{"product_id":"cloning-gene-expression-and-protein-purification-9780195132946","title":"Cloning Gene Expression and Protein Purification","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003eThe central purpose of this combination lecture\/laboratory manual is to present detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing the encoded protein, purifying the protein, and characterizing rudimentary aspects of its basic physical properties. The manual includes 20 experiments designed to train students to prepare, manipulate, and analyse plasmids, to produce fusion proteins in bacteria, and to purify these proteins based on unique chemical properties or substrate affinities. Electrophoresis, Southern and Western blotting and cominatorial techniques are emphasized. Radioisotopes, fluorophores and solvent effects on protein structure are discussed. (No radioisotopes are required.)  The experiments included have been selected because of their high success rate and because they emphasize a project-oriented approach. Sufficient detail and background are provided such that the intended audience includes advanced undergr\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003eINTRODUCTORY UNIT; PART I - NCULEIC ACIDS \u0026amp; CLONING; UNIT 1; UNIT 2; UNIT 3; UNIT 4; UNIT 5; UNIT 6; PART 2 - PROTEIN PURIFICATION; UNIT 7; UNIT 8; UNIT 9; UNIT 10; APPENDICES; INDEX","brand":"Oxford University Press Inc","offers":[{"title":"Default Title","offer_id":48864209961303,"sku":"9780195132946","price":70.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9780195132946.jpg?v=1791454177","url":"https:\/\/bookcurl.com\/products\/cloning-gene-expression-and-protein-purification-9780195132946","provider":"Book Curl","version":"1.0","type":"link"}