{"product_id":"craniofacial-development-9781071618462","title":"Craniofacial Development","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003e\u003cp\u003eThis volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic\/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (\u003ci\u003ec. elegans\u003c\/i\u003e, zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant and \u003ci\u003ein vivo\u003c\/i\u003e cell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression using \u003ci\u003ein-situ \u003c\/i\u003ehybridisation and single-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such as CRISPR\/Cas9-mediated deletion. Written in the format of the highly successful \u003ci\u003eMethods in Molecular Biology \u003c\/i\u003eseries, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes ti\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e1. Using \u003ci\u003eCaenorhabditis elegans\u003c\/i\u003e as a model for mechanistic insights of craniofacial development\u003c\/p\u003e  \u003cp\u003eMichael Gruss and Ann K. Corsi\u003c\/p\u003e   2. Wholemount In-situ Hybridisation (WISH) in Zebrafish Embryos to Analyse Craniofacial DevelopmentNishanthi Mathiyalagan and Sebastian Dworkin\u003csup\u003e\u003c\/sup\u003e  \u003cp\u003e3. Elucidation of Gene Expression Patterns in The Craniofacial Tissues Of Mouse Embryos By Whole-Mount In Situ Hybridization\u003c\/p\u003e  \u003cp\u003eJoshua B Studdert, Heidi Bildsoe, V Pragathi Masamsetti, and Patrick P L Tam\u003csup\u003e\u003c\/sup\u003e\u003c\/p\u003e   \u003cp\u003e\u003c\/p\u003e  \u003cp\u003e4. Visualization of the Cartilage and Bone Elements in The Craniofacial Structures by Alcian blue and Alizarin Red Staining\u003c\/p\u003e  \u003cp\u003eJoshua B Studdert, Heidi Bildsoe, V Pragathi Masamsetti, and Patrick P L Tam\u003csup\u003e\u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  5. Methodology for Free-Floating Organ Culture of Mid-Gestation Maxillary Primordial Tissue\u003cp\u003e\u003c\/p\u003e  \u003cp\u003eM. E. de Vries and S. Dworkin\u003csup\u003e\u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e\u003csup\u003e \u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e6. Methods of Palate Culture in Later Palatogenesis: Elevation, Horizontal Outgrowth, and Fusion\u003c\/p\u003e  \u003cp\u003eJack D. Morgan and Jeremy B. A. Green\u003csup\u003e\u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e7. Single cell RNA-seq: Cell Isolation and Data Analysis\u003c\/p\u003e  \u003cp\u003eVal Yianni and Paul T Sharpe\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e8. Generating Zebrafish RNA-less Mutant Alleles by Deleting Gene Promoters with CRISPR\/Cas9 \u003c\/p\u003e  \u003cp\u003ePriyanka Kumari, Morgan Sturgeon, Gregory Bonde, and Robert A. Cornell\u003csup\u003e\u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e\u003csup\u003e \u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e9. Craniofacial Phenomics: Three-Dimensional Assessment of the Size and Shape of Cranial and Dentofacial Structures\u003c\/p\u003e  \u003cp\u003eMustafa Mian, Jenny Tan, Robin Yong, Ruth Williams, Agatha Labrinidis, Peter J. Anderson, and Sarbin Ranjitkar\u003csup\u003e\u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e\u003csup\u003e \u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e10. Micro-CT-based Bone Microarchitecture Analysis of the Murine Skull\u003c\/p\u003e  \u003cp\u003eJenny Tan, Agatha Labrinidis, Ruth Williams, Mustafa Mian, Peter J. Anderson, and Sarbin Ranjitkar\u003csup\u003e\u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e11. Characterization of mammalian \u003ci\u003ein vivo\u003c\/i\u003e enhancers using mouse transgenesis and CRISPR genome editing\u003c\/p\u003e  \u003cp\u003eMarco Osterwalder, Stella Tran, Riana D. Hunter, Eman M. Meky, Kianna von Maydell, Anne N. Harrington, Janeth Godoy, Catherine S. Novak, Ingrid Plajzer-Frick, Yiwen Zhu, Jennifer A. Akiyama, Veena Afzal, Evgeny Z. Kvon, Len A. Pennacchio, Diane E. Dickel, and Axel Visel\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e12. Histological Techniques for Sectioning Bones of the Vertebrate Craniofacial Skeleton\u003c\/p\u003e  \u003cp\u003eDaniela M. Roth, Lakshmi Puttagunta, and Daniel Graf\u003c\/p\u003e  \u003cp\u003e13. Engineering Epithelial-Mesenchymal Microtissues to Study Cell-Cell Interactions in Development \u003c\/p\u003e  \u003cp\u003eJacob I. Reynolds, Ross A. Vitek, Peter G. Geiger, and Brian P. Johnson\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e14. Ex Vivo Culture of Human Cranial Suture Cells\u003c\/p\u003e  \u003cp\u003ePeter J. Anderson\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  15. Scaffolds for Use in Craniofacial Bone Regeneration\u003cp\u003e\u003c\/p\u003e  \u003cp\u003eKatherine R. Hixon, Christopher T. Eberlin, Meghana Pendyala, Angela Alarcon de la Lastra, and Scott A. Sell\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e16. Mandible Explant Assay for The Analysis of Meckel’s Cartilage Development\u003c\/p\u003e  \u003cp\u003eSophie Wiszniak and Quenten Schwarz\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e17. High Resolution Histology for Craniofacial Studies On Zebrafish and Other Teleost Models\u003c\/p\u003e  A. Huysseune, M. Soenens, J.-Y. Sire, and P.E. Witten\u003cp\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e18. Live Imaging the Dynamics Of Mammalian Neural Crest Cell Migration\u003c\/p\u003e  \u003cp\u003eEmma L. Moore and Paul A. Trainor\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  19. Salivary Gland Development In Culture Marcia Gaete, Tathyane H. N. Teshima, Lemonia Chatzeli, and Abigail S. Tucker \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e20. Antenatal Ultrasound Imaging for Analysis of Human Craniosynostosis\u003c\/p\u003e  \u003cp\u003eSarah Constantine and Piotr Niznik\u003csup\u003e\u003c\/sup\u003e\u003c\/p\u003e","brand":"Springer-Verlag New York Inc.","offers":[{"title":"Default Title","offer_id":53186413592919,"sku":"9781071618462","price":161.99,"currency_code":"GBP","in_stock":true}],"url":"https:\/\/bookcurl.com\/products\/craniofacial-development-9781071618462","provider":"Book Curl","version":"1.0","type":"link"}