{"product_id":"coupled-bioluminescent-assays-9780470108833","title":"Coupled Bioluminescent Assays","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003eThis book highlights the applications of coupled bioluminescence assay techniques to real-world problems in drug discovery, environmental and chemical analysis, and biodefense. It separates theoretical aspects from the applied sections in a clear and readable way. Coupled Bioluminescent Assays, explains the uses of CB technologies across drug discovery to analyze toxicity, drug receptors, and enzymes. It covers applications in environmental analysis and biodefense, including cytotoxicity, fertilizer and explosives analysis, and nerve agent and pesticide detection. This is the premier reference on coupled bioluminescent assays for chemists, biochemists, and molecular biologists.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTrade Review\u003c\/b\u003e\u003cbr\u003e\u003cp\u003e\"The chapters of the book are well balanced.... Recommended to scientist and students who want to improve their knowledge of bioluminescent coupled assays and bioluminescence in general.\" (\u003ci\u003eAnalytical and Bioanalytical Chemistry\u003c\/i\u003e, August 2009)\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cb\u003ePREFACE.\u003c\/b\u003e  \u003cp\u003e\u003cb\u003ePART I BACKGROUND TO COUPLED BIOLUMINESCENT ASSAYS.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Introduction.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1.1 Introduction to Coupled Bioluminescent Assays.\u003c\/p\u003e \u003cp\u003e1.2 Luminescent Technologies of the Life Sciences.\u003c\/p\u003e \u003cp\u003e1.3 Varieties of Fluorometric Assays.\u003c\/p\u003e \u003cp\u003e1.4 Chemiluminescence and Bioluminescence.\u003c\/p\u003e \u003cp\u003e1.5 Common Bioluminescence Systems.\u003c\/p\u003e \u003cp\u003e1.6 A Coupled Bioluminescent Reaction.\u003c\/p\u003e \u003cp\u003e1.7 Summary.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Coupled Bioluminescent Reactions in Practice.\u003cbr\u003e \u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 Principles of Coupled Bioluminescent Reactions.\u003c\/p\u003e \u003cp\u003e2.2 Instrumentation and Equipment for Coupled Bioluminescent Assays.\u003c\/p\u003e \u003cp\u003e2.3 Coupled Bioluminescent Assay Procedures and Precautions.\u003c\/p\u003e \u003cp\u003e2.4 Data Handling for Coupled Bioluminescent Assays.\u003c\/p\u003e \u003cp\u003e2.5 Comparison of Coupled Bioluminescent Assays with Other Methods.\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePART II BIOMEDICAL APPLICATIONS OF COUPLED BIOLUMINESCENCE.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Coupled Bioluminescent Cytotoxicity Assays.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 Introduction.\u003c\/p\u003e \u003cp\u003e3.2 Membrane Integrity Assays.\u003c\/p\u003e \u003cp\u003e3.3 A Coupled Bioluminescent Assay for Enzyme Release: The G3PDH-Release Assay.\u003c\/p\u003e \u003cp\u003e3.4 Viability Assays.\u003cbr\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 The Role of Coupled Bioluminescent Assays in Kinase Screening and Study.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 The Many Roles of Kinases in Biology.\u003c\/p\u003e \u003cp\u003e4.2 Current Standard Kinase Assays.\u003c\/p\u003e \u003cp\u003e4.3 Coupled Bioluminescent Kinase Assays.\u003c\/p\u003e \u003cp\u003e4.4 Conclusions.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Coupled Bioluminescent Phosphatase Assays.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Introduction.\u003c\/p\u003e \u003cp\u003e5.2 Phosphatases.\u003c\/p\u003e \u003cp\u003e5.3 Contemporary Phosphatase Assay Technologies.\u003c\/p\u003e \u003cp\u003e5.4 CB Phosphatase Assays.\u003c\/p\u003e \u003cp\u003e5.5 Conclusions.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Acetylcholinesterase.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e6.1 Introduction.\u003c\/p\u003e \u003cp\u003e6.2 Established AChE Assay Methods.