{"product_id":"cryopreservation-and-freezedrying-protocols-9781071607824","title":"Cryopreservation and FreezeDrying Protocols","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003eThis fourth edition explores fully up-to-date standardly used cryopreservation, vitrification, and freeze-drying protocols for specimens that are used for research purposes, conservation of genetic reserves, and applications in agriculture and medicine. Beginning with a section on the fundamentals as well as the use of mathematical modeling to solve cryobiological problems, the book continues with sections on technological aspects of freezing and drying, analytical methods to study protectant loading of cells and tissues, cell behavior during freezing and drying, and thermodynamic properties of preservation solutions, as well as cryopreservation, vitrification, and freeze-drying protocols for a wide variety of samples and different applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips o\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003ePart I: Fundamental Aspects of Cryopreservation and Freeze-Drying\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e1. Principles Underlying Cryopreservation and Freeze-Drying of Cells and Tissues\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Willem F. Wolkers and Harriëtte Oldenhof\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e2. Principles of Ice-Free Cryopreservation by Vitrification\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Gregory M. Fahy and Brian Wowk\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e3. The Principles of Freeze-Drying\u003csup\u003e \u003c\/sup\u003eand Application of Analytical Technologies\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Kevin R. Ward and Paul Matejtschuk\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e4. Mathematical Modeling and Optimization of Cryopreservation in Single Cells\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            James D. Benson\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e5. Mathematical Modeling of Protectant Transport in Tissues\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Ross M. Warner and Adam Z. Higgins\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003ePart II: Technologies and Methods to Study Freezing and Drying\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e6. Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Peter Kilbride and Julie Meneghel\u003c\/p\u003e   \u003cp\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e7. Microwave- and Laser-Assisted Drying for the Anhydrous Preservation of Biologics\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Shangping Wang, Susan Trammell, and Gloria D. Elliott\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e8. High-Speed Video Cryomicroscopy for Measurement of Intracellular Ice Formation Kinetics\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Jens O.M. Karlsson\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e9. Use of Ice Recrystallization Inhibition Assays to Screen for Compounds that Inhibit Ice Recrystallization\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Anna A. Ampaw, August Sibthorpe, and Robert N. Ben\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e10. DSC Analysis of Thermophysical Properties for Biomaterials and Formulations\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Wendell Q. Sun\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e11. Osmometric Measurements of Cryoprotective Agent Permeation into Tissues\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Kezhou Wu, Leila Laouar, Nadia Shardt, Janet A.W. Elliott, and Nadr M. Jomha\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e12. Use of X-Ray Computed Tomography for Monitoring Tissue Permeation Processes\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Ariadna Corral, Alberto Olmo, and Ramón Risco\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e13. Use of In Situ Fourier Transform Infrared Spectroscopy in Cryobiological Research\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Willem F. Wolkers and Harriëtte Oldenhof\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e14. Raman Cryomicroscopic Imaging and Sample Holder for Spectroscopic Subzero Temperature Measurements\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Guanglin Yu, Rui Li, and Allison Hubel\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003ePart III: Cryopreservation and Freeze-Drying Protocols\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e15. Cryopreservation of Semen from Domestic Livestock: Bovine, Equine, and Porcine Sperm\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Harriëtte Oldenhof, Willem F. Wolkers, and Harald Sieme\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e16. Cryopreservation of Avian Semen\u003c\/b\u003e\u003c\/p\u003e              Henri Woelders\u003cp\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e17. Cryopreservation of Mouse Sperm for Genome Banking\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Yuksel Agca and Cansu Agca\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e18. Cryopreservation of Marine Invertebrates: From Sperm to Complex Larval Stages\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Estefania Paredes, Pablo Heres, Catarina Anjos, and Elsa Cabrita\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e19. Aseptic Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into a Cooling Agent\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Mengying Wang, Evgenia Isachenko, Gohar Rahimi, Peter Mallmann, and Vladimir Isachenko\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e20. Cryopreservation of Mammalian Oocytes: Slow Cooling and Vitrification as Successful Methods for Cryogenic Storage\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Victoria Keros and Barry J. Fuller\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e21.