{"product_id":"cell-based-assays-for-high-throughput-screening-methods-and-protocols-9781603275446","title":"Cell-Based Assays for High-Throughput Screening: Methods and Protocols","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003e\u003cp\u003eAs the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes ever more pressing. \u003cem\u003eCell-Based Assays for High-Throughput Screening: Methods and Protocols\u003c\/em\u003e aims to fill an important part of this need by providing an easily accessible reference volume for cell-based phenotypic screening. Leading researchers in the field contribute state-of-the-art methods with actionable protocols covering four major areas of study: model biological systems, screening modalities and assay systems, detection technologies, and approaches to data analysis. Written in the highly successful \u003cem\u003eMethods in Molecular Biology\u003c\/em\u003e™ series format, each chapter includes a brief introduction to the subject, lists of necessary materials and reagents, step-by-step laboratory protocols, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e \u003cp\u003eCutting-edge and easy-to-use, \u003cem\u003eCell-Based Assays for High-Throughput Screening: Methods and Protocols\u003c\/em\u003e presents an overview of relevant approaches, enabling the direct application of existing methods to new discoveries while also inspiring researchers to approach their screening projects in a conceptually modular fashion, enhancing the power to discover through new combinations of existing approaches.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTrade Review\u003c\/b\u003e\u003cbr\u003e\u003cp\u003eFrom the reviews:\u003c\/p\u003e \u003cp\u003e\"‘Cell-based assays for high-throughput screening’ is a highly practical resource, written by experts in each of the techniques … . very useful from a practical point of view, and anyone looking to find a new assay system will find a wealth of ideas here. … aimed at molecular biologists, many other scientists - such as natural product chemists looking for new lead molecules - should find it equally useful.\" (Elizabeth Williamson, Phytotherapy Research, September, 2009)\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003eChapter 1: Introduction: Cell-based Assays for High-throughput Screening W. Frank An and Nicola J. Tolliday  Chapter 2: High-throughput Screening of Model Bacteria Soumaya Zlitni, Jan E. Blanchard, and Eric D. Brown  Chapter 3: Cell-based Assays to Probe the ERK MAP Kinase Pathway in Endothelial Cells Michael R. Wyler, Deborah H. Smith, Eftihia Cayanis, Udo Toebben, Nathalie Aulner, and Thomas Mayer  Chapter 4: Large-scale Small-molecule Screen Using Zebrafish Embryos Charles C. Hong  Chapter 5: Whole-animal High-throughput Screens: The C. elegans Model Eyleen J. O’Rourke, Annie L. Conery, and Terence I. Moy  Chapter 6: Whole-organism Screening: Plants April Agee and David Carter  Chapter 7: Fluorescence-based Assays W. Frank An  Chapter 8: Reporter-gene Assays Andy M. F. Liu, David C. New, Rico K. H. Lo, and Yung H. Wong  Chapter 9: Screening for Chemical Inhibitors of Heterologous Proteins Expressed in Yeast Using a Simple Growth-restoration Assay Aruna D. Balgi and Michel Roberge  Chapter 10: Assay for Isolation of Inhibitors of Her2-kinase Expression Gabriela Chiosis and Adam B. Keeton  Chapter 11: ImageJ: A Primer Katherine Luby-Phelps  Chapter 12: High-content Screening: Flow Cytometry Analysis Bruce S. Edwards, Susan M. Young, Irena Ivnitsky-Steele, Richard D. Ye, Eric R. Prossnitz, and Larry A. Sklar  Chapter 13: High-throughput Real-time PCR for Detection of Gene-expression Levels Bridget K. Wagner and Zoltan Arany  Chapter 14: Interpretation of Uniform-well Readouts Serene Josiah  Chapter 15: Extracting Rich Information from Images Anne E. Carpenter","brand":"Humana Press Inc.","offers":[{"title":"Default Title","offer_id":53188817224023,"sku":"9781603275446","price":80.99,"currency_code":"GBP","in_stock":false}],"url":"https:\/\/bookcurl.com\/products\/cell-based-assays-for-high-throughput-screening-methods-and-protocols-9781603275446","provider":"Book Curl","version":"1.0","type":"link"}