\u003c\/p\u003e \u003cp\u003e6.3 Recent Developments in AChE Assay Methods.\u003c\/p\u003e \u003cp\u003e6.4 Evaluation of Current AChE High-Throughput Screening Methods.\u003cbr\u003e \u003c\/p\u003e \u003cp\u003e6.5 Coupled Bioluminescent Assays of AChE Activity.\u003c\/p\u003e \u003cp\u003e6.6 Comparison of Coupled Bioluminescent and Other Methods of Measuring AChE Activity.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Measurement of Nitric Oxide Synthase Activity by Coupled Bioluminescence.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction.\u003c\/p\u003e \u003cp\u003e7.2 Current NOS Assays.\u003c\/p\u003e \u003cp\u003e7.3 Conclusions.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 The Coupled Bioluminescent Pyrophosphorolysis Assay.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8.1 Introduction.\u003c\/p\u003e \u003cp\u003e8.2 Genetic Variation in Modern Medicine.\u003c\/p\u003e \u003cp\u003e8.3 DNA Complementarity.\u003c\/p\u003e \u003cp\u003e8.4 The READIT® Pyrophosphorolysis Assay.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Coupled Luminescent Assays of G-Protein-Coupled Receptors.\u003cbr\u003e \u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e9.1 Introduction to G-Protein-Coupled Receptors.\u003c\/p\u003e \u003cp\u003e9.2 GPCR Assay Methods.\u003c\/p\u003e \u003cp\u003e9.3 Summary.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Coupled Bioluminescent Protease Assays.\u003cbr\u003e \u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e10.1 Introduction.\u003c\/p\u003e \u003cp\u003e10.2 Protease Assays.\u003c\/p\u003e \u003cp\u003e10.3 Coupled Bioluminescent Protease Assays.\u003c\/p\u003e \u003cp\u003e10.4 Summary.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Coupled Luminescent Assays Involving Aequorin.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e11.1 Introduction to Aequorin.\u003c\/p\u003e \u003cp\u003e11.2 Detection of Calcium in Practice.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Coupled Bioluminescent Reporter Assays.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e12.1 Introduction to Reporter Assays.\u003c\/p\u003e \u003cp\u003e12.2 Luciferases as Reporters of Promoter Activities.\u003c\/p\u003e \u003cp\u003e12.3 Aequorin as a Reporter Enzyme.\u003c\/p\u003e \u003cp\u003e12.4 Vectors for Use in Reporter Assays.\u003c\/p\u003e \u003cp\u003e12.5 Summary.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Coupled Bioluminescent Assays: Regulatory Concerns.\u003cbr\u003e \u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e13.1 Introduction.\u003c\/p\u003e \u003cp\u003e13.2 Regulatory Aspects of Assay Development.\u003c\/p\u003e \u003cp\u003e13.3 Summary.\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePART III OTHER APPLICATIONS OF COUPLED BIOLUMINESCENCE.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14 Coupled Bioluminescent Determination of Bioburden and Sterility.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e14.1 Introduction.\u003c\/p\u003e \u003cp\u003e14.2 Rapid Methods of Bioburden and Sterility Assessment.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15 Environmental Applications of Coupled Bioluminescent Assays.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e15.1 Introduction.\u003c\/p\u003e \u003cp\u003e15.2 Current Methods for Environmental Monitoring of Water Quality.\u003c\/p\u003e \u003cp\u003e15.3 Methods of Monitoring Stream Water and Lake Water.\u003c\/p\u003e \u003cp\u003e15.4 Methods of Monitoring Drinking Water.\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendix A: One-Letter Amino Acid Abbreviations.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eGlossary.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eBibliography.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eIndex.\u003c\/b\u003e\u003c\/p\u003e","brand":"John Wiley \u0026 Sons Inc","offers":[{"title":"Default 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