{"product_id":"drosophila-9781071625439","title":"Drosophila","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003eThis third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and cell lineage tracing, protein inhibition and tagging, optogenetic and optochemical control of tissue mechanics, AFM measurements, sample preparation for electron microscopy, live imaging of different tissues and organs, and quantitative image analysis. Written in the format of the highly successful Methods in Molecular Biology series, chapters include an introduction to their topic, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfallsAuthoritative and cutting-edge,Drosophila: M\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cp\u003e                        \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e1.              \u003c\/b\u003eUsing FlyBase, a database of \u003ci\u003eDrosophila\u003c\/i\u003e genes and genetics \u003c\/p\u003e  \u003cp\u003eVictoria K. Jenkins, Aoife Larkin, Jim Thurmond and The FlyBase Consortium \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e2.              \u003c\/b\u003eThe Q-System: A versatile repressible binary expression system\u003c\/p\u003e  \u003cp\u003eOrsolya Fölsz, Chun-Chieh Lin, Darya Task, Olena Riabinina and Christopher J. Potter\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e3.              \u003c\/b\u003eResources and methods for the analysis of microRNA function in \u003ci\u003eDrosophila\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003eSromana Mukherjee and Nicholas Sokol\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e4.              \u003c\/b\u003eAnalysis of single-cell transcriptome data in \u003ci\u003eDrosophila\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003eSchayan Yousefian, Maria Jelena Musillo and Josephine Bageritz                                \u003csup\u003e\u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e5.              \u003c\/b\u003ePrime editing for precise genome engineering in \u003ci\u003eDrosophila\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003eJustin A. Bosch and Norbert Perrimon\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e6.              \u003c\/b\u003eCRISPR\/Cas9 mediated precise and efficient genome editing in \u003ci\u003eDrosophila\u003c\/i\u003e \u003c\/p\u003e  \u003cp\u003eKevin G. Nyberg and Richard W. Carthew \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e7.              \u003c\/b\u003eTissue-specific CRISPR-Cas9 screening in \u003ci\u003eDrosophila\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003eFillip Port and Michael Boutros\u003c\/p\u003e  \u003cp\u003e8.              CRISPR-based transcriptional activation in \u003ci\u003eDrosophila\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003eYuting Han, Xinyi Lu, Yutong Li, Yuhao Qiu, Xizhi Dong, Xiaochen Li, Xu Si, Qingfei Liu, Jian-Quan Ni\u003c\/p\u003e  \u003cb\u003e9.              \u003c\/b\u003eTracing and manipulating \u003ci\u003eDrosophila\u003c\/i\u003e cell lineages based on CRISPR: CaSSA and CLADES\u003cp\u003e\u003c\/p\u003e  \u003cp\u003eJorge Garcia-Marques and Tzumin Lee\u003c\/p\u003e  \u003cp\u003e10.           Studying protein function using nanobodies and other protein binders in \u003ci\u003eDrosophila\u003c\/i\u003e \u003c\/p\u003e  \u003cp\u003eKatarzyna Lepeta, Milena Bauer, Gustavo Aguilar, Alessandra Vigano, Shinya Matsuda and Markus Affolter \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e11.           \u003c\/b\u003eAnchor Away: A system for fast inhibition of proteins in \u003ci\u003eDrosophila\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003ePablo Sanchez Bosch\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e12.           \u003c\/b\u003eTagging \u003ci\u003eDrosophila\u003c\/i\u003e proteins with genetically-encoded fluorophores\u003c\/p\u003e  \u003cp\u003eJerome Avellaneda and Frank Schnorrer \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e13.           \u003c\/b\u003eOptogenetic methods to control tissue mechanics in \u003ci\u003eDrosophila\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003eDaniel Krueger and Stefano De Renzis\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e14.           \u003c\/b\u003eOptochemical control of cell contractility in \u003ci\u003eDrosophila\u003c\/i\u003e embryos \u003c\/p\u003e  Deqing Kong and Jörg Großhans\u003cp\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e15.           \u003c\/b\u003eStiffness measurement of \u003ci\u003eDrosophila\u003c\/i\u003e egg chambers by atomic force microscopy \u003c\/p\u003e  Uwe Töpfer, Karla Yanín Guerra Santillán and Elisabeth Fischer-Friedrich\u003cp\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e16.           \u003c\/b\u003eCultivation and live imaging of \u003ci\u003eDrosophila\u003c\/i\u003e imaginal discs\u003c\/p\u003e  \u003cp\u003eNatalie A. Dye \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e17.           \u003c\/b\u003eSample preparation and imaging of the pupal \u003ci\u003eDrosophila\u003c\/i\u003e abdominal epidermis  \u003c\/p\u003e  \u003cp\u003eDaiki Umetsu\u003c\/p\u003e  \u003cp\u003e18.           FlyClear: A tissue clearing technique for high-resolution microscopy of \u003ci\u003eDrosophila\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003eMarko Pende, Saiedeh Saghafi, Klaus Becker, Thomas Hummel and Hans-Ulrich Dodt\u003c\/p\u003e  \u003cp\u003e19.           Preparation of \u003ci\u003eDrosophila\u003c\/i\u003e tissues and organs for transmission electron microscopy\u003c\/p\u003e  \u003cp\u003eOlympia-Ekaterini Psathaki and Achim Paululat\u003c\/p\u003e  \u003cp\u003e20.           Segmentation and quantitative analysis of epithelial tissues \u003c\/p\u003e  \u003cp\u003eBenoit Aigouy and Benjamin Prud’homme\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e21.           \u003c\/b\u003eGenetically-encoded sensors to study metabolism in \u003ci\u003eDrosophila\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003eEllen McMullen, Helen Hertenstein, Stephan Müller and Stefanie Schirmeier\u003c\/p\u003e","brand":"Springer-Verlag New York Inc.","offers":[{"title":"Default Title","offer_id":48738193146199,"sku":"9781071625439","price":104.49,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9781071625439.jpg?v=1723811807","url":"https:\/\/bookcurl.com\/products\/drosophila-9781071625439","provider":"Book Curl","version":"1.0","type":"link"}