\u003c\/b\u003e\u003cb\u003e Vitrification of Porcine Oocytes and Zygotes in Microdrops on a Solid Metal Surface or Liquid Nitrogen\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Tamas Somfai and Kazuhiro Kikuchi\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e22. Cryopreservation and Transplantation of Laboratory Rodent Ovarian Tissue for Genome Banking and Biomedical Research\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Yuksel Agca and Cansu Agca\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cb\u003e23. Cryopreservation and Thawing of Human Ovarian Cortex Tissue Slices\u003c\/b\u003e\u003cp\u003e\u003c\/p\u003e  \u003cp\u003e            Jana Liebenthron and Markus Montag\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e24. Vitrification: A Simple and Successful Method for Cryostorage of Human Blastocysts\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Juergen Liebermann\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e25. Vitrification of Equine In Vivo-Derived Embryos, after Blastocoel Aspiration\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Carolina Herrera\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e26. Frozen Blood Reserves\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Johan W. Lagerberg\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e27. Isolation, Cryopreservation, and Characterization of iPSC-Derived Megakaryocytes\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Denys Pogozhykh, Rainer Blasczyk, and Constança Figueiredo\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e28. Chemically Defined, Clinical-Grade Cryopreservation of Human Adipose Stem Cells\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Melany López and Ali Eroglu\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e29. Chemically-Defined and Xeno-Free Cryopreservation of Human Induced Pluripotent Stem Cells\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Juliette Seremak and Ali Eroglu\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e30. Protocol for Cryopreservation of Endothelial Monolayers\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Leah A. Marquez-Curtis, Nasim Eskandari, Locksley E. McGann, and Janet A.W. Elliott\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e31. Vitrification of Heart Valve Tissues\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Kelvin G.M. Brockbank, Zhenzhen Chen, Elizabeth D. Greene, and Lia H. Campbell\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e32. Cryopreservation of Algae\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Estefania Paredes, Angela Ward, Ian Probert, Léna Gouhier, and Christine N. Campbell\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e33. Cryopreservation of Fern Spores and Pollen\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Anna Nebot, Victoria J. Philpott, Anna Pajdo, and Daniel Ballesteros\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e34. Cryopreservation of Plant Cell Lines Using Alginate Encapsulation\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Heinz Martin Schumacher, Martina Westphal, and Elke Heine-Dobbernack\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e35. Cryopreservation of Plant Shoot Tips of Potato, Mint, Garlic, and Shallot Using Plant Vitrification Solution 3\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Angelika Senula and Manuela Nagel\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e36. Cryopreservation of Seeds and Seed Embryos in Orthodox, Intermediate, and Recalcitrant Seeded Species\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Daniel Ballesteros, Natalia Fanega-Sleziak, and Rachael Davies\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e37. Freeze-Drying of Proteins\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Baolin Liu and Xinli Zhou\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e38. Freeze-Drying of Lactic Acid Bacteria: A Stepwise Approach for Developing a Freeze-Drying Protocol Based on Physical Properties\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Fernanda Fonseca, Amélie Girardeau, and Stéphanie Passot\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e39. Preservation of Mammalian Sperm by Freeze-Drying\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Levent Keskintepe and Ali Eroglu\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e40. Freeze-Drying of Decellularized Heart Valves for Off-the-Shelf Availability\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Willem F. Wolkers and Andres Hilfiker\u003c\/p\u003e","brand":"Springer-Verlag New York Inc.","offers":[{"title":"Default Title","offer_id":49406771167575,"sku":"9781071607824","price":161.99,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9781071607824.jpg?v=1730497053","url":"https:\/\/bookcurl.com\/products\/cryopreservation-and-freezedrying-protocols-9781071607824","provider":"Book Curl","version":"1.0","type":"link"}