{"product_id":"directed-evolution-9781071621516","title":"Directed Evolution","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003eThis volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cp\u003ePreface…\u003cbr\u003eTable of Contents…\u003cbr\u003eContributing Authors…\u003cbr\u003e\u003cbr\u003e1. Designing Overlap Extension PCR Primers for Protein Mutagenesis: A Programmatic Approach\u003cbr\u003eXiaofang Huang, Liangting Xu, Chuyun Bi, Zhao Lili, Zhang Limei, Xuanyang Chen, Shiqian Qi, and Shiqiang Lin\u003cbr\u003e\u003cbr\u003e2. Recombination of Single Beneficial Substitutions Obtained from Protein Engineering by Computer-Assisted Recombination (CompassR)\u003cbr\u003eHaiyang Cui, Mehdi D. Davari, and Ulrich Schwaneberg\u003cbr\u003e\u003cbr\u003e3. Non-Degenerate Saturation Mutagenesis: Library Construction and Analysis via MAX and ProxiMAX Randomization\u003cbr\u003eAnupama Chembath, Ben P.G. Wagstaffe, Mohammed Ashraf, Marta M. Ferreira Amaral, Laura Frigotto, and Anna V. Hine\u003cbr\u003e\u003cbr\u003e4. Antha-Guided Automation of Darwin Assembly for the Construction of Bespoke Gene Libraries\u003cbr\u003eP. Handal-Marquez, M. Koch, D. Kestemont, S. Arangundy-Franklin, and V.B. Pinheiro\u003cbr\u003e\u003cbr\u003e5. SpeedyGenesXL An Automated, High-Throughput Platform for the Preparation of Bespoke Ultra-Large Variant Libraries for Directed Evolution\u003cbr\u003eJoanna C. Sadler, Neil Swainston, Mark S. Dunstan, Andrew Currin, and Douglas B. Kell\u003cbr\u003e\u003cbr\u003e6. Facile Assembly of Combinatorial Mutagenesis Libraries using Nicking Mutagenesis\u003cbr\u003eMonica B. Kirby and Timothy A. Whitehead\u003cbr\u003e\u003cbr\u003e7. GeneORator: An Efficient Method for the Systematic Mutagenesis of Entire Genes\u003cbr\u003eLucy Green, Nigel S. Scrutton, and Andrew Currin\u003cbr\u003e\u003cbr\u003e8. Rapid Cloning of Random Mutagenesis Libraries using PTO-QuickStep\u003cbr\u003ePawel Jajesniak, Kang Lan Tee, and Tuck Seng Wong\u003cbr\u003e\u003cbr\u003e9. Construction of Strong Promoters by Assembling Sigma Factor Binding Motifs\u003cbr\u003eYonglin Zhang, Yang Wang, Jianghua Li, Chao Wang, Guocheng Du, and Zhen Kang\u003cbr\u003e\u003cbr\u003e10. Application of Restriction Free (RF) Cloning in Circular Permutation\u003cbr\u003eBoudhayan Bandyopadhyay and Yoav Peleg\u003cbr\u003e\u003cbr\u003e11. Site-Directed Mutagenesis Method Mediated by Cas9\u003cbr\u003eWanping Chen, Wenwen She, Aitao Li, Chao Zhai, and Lixin Ma\u003cbr\u003e\u003cbr\u003e12. Directed Evolution of Transcription Factor-Based Biosensors for Altered Effector Specificity\u003cbr\u003eLeopoldo Ferreira Marques Machado and Neil Dixon\u003cbr\u003e\u003cbr\u003e13. A Screening Method for P450 BM3 Mutant Libraries using Multiplexed Capillary Electrophoresis for Detection of Enzymatically Converted Compounds\u003cbr\u003eAnna Gärtner, Gustavo de Almeida Santos, Anna Joëlle Ruff, and Ulrich Schwaneberg\u003cbr\u003e\u003cbr\u003e14. Directed Evolution of Glycosyltransferases by a Single-Cell Ultrahigh-Throughput FACS-Based Screening Method\u003cbr\u003eYumeng Tan, Xue Zhang, Yan Feng, and Guangyu Yang\u003cbr\u003e\u003cbr\u003e15. Learning Strategies in Protein Directed Evolution\u003cbr\u003eXavier F. Cadet, Jean Christophe Gelly, Aster van Noord, Frédéric Cadet, and Carlos G. Acevedo-Rocha\u003cbr\u003e\u003cbr\u003eSubject Index List…\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e","brand":"Springer-Verlag New York Inc.","offers":[{"title":"Default Title","offer_id":49406772052311,"sku":"9781071621516","price":170.99,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9781071621516.jpg?v=1730497055","url":"https:\/\/bookcurl.com\/products\/directed-evolution-9781071621516","provider":"Book Curl","version":"1.0","type":"link"}