003e 2.18.1. DART Ion Source.  \u003c\/p\u003e\u003cp\u003e 2.19. OVERVIEW OF THE CHAPTER.  \u003c\/p\u003e\u003cp\u003e 2.19. EXERCISES.  \u003c\/p\u003e\u003cp\u003e 2.20. ADDITIONAL READING.  \u003c\/p\u003e\u003cp\u003e 2.21. REFERENCES.  \u003c\/p\u003e\u003cp\u003e \u003cb\u003e3. MASS ANALYSIS AND ION DETECTION.\u003c\/b\u003e  \u003c\/p\u003e\u003cp\u003e 3.1. MASS RESOLUTION.  \u003c\/p\u003e\u003cp\u003e 3.2. KINETIC ENERGY OF IONS.  \u003c\/p\u003e\u003cp\u003e MASS ANALYZERS.  \u003c\/p\u003e\u003cp\u003e 3.3. MAGNETIC SECTOR MASS SPECTROMETERS.  \u003c\/p\u003e\u003cp\u003e 3.3.1. Working Principle of a Magnetic Analyzer.  \u003c\/p\u003e\u003cp\u003e 3.3.2. Working Principle of an Electrostatic Analyzer.  \u003c\/p\u003e\u003cp\u003e 3.3.3. Working Principle of Double-Focusing Magnetic Sector Mass Spectrometers.  \u003c\/p\u003e\u003cp\u003e 3.3.4. Performance Characteristics .  \u003c\/p\u003e\u003cp\u003e 3.4. QUADRUPOLE MASS SPCETROMETERS.  \u003c\/p\u003e\u003cp\u003e 3.4.1. Working Principle.  \u003c\/p\u003e\u003cp\u003e 3.4.2. Performance Characteristics .  \u003c\/p\u003e\u003cp\u003e 3.4.3. RF-only quadrupole .  \u003c\/p\u003e\u003cp\u003e 3.5. TIME-OF-FLIGHT MASS SPECTROMETERS.  \u003c\/p\u003e\u003cp\u003e 3.5.1. Working Principle.  \u003c\/p\u003e\u003cp\u003e 3.5.2. Delayed Extraction of Ions .  \u003c\/p\u003e\u003cp\u003e 3.5.3. Reflectron TOF Instrument .  \u003c\/p\u003e\u003cp\u003e 3.5.4. Orthogonal Acceleration TOF Mass Spectrometer.  \u003c\/p\u003e\u003cp\u003e 3.5.5. Performance Characteristics.  \u003c\/p\u003e\u003cp\u003e 3.6. QUADRUPOLE ION TRAP MASS SPECTROMETERS.  \u003c\/p\u003e\u003cp\u003e 3.6.1. Working Principle.  \u003c\/p\u003e\u003cp\u003e 3.6.2. Operational Modes.  \u003c\/p\u003e\u003cp\u003e 3.6.3. Performance Characteristics .  \u003c\/p\u003e\u003cp\u003e 3.7. LINEAR ION TRAP MASS SPECTROMETERS.  \u003c\/p\u003e\u003cp\u003e 3.7.1. Rectilinear Ion Trap.  \u003c\/p\u003e\u003cp\u003e 3.8. FOURIER-TRANSFORM ION CYCLOTRON RESONANCE MASS SPECTROMETERS.  \u003c\/p\u003e\u003cp\u003e 3.8.1. Working Principle.  \u003c\/p\u003e\u003cp\u003e 3.8.2. Performance Characteristics.  \u003c\/p\u003e\u003cp\u003e 3.9. ORBITRAP MASS ANALYZER.  \u003c\/p\u003e\u003cp\u003e 3.10. ION MOBILITY MASS SPECTROMETERS.  \u003c\/p\u003e\u003cp\u003e 3.11. DETECTORS.  \u003c\/p\u003e\u003cp\u003e 3.11.1. Faraday Cup Detector.  \u003c\/p\u003e\u003cp\u003e 3.11.2. Electron Multipliers.  \u003c\/p\u003e\u003cp\u003e 3.11.3. Photomultiplier detectors.  \u003c\/p\u003e\u003cp\u003e 3.11.4. Post-acceleration Detectors.  \u003c\/p\u003e\u003cp\u003e 3.11.5. Low-temperature Calorimetric Detectors for High Mass Ions.  \u003c\/p\u003e\u003cp\u003e 3.11.6. Focal-plane Detectors.  \u003c\/p\u003e\u003cp\u003e 3.12. OVERVIEW OF THE CHAPTER.  \u003c\/p\u003e\u003cp\u003e 3. 13. EXCERCISES.  \u003c\/p\u003e\u003cp\u003e 3. 14. ADDITIONAL READING.  \u003c\/p\u003e\u003cp\u003e 3.15. REFERENCES.  \u003c\/p\u003e\u003cp\u003e \u003cb\u003e4. TANDEM MASS SPECTROMETRY.\u003c\/b\u003e  \u003c\/p\u003e\u003cp\u003e 4.1. BASIC PRINCIPLES OF TANDEM MASS SPECTROMETRY.  \u003c\/p\u003e\u003cp\u003e 4.2. TYPES OF SCAN FUNCTIONS.  \u003c\/p\u003e\u003cp\u003e 4.3. ION ACTIVATION AND DISSOCIATION.  \u003c\/p\u003e\u003cp\u003e 4.3.1. Collision-induced Dissociation.  \u003c\/p\u003e\u003cp\u003e 4.3.2. Surface-induced Dissociation.  \u003c\/p\u003e\u003cp\u003e 4.3.3. Absorption of Electromagnetic Radiations.  \u003c\/p\u003e\u003cp\u003e 4.3.4. Electron-capture Dissociation.  \u003c\/p\u003e\u003cp\u003e 4.4. REACTIONS IN TANDEM MASS SPECTROMETRY.  \u003c\/p\u003e\u003cp\u003e 4.5. TANDEM MASS SPECTROMETRY INSTRUMENTATION.  \u003c\/p\u003e\u003cp\u003e 4.5.1. Magnetic Sector Tandem Mass Spectrometers.  \u003c\/p\u003e\u003cp\u003e 4.5.2. Tandem Mass Spectrometry with Multiple Quadrupole Devices.  \u003c\/p\u003e\u003cp\u003e 4.5.3. Tandem Mass Spectrometry with Time-of-Flight Instruments .  \u003c\/p\u003e\u003cp\u003e 4.5.4. Tandem Mass Spectrometry with a Quadrupole Ion Trap Mass Spectrometer.  \u003c\/p\u003e\u003cp\u003e 4.5.5. Tandem Mass Spectrometry with an FT-ICR Mass Spectrometer.  \u003c\/p\u003e\u003cp\u003e 4.5.6. Tandem Mass Spectrometry with Hybrid Instruments.  \u003c\/p\u003e\u003cp\u003e 4.6. OVERVIEW OF THE CHAPTER.  \u003c\/p\u003e\u003cp\u003e 4.7. EXCERCISES.  \u003c\/p\u003e\u003cp\u003e 4.7. ADDITIONAL READING.  \u003c\/p\u003e\u003cp\u003e 4.8. REFERENCES.  \u003c\/p\u003e\u003cp\u003e \u003cb\u003e5. HYPHENATED SEPARATION TECHNIQUES.\u003c\/b\u003e  \u003c\/p\u003e\u003cp\u003e 5.1. BENEFITS OF THE COUPLING OF SEPARATION DEVICES WITH MASS SPECTROMETRY.  \u003c\/p\u003e\u003cp\u003e 5.2. GENERAL CONSIDERATIONS.  \u003c\/p\u003e\u003cp\u003e 5.2.1. Characteristics of an Interface.  \u003c\/p\u003e\u003cp\u003e 5.2.2. Mass Spectral Data Acquisition.  \u003c\/p\u003e\u003cp\u003e 5.2.3. Characteristics of Mass Spectrometers.  \u003c\/p\u003e\u003cp\u003e 5.3. CHROMATOGRAPHIC PROPERTIES.  \u003c\/p\u003e\u003cp\u003e 5.4. GAS CHROMATOGRAPHY\/MASS SPCTROMETRY.  \u003c\/p\u003e\u003cp\u003e 5.4.1 Basic Principles of Gas Chromatography.  \u003c\/p\u003e\u003cp\u003e 5.4.2 Interfaces for the Coupling of Gas Chromatography with Mass Spectrometry.  \u003c\/p\u003e\u003cp\u003e 5.5. LIQUID CHROMATOGRAPHY\/MASS SPECTROMETRY.  \u003c\/p\u003e\u003cp\u003e 5.5.1. Basic Principle of HPLC Separation .  \u003c\/p\u003e\u003cp\u003e 5.5.2 Fast-Flow Liquid Chromatography.  \u003c\/p\u003e\u003cp\u003e 5.6. INTERFACES FOR THE COUPLING OF LIQUID CHROMATO-GRAPHY WITH MASS SPECTROMETRY.  \u003c\/p\u003e\u003cp\u003e 5.6.1. The Moving-belt Interface.  \u003c\/p\u003e\u003cp\u003e 5.6.2. The Direct-Liquid Introduction Probe .  \u003c\/p\u003e\u003cp\u003e 5.6.3. The Continuous-Flow Fast Atom Bombardment Interface.  \u003c\/p\u003e\u003cp\u003e 5.6.4. The Thermospray Interface.  \u003c\/p\u003e\u003cp\u003e 5.6.5. The Particle-beam Interface.  \u003c\/p\u003e\u003cp\u003e 5.6.6. The Electrospray Ionization Interface.  \u003c\/p\u003e\u003cp\u003e 5.6.7. The Atmospheric Pressure Chemical Ionization Interface.  \u003c\/p\u003e\u003cp\u003e 5.6.8. The Atmospheric Pressure Photoionization (APPI) Interface.  \u003c\/p\u003e\u003cp\u003e 5.6.9. The Coupling of LC with TOF-MS.  \u003c\/p\u003e\u003cp\u003e 5.6.10. The Coupling of LC with MALDI-MS.  \u003c\/p\u003e\u003cp\u003e 5.7. MULTI-DIMENSIONAL LC\/MS.  \u003c\/p\u003e\u003cp\u003e 5.8. CAPILLARY ELECTROPHORESIS\/MASS SPECTROMETRY.  \u003c\/p\u003e\u003cp\u003e 5.8.1. The Basic Principles of Capillary Electrophoresis.  \u003c\/p\u003e\u003cp\u003e 5.8.2. Interfaces for the Coupling of Capillary Electrophoresis with Mass Spectrometry.  \u003c\/p\u003e\u003cp\u003e 5.9. AFFINITY CHROMATOGRAPHY\/MASS SPECTROMETRY .  \u003c\/p\u003e\u003cp\u003e 5.10. SUPERCRITICAL-FLUID CHROMATOGRAPHY\/MASS SPECTROMETRY.  \u003c\/p\u003e\u003cp\u003e 5.11. THE COUPLING OF PLANAR CHROMATOGRAPHY WITH MASS SPECTROMETRY.  \u003c\/p\u003e\u003cp\u003e 5.12. OVERVIEW OF THE CHAPTER.  \u003c\/p\u003e\u003cp\u003e 5.13. EXCERCISES.  \u003c\/p\u003e\u003cp\u003e 5.14. ADDITIONAL READING.  \u003c\/p\u003e\u003cp\u003e 5.15. REFERENCES.  \u003c\/p\u003e\u003cp\u003e \u003cb\u003ePART 2: ORGANIC AND INORGAANIC MASS SPECTROMETRY .\u003c\/b\u003e  \u003c\/p\u003e\u003cp\u003e \u003cb\u003e6. ORGANIC MASS SPECTROMETRY.\u003c\/b\u003e  \u003c\/p\u003e\u003cp\u003e 6.1. DETERMINATION OF MOLECULAR MASS.  \u003c\/p\u003e\u003cp\u003e 6.1.1. Molecular Mass Measurements at Low-mass Resolving Power.  \u003c\/p\u003e\u003cp\u003e 6.1.2. Molecular Mass Measurements at High-mass Resolving Power .  \u003c\/p\u003e\u003cp\u003e 6.1.3. Molecular Mass Measurements by ESI and MALDI.  \u003c\/p\u003e\u003cp\u003e 6.1.4. Mass Calibration Standards.  \u003c\/p\u003e\u003cp\u003e 6.2. MOLECULAR FORMULA FROM ACCURATE MASS VALUES .  \u003c\/p\u003e\u003cp\u003e 6.3. MOLECULAR FORMULA FROM ISOTOPIC PEAKS.  \u003c\/p\u003e\u003cp\u003e 6.4. GENERAL GUIDELINES FOR THE INTERPRETATION OF A MASS SPECTRUM .  \u003c\/p\u003e\u003cp\u003e 6.4.1. Odd- and Even-electron Ions.  \u003c\/p\u003e\u003cp\u003e 6.4.2. Recognize the Molecular Ion.  \u003c\/p\u003e\u003cp\u003e 6.4.3. The Nitrogen Rule .  \u003c\/p\u003e\u003cp\u003e 6.4.4. The Rings Plus Double Bonds (R + DB) Value .  \u003c\/p\u003e\u003cp\u003e 6.4.5. Systematic Steps to Interpret a Mass Spectrum.  \u003c\/p\u003e\u003cp\u003e 6.4.6. Mass Spectral Compilations.  \u003c\/p\u003e\u003cp\u003e 6.5. FRAGMENTATION PROCESSES.  \u003c\/p\u003e\u003cp\u003e 6.5.1. Simple Bond-cleavage Reactions.  \u003c\/p\u003e\u003cp\u003e 6.5.2. Rearrangement Reactions.  \u003c\/p\u003e\u003cp\u003e 6.5.3. Fragmentation of Cyclic Structures.  \u003c\/p\u003e\u003cp\u003e 6.5.4. Differentiation of Isomeric Structures.  \u003c\/p\u003e\u003cp\u003e 6.5.5. Structurally Diagnostic Fragment Ions.  \u003c\/p\u003e\u003cp\u003e 6.6. FRAGMENTATION REACTIONS OF SPECIFIC CLASSES OF COMPOUNDS.  \u003c\/p\u003e\u003cp\u003e 6.6.1. Hydrocarbons.  \u003c\/p\u003e\u003cp\u003e 6.6. FRAGMENTATION REACTIONS OF SPECIFIC CLASSES OF COMPOUNDS.  \u003c\/p\u003e\u003cp\u003e 6.6.1. Hydrocarbons.  \u003c\/p\u003e\u003cp\u003e 6.6. FRAGMENTATION REACTIONS OF SPECIFIC CLASSES OF COMPOUNDS.  \u003c\/p\u003e\u003cp\u003e 6.6.1. Hydrocarbons.  \u003c\/p\u003e\u003cp\u003e 6.6.2. Alcohols.  \u003c\/p\u003e\u003cp\u003e 6.6.3. Ethers.  \u003c\/p\u003e\u003cp\u003e 6.6.4. Aldehydes and Ketones.  \u003c\/p\u003e\u003cp\u003e 6.6.5. Carboxylic Acids.  \u003c\/p\u003e\u003cp\u003e 6.6.6. Esters.  \u003c\/p\u003e\u003cp\u003e 6.6.7. Nitrogen-containing Compounds6.6.8. Sulfur-containing Compounds.  \u003c\/p\u003e\u003cp\u003e 6.6.8. Sulfur-containing Compounds.  \u003c\/p\u003e\u003cp\u003e 6.6.9. Halogen-containing Compounds.  \u003c\/p\u003e\u003cp\u003e 6.7. THEORY OF ION DISSOCIATION.  \u003c\/p\u003e\u003cp\u003e 6.8. STRUCTURE DETERMINATION OF GAS-PHASE ORGANIC IONS.  \u003c\/p\u003e\u003cp\u003e 6.9. OVERVIEW OF THE CHAPTER.  \u003c\/p\u003e\u003cp\u003e 6. 10. EXCERCISES.  \u003c\/p\u003e\u003cp\u003e 6.11. ADDITIONAL READING.  \u003c\/p\u003e\u003cp\u003e 6.12. REFERENCES.  \u003c\/p\u003e\u003cp\u003e \u003cb\u003e7. INORGANIC MASS SPECTROMETRY.\u003c\/b\u003e  \u003c\/p\u003e\u003cp\u003e 7.1. IONIZTION OF INORGANIC COMPOUNDS.  \u003c\/p\u003e\u003cp\u003e 7.2. THERMAL IONIZATION MASS SPECTROMETRY.  \u003c\/p\u003e\u003cp\u003e 7.3. SPARK-SOURCE MASS SPECTROMETRY (SSMS).  \u003c\/p\u003e\u003cp\u003e 7.4. GLOW DISCHARGE IONIZATION MASS SPECTROMETRY.  \u003c\/p\u003e\u003cp\u003e 7.5. INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY.  \u003c\/p\u003e\u003cp\u003e 7.5.1. Inductively Coupled Plasma Ion Source .  \u003c\/p\u003e\u003cp\u003e 7.5.2. Coupling of the ICP Source with Mass Spectrometry .  \u003c\/p\u003e\u003cp\u003e 7.5.3. Sample Introduction Systems for the ICP Source.  \u003c\/p\u003e\u003cp\u003e 7.5.4. Spectral Interferences.  \u003c\/p\u003e\u003cp\u003e 7.5.5. Laser Ablation-ICPMS.  \u003c\/p\u003e\u003cp\u003e 7.6. RESONANCE IONIZATION MASS SPCTROMETRY.  \u003c\/p\u003e\u003cp\u003e 7.7. ISOTOPE RATIO MASS SPCTROMETRY.  \u003c\/p\u003e\u003cp\u003e 7.7.1. Isotope Ratio MS Systems .  \u003c\/p\u003e\u003cp\u003e 7.8. ACCELERATOR MASS SPECTROMETRY.  \u003c\/p\u003e\u003cp\u003e 7.9. ISOTOPE DILUTION MASS SPECTROMETRY.  \u003c\/p\u003e\u003cp\u003e 7.10. OVERVIEW OF THE CHAPTER.  \u003c\/p\u003e\u003cp\u003e 7.11. EXCERCISES.  \u003c\/p\u003e\u003cp\u003e 7.12. ADDITIONAL READING.  \u003c\/p\u003e\u003cp\u003e 7.13. REFERENCES.  \u003c\/p\u003e\u003cp\u003e \u003cb\u003ePART 3: BIOLOGICAL MASS SPECTROMETRY.\u003c\/b\u003e  \u003c\/p\u003e\u003cp\u003e \u003cb\u003e8. PROTEINS AND PEPTIDES: STRUCTURE DETERMINATION.\u003c\/b\u003e  \u003c\/p\u003e\u003cp\u003e 8.1. INTRODUCTION .  \u003c\/p\u003e\u003cp\u003e 8.1.1. Structure of Proteins .  \u003c\/p\u003e\u003cp\u003e 8.2. DETERMINATION OF THE SEQUENCE OF A PROTEIN.  \u003c\/p\u003e\u003cp\u003e 8.3. GENERAL PROTOCOL FOR THE AMINO ACID SEQUENCE DETERMINATION OF PROTEINS .  \u003c\/p\u003e\u003cp\u003e 8.3.1. Homogenization and Subcellular Fractionation.  \u003c\/p\u003e\u003cp\u003e 8.3.2. Enrichment and Purification of Proteins.  \u003c\/p\u003e\u003cp\u003e 8.4. MOLECULAR MASS MEASUREMENT OF PROTEINS.  \u003c\/p\u003e\u003cp\u003e 8.5. PEPTIDE MASS MAPPING.  \u003c\/p\u003e\u003cp\u003e 8.5.1. Reduction and Carboxymethylation.  \u003c\/p\u003e\u003cp\u003e 8.5.2. Cleavage of Proteins.  \u003c\/p\u003e\u003cp\u003e 8.5.3. Mass Spectrometry Analysis of Peptide Maps .  \u003c\/p\u003e\u003cp\u003e 8.6. PROTEOMICS.  \u003c\/p\u003e\u003cp\u003e 8.6.1. Strategies for Proteomics.  \u003c\/p\u003e\u003cp\u003e 8.7. QUANTITATIVE PROTEOMICS.  \u003c\/p\u003e\u003cp\u003e 8.8. BIOMARKER DISCOVERY.  \u003c\/p\u003e\u003cp\u003e 8.9. DE NOVO PROTEIN SEQUENCING .  \u003c\/p\u003e\u003cp\u003e 8.9. DETERMINATION OF THE AMINO ACID SEQUENCE OF PEPTIDES.  \u003c\/p\u003e\u003cp\u003e 8.9.1. Peptide Fragmentation Rules .  \u003c\/p\u003e\u003cp\u003e 8.9.2. Mass Spectrometry Techniques for Sequence Determination of Peptides.  \u003c\/p\u003e\u003cp\u003e 8.9.3. Guidelines to Obtain the Amino Acid Sequence from a Mass Spectrum.  \u003c\/p\u003e\u003cp\u003e 8.10. OVERVIEW OF THE CHAPTER.  \u003c\/p\u003e\u003cp\u003e 8.11. EXCERCISES.  \u003c\/p\u003e\u003cp\u003e 8.12. ADDITIONAL READING.  \u003c\/p\u003e\u003cp\u003e 8.13. REFERENCES.  \u003c\/p\u003e\u003cp\u003e \u003cb\u003e9. Proteins and Peptides: Post-Translational Modifications Disulfide Bonds in Proteins.\u003c\/b\u003e  \u003c\/p\u003e\u003cp\u003e 9.1. TRADITIONAL APPROACHES TO IDENTIFY DISULFIDE BONDS .  \u003c\/p\u003e\u003cp\u003e 9.2. MASS SPECTROMETRY-BASED METHODS TO IDENTIFY DISULFIDE BONDS.  \u003c\/p\u003e\u003cp\u003e 9.2.1. Determination of the Number of Disulfide Bonds .  \u003c\/p\u003e\u003cp\u003e 9.2.2. Generation of the Disulfide-containing Peptides .  \u003c\/p\u003e\u003cp\u003e 9.2.3. Identification of Disulfide-containing Peptides by FAB-MS .  \u003c\/p\u003e\u003cp\u003e 9.2.4. Identification of the Disulfide-containing Peptides by MALDI-MS .  \u003c\/p\u003e\u003cp\u003e 9.2.5. Identification of the Disulfide-containing Peptides by Electron-capture Dissociation (ECD) .  \u003c\/p\u003e\u003cp\u003e 9.2.6. Identification of Disulfide-containing Peptides by Tandem MS .  \u003c\/p\u003e\u003cp\u003e \u003cb\u003eANALYSIS OF PHOPHOPROTEINS AND PHOSPHO-PROTEOMICS.\u003c\/b\u003e  \u003c\/p\u003e\u003cp\u003e 9.3. 32[P]-Labeling for the Analysis of Phosphoproteins.  \u003c\/p\u003e\u003cp\u003e 9.4. MASS SPECTROMETRY PROTOCOL FOR THE ANALYSIS OF PHOSPHOPROTEINS.  \u003c\/p\u003e\u003cp\u003e 9.4.1. Cleavage of Purified Phosphoproteins .  \u003c\/p\u003e\u003cp\u003e 9.4.2. Fractionation of Peptide Fragments in the Digest .  \u003c\/p\u003e\u003cp\u003e 9.4.3. Determination of the Average Number of Phosphate Groups .  \u003c\/p\u003e\u003cp\u003e 9.4.4. Identification of Phosphopeptides .  \u003c\/p\u003e\u003cp\u003e 9.4.5. Identification of Phosphorylation Sites .  \u003c\/p\u003e\u003cp\u003e \u003cb\u003eANALYSIS OF GLYCOPROTEINS.\u003c\/b\u003e  \u003c\/p\u003e\u003cp\u003e 9.5. STRUCTURAL DIVERSITY OF GLYCOPROTEINS.  \u003c\/p\u003e\u003cp\u003e 9.6. ANALYSIS OF GLYCOPROTEINS.  \u003c\/p\u003e\u003cp\u003e 9.6.1. Molecular Mass Determination of Glycoproteins.  \u003c\/p\u003e\u003cp\u003e 9.6.2. Identification of Glycosylation.  \u003c\/p\u003e\u003cp\u003e 9.6.3. Site of Glycosylation .  \u003c\/p\u003e\u003cp\u003e 9.7. OVERVIEW OF THE CHAPTER.  \u003c\/p\u003e\u003cp\u003e 9.8. EXCERCISES.  \u003c\/p\u003e\u003cp\u003e 9.9. REFERENCES.  \u003c\/p\u003e\u003cp\u003e \u003cb\u003e10. Proteins and Peptides: Higher-Order Structures.\u003c\/b\u003e  \u003c\/p\u003e\u003cp\u003e 10.1. CHARGE-STATE DISTRIBUTION.  \u003c\/p\u003e\u003cp\u003e 10.2. HYDROGEN\/DEUTERIUM EXCHANGE TO STUDY CONFORMATIONAL STATES OF PROTEINS.  \u003c\/p\u003e\u003cp\u003e 10.2.1. Folding\/Unfolding Dynamics of Proteins.  \u003c\/p\u003e\u003cp\u003e 10.2.2. Experimental Measurements of the Amide Hydrogen Isotopic Exchange .  \u003c\/p\u003e\u003cp\u003e 10.3. CHEMICAL CROSS-LINKING AS A PROBE FOR THE 3-D STRUCTURE OF PROTEINS.  \u003c\/p\u003e\u003cp\u003e 10.4. ION MOBILITY MEASUREMENTS TO STUDY PROTEIN CONFORMATIONAL CHANGES.  \u003c\/p\u003e\u003cp\u003e 10.5. OVERVIEW OF THE CHAPTER.  \u003c\/p\u003e\u003cp\u003e 10.6. EXCERCISES.  \u003c\/p\u003e\u003cp\u003e 10.7. ADDITIONAL READING.  \u003c\/p\u003e\u003cp\u003e 10.8. REFERENCES.  \u003c\/p\u003e\u003cp\u003e \u003cb\u003e11. Characterization of Oligosaccharides.\u003c\/b\u003e  \u003c\/p\u003e\u003cp\u003e 11.1. STRUCTURAL DIVERSITY IN OLIGOSACCHARIDES.  \u003c\/p\u003e\u003cp\u003e 11.2. CLASSES OF GLYCANS.  \u003c\/p\u003e\u003cp\u003e 11.3. MASS SPECTROMETRIC METHODS FOR COMPLETE STRCUTURE ELUCIDATION OF OLIGOSACCHARIDES.  \u003c\/p\u003e\u003cp\u003e 11.3.1. Release of Glycans.  \u003c\/p\u003e\u003cp\u003e 11.3.2. Derivatization of Carbohydrate chains.  \u003c\/p\u003e\u003cp\u003e 11.3.3. Composition Analysis by GC\/MS.  \u003c\/p\u003e\u003cp\u003e 11.3.4. Linkage Analysis by GC\/MS.  \u003c\/p\u003e\u003cp\u003e 11.3.5. Rapid Identification by a Precursor-ion Scan .  \u003c\/p\u003e\u003cp\u003e 11.3.6. Composition Analysis by Direct Mass Measurement.  \u003c\/p\u003e\u003cp\u003e 11.3.7. Structure Determination of Oligosaccharides by Sequential Digestion.  \u003c\/p\u003e\u003cp\u003e 11.3.8. Tandem Mass Spectrometry for Structural Analysis of Carbohydrates.  \u003c\/p\u003e\u003cp\u003e 11.4. OVERVIEW OF THE CHAPTER.  \u003c\/p\u003e\u003cp\u003e 11.5. EXCERCISES.  \u003c\/p\u003e\u003cp\u003e 11.6. REFERENCES.  \u003c\/p\u003e\u003cp\u003e \u003cb\u003e12. Characterization of Lipids.\u003c\/b\u003e  \u003c\/p\u003e\u003cp\u003e 12.1. CLASSIFICATION AND STRUCTURES OF LIPIDS.  \u003c\/p\u003e\u003cp\u003e 12.2. MASS SPECTROMETRY OF FATTY ACIDS AND ACYLGLYCEROLS.  \u003c\/p\u003e\u003cp\u003e 12.2.1. Analysis of Fatty Acids.  \u003c\/p\u003e\u003cp\u003e 12.2.2. Analysis of Acylglycerols.  \u003c\/p\u003e\u003cp\u003e 12.3. MASS SPECTROMETRY OF PHOSPHOLIPIDS.  \u003c\/p\u003e\u003cp\u003e 12.4. MASS SPECTROMETRY OF GLYCOLIPIDS.  \u003c\/p\u003e\u003cp\u003e 12.5. ANALYSIS OF BILE ACIDS AND STEROIDS.  \u003c\/p\u003e\u003cp\u003e 12.6. ANALYSIS OF EICOSANOIDS.  \u003c\/p\u003e\u003cp\u003e 12.7. LIPIDOMICS.  \u003c\/p\u003e\u003cp\u003e 12.8. OVERVIEW OF THE CHAPTER.  \u003c\/p\u003e\u003cp\u003e 12.9. EXCERCISES .  \u003c\/p\u003e\u003cp\u003e 12.10. REFERENCES.  \u003c\/p\u003e\u003cp\u003e \u003cb\u003e13. Structure Determination of Oligonucleotides.\u003c\/b\u003e  \u003c\/p\u003e\u003cp\u003e 13.1. STRUCTURES OF NUCLEOTIDES AND OLIGONUCLEOTIDES.  \u003c\/p\u003e\u003cp\u003e 13.2. MASS SPECTROMETRY ANALYSIS OF NUCLEOSIDES AND NUCLEOTIDES.  \u003c\/p\u003e\u003cp\u003e 13.3. CLEAVAGE OF OLIGONUCLEOTIDES.  \u003c\/p\u003e\u003cp\u003e 13.4. MOLECULAR MASS DETERMINATION OF OLIGONUCLEOTIDES.  \u003c\/p\u003e\u003cp\u003e 13.4.1. Electrospray Ionization for the Molecular Mass Determination .  \u003c\/p\u003e\u003cp\u003e 13.4.2. Matrix-assisted Laser Desorption\/Ionization for Molecular Mass Determination.  \u003c\/p\u003e\u003cp\u003e 13.4.3. Base Composition from an Accurate Mass Measurement.  \u003c\/p\u003e\u003cp\u003e 13.5. MASS SPECTROMETRY SEQUENCING OF OLIGONUCLEAOTIDES.  \u003c\/p\u003e\u003cp\u003e 13.5.1. Gas-phase Fragmentation for Oligonucleotide Sequencing.  \u003c\/p\u003e\u003cp\u003e 13.5.2. Solution-phase Techniques for Oligonucleotide Sequencing .  \u003c\/p\u003e\u003cp\u003e 13.6. OVERVIEW OF THE CHAPTER.  \u003c\/p\u003e\u003cp\u003e 13.7. EXCERCISES.  \u003c\/p\u003e\u003cp\u003e 13.8. REFERENCES.  \u003c\/p\u003e\u003cp\u003e \u003cb\u003e14. Quantitative Analysis.\u003c\/b\u003e  \u003c\/p\u003e\u003cp\u003e 14.1. ADVANTAGES OF MASS SPECTROMETRY.  \u003c\/p\u003e\u003cp\u003e 14.2. DATA ACQUISITION.  \u003c\/p\u003e\u003cp\u003e 14.2.1. Selected-ion Monitoring.  \u003c\/p\u003e\u003cp\u003e 14.2.2. Selected-reaction Monitoring.  \u003c\/p\u003e\u003cp\u003e 14.3. CALIBRATION.  \u003c\/p\u003e\u003cp\u003e 14.3.1. External Standard Method.  \u003c\/p\u003e\u003cp\u003e 14.3.2. Standard Addition Method.  \u003c\/p\u003e\u003cp\u003e 14.3.3. Internal Standard Method .  \u003c\/p\u003e\u003cp\u003e 14.4 VALIDATION OF A QUANTITATIVE METHOD.  \u003c\/p\u003e\u003cp\u003e 14.5. SELECTED EXAMPLES.  \u003c\/p\u003e\u003cp\u003e 14.5.1. Applications of Gas Chromatography\/Mass Spectrometry.  \u003c\/p\u003e\u003cp\u003e 14.5.2. Applications of Liquid Chromatography\/Mass Spectrometry .  \u003c\/p\u003e\u003cp\u003e 14.5.3. Applications of MALDI-MS.  \u003c\/p\u003e\u003cp\u003e 14.6. OVERVIW OF THE CHAPTER.  \u003c\/p\u003e\u003cp\u003e 14.7. EXCERCISES.  \u003c\/p\u003e\u003cp\u003e 14.8. ADDITIONAL READING.  \u003c\/p\u003e\u003cp\u003e 14.9. REFERENCES.  \u003c\/p\u003e\u003cp\u003e \u003cb\u003e15. Misclaneous Topics.\u003c\/b\u003e  \u003c\/p\u003e\u003cp\u003e 15.1. ENZYME KINETICS.  \u003c\/p\u003e\u003cp\u003e 15.1.1. Theory.  \u003c\/p\u003e\u003cp\u003e 15.1.2. Reaction Monitoring.  \u003c\/p\u003e\u003cp\u003e 15.2. IMAGING MASS SPECTROMETRY.  \u003c\/p\u003e\u003cp\u003e 15.2.2. Imaging with SIMS.  \u003c\/p\u003e\u003cp\u003e 15.2.2. Imaging with MALDI-MS.  \u003c\/p\u003e\u003cp\u003e 15.3. ANALYSIS OF MICROORGANISMS.  \u003c\/p\u003e\u003cp\u003e 15.3.1. Bacterial Identification.  \u003c\/p\u003e\u003cp\u003e 15.3.2. Analysis of Viruses.  \u003c\/p\u003e\u003cp\u003e 15.4. CLINICAL MASS SPECTROMETRY.  \u003c\/p\u003e\u003cp\u003e 15.4.1. Low Molecular Mass Compounds as Biomarkers of Disease.  \u003c\/p\u003e\u003cp\u003e 15.4.2. Analysis of DNA to diagnose Genetic Disorders.  \u003c\/p\u003e\u003cp\u003e 15.4.3. Proteins as Biomarkers of Disease .  \u003c\/p\u003e\u003cp\u003e 15.5. METABOLOMICS.  \u003c\/p\u003e\u003cp\u003e 15.6. FORENSIC MASS SPECTROMETRY.  \u003c\/p\u003e\u003cp\u003e 15.6.1. Analysis of Banned Substances of Abuse.  \u003c\/p\u003e\u003cp\u003e 15.6.2 Analysis of Explosives.  \u003c\/p\u003e\u003cp\u003e 15.6.3. Analysis of Glass and Paints.  \u003c\/p\u003e\u003cp\u003e 15.6.4. Authenticity of Questioned Documents.  \u003c\/p\u003e\u003cp\u003e 15.6.5. Mass spectrometry in Bioterror Defense.  \u003c\/p\u003e\u003cp\u003e 15.7. SCREENING COMBINATORIAL LIBRARIES.  \u003c\/p\u003e\u003cp\u003e 15.7.1. Combinatorial Synthetic Procedures.  \u003c\/p\u003e\u003cp\u003e 15.7.2. Screening Methods.  \u003c\/p\u003e\u003cp\u003e 15.8. ADDITIONAL READING.  \u003c\/p\u003e\u003cp\u003e 15.9. REFERENCES .  \u003c\/p\u003e\u003cp\u003e Appendix A: Abbreviations.  \u003c\/p\u003e\u003cp\u003e Appendix B: Physical Constants, Units, and Conversion Factors .  \u003c\/p\u003e\u003cp\u003e Appendix C: Isotopes of Naturally Occurring Elements and their Abundances.  \u003c\/p\u003e\u003cp\u003e Appendix D: Reference Ions and Their Exact Masses.  \u003c\/p\u003e\u003cp\u003e Appendix E: Internet Resources.  \u003c\/p\u003e\u003cp\u003e Appendix F. Answers and Hints to Exercises.  \u003c\/p\u003e\u003cp\u003e Index. \u003c\/p\u003e","brand":"John Wiley \u0026 Sons Inc","offers":[{"title":"Default Title","offer_id":49402652426583,"sku":"9780471682295","price":125.96,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9780471682295.jpg?v=1730481127","url":"https:\/\/bookcurl.com\/products\/fundamentals-of-contemporary-mass-spectrometry-16-wiley-series-on-mass-spectrometry-9780471682295","provider":"Book Curl","version":"1.0","type":"link"}