{"product_id":"bioprocessing-technology-for-production-of-biopharmaceuticals-and-bioproducts-9781118361986","title":"Bioprocessing Technology for Production of","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003eIn the wake of rapid advances in genetic technologies, new products continue to be developed to help improve human health and quality of life.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cp\u003eList of Contributors xi\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart I Case Study 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Bacillus and the Story of Protein Secretion and Production 3\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eGiulia Barbieri, Anthony Calabria, Gopal Chotani, and Eugenio Ferrari\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e1.1 Bacillus as a Production Host: Introduction and Historical Account 3\u003c\/p\u003e \u003cp\u003e1.2 The Building of a Production Strain: Genetic Tools for B. subtilis Manipulation 5\u003c\/p\u003e \u003cp\u003e1.2.1 Promoters 5\u003c\/p\u003e \u003cp\u003e1.2.2 Vectors for Building a Production Strain 6\u003c\/p\u003e \u003cp\u003e1.2.3 B. subtilis Competent Cell Transformation 7\u003c\/p\u003e \u003cp\u003e1.2.4 Protoplasts-Mediated Manipulations 9\u003c\/p\u003e \u003cp\u003e1.2.5 Genetics by Electroporation 9\u003c\/p\u003e \u003cp\u003e1.3 B. subtilis Secretion Systemand Heterologous Protein Production 9\u003c\/p\u003e \u003cp\u003e1.3.1 Bacillus Fermentation and Recovery of Industrial Enzyme 11\u003c\/p\u003e \u003cp\u003e1.3.2 Fermentation Stoichiometry 12\u003c\/p\u003e \u003cp\u003e1.3.3 Fermentor Kinetics and Outputs 14\u003c\/p\u003e \u003cp\u003e1.3.4 Downstream Processing 17\u003c\/p\u003e \u003cp\u003e1.4 Summary 21\u003c\/p\u003e \u003cp\u003eReferences 21\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 New Expression Systems for GPCRs 29\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eDimitra Gialama, Fragiskos N. Kolisis, and Georgios Skretas\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e2.1 Introduction 29\u003c\/p\u003e \u003cp\u003e2.2 Recombinant GPCR Production – Traditional Approaches for Achieving High-Level Production 39\u003c\/p\u003e \u003cp\u003e2.3 Engineered Expression Systems for GPCR Production 42\u003c\/p\u003e \u003cp\u003e2.3.1 Bacteria 42\u003c\/p\u003e \u003cp\u003e2.3.2 Yeasts 48\u003c\/p\u003e \u003cp\u003e2.3.3 Insect Cells 51\u003c\/p\u003e \u003cp\u003e2.3.4 Mammalian Cells 54\u003c\/p\u003e \u003cp\u003e2.3.5 Transgenic Animals 54\u003c\/p\u003e \u003cp\u003e2.3.6 Cell-Free Systems 56\u003c\/p\u003e \u003cp\u003e2.4 Conclusion 57\u003c\/p\u003e \u003cp\u003eReferences 58\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Glycosylation 71\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eMaureen Spearman, Erika Lattová, Hélène Perreault, andMichael Butler\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e3.1 Introduction 71\u003c\/p\u003e \u003cp\u003e3.2 Types of Glycosylation 72\u003c\/p\u003e \u003cp\u003e3.2.1 N-linked Glycans 72\u003c\/p\u003e \u003cp\u003e3.2.2 O-linked Glycans 74\u003c\/p\u003e \u003cp\u003e3.3 Factors Affecting Glycosylation 76\u003c\/p\u003e \u003cp\u003e3.3.1 Nutrient Depletion 76\u003c\/p\u003e \u003cp\u003e3.3.2 Fed-batch Cultures and Supplements 79\u003c\/p\u003e \u003cp\u003e3.3.3 Specific Culture Supplements 80\u003c\/p\u003e \u003cp\u003e3.3.4 Ammonia 82\u003c\/p\u003e \u003cp\u003e3.3.5 pH 82\u003c\/p\u003e \u003cp\u003e3.3.6 Oxygen 83\u003c\/p\u003e \u003cp\u003e3.3.7 Host Cell Systems 83\u003c\/p\u003e \u003cp\u003e3.3.8 Other Factors 85\u003c\/p\u003e \u003cp\u003e3.4 Modification of Glycosylation 86\u003c\/p\u003e \u003cp\u003e3.4.1 siRNA and Gene Knockout\/Knockin 86\u003c\/p\u003e \u003cp\u003e3.4.2 Glycoprotein Processing Inhibitors and In Vitro Modification of Glycans 88\u003c\/p\u003e \u003cp\u003e3.5 Glycosylation Analysis 89\u003c\/p\u003e \u003cp\u003e3.5.1 Release of Glycans from Glycoproteins 90\u003c\/p\u003e \u003cp\u003e3.5.2 Derivatization of Glycans 91\u003c\/p\u003e \u003cp\u003e3.6 Methods of Analysis 91\u003c\/p\u003e \u003cp\u003e3.6.1 Lectin Arrays 91\u003c\/p\u003e \u003cp\u003e3.6.2 Liquid Chromatography 93\u003c\/p\u003e \u003cp\u003e3.6.2.1 HILIC Analysis 93\u003c\/p\u003e \u003cp\u003e3.6.2.2 Reversed Phase (RP) and Porous Graphitic Carbon (PGC) Chromatography 95\u003c\/p\u003e \u003cp\u003e3.6.2.3 Weak Anion Exchange (WAX) HPLC Analysis 96\u003c\/p\u003e \u003cp\u003e3.6.2.4 High pH Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD) 96\u003c\/p\u003e \u003cp\u003e3.6.3 Capillary Electrophoresis (CE) 97\u003c\/p\u003e \u003cp\u003e3.6.4 Fluorophore-assisted Carbohydrate Electrophoresis (FACE) and CGE-LIF 99\u003c\/p\u003e \u003cp\u003e3.6.5 Mass Spectrometry (MS) 100\u003c\/p\u003e \u003cp\u003e3.6.5.1 Ionization 100\u003c\/p\u003e \u003cp\u003e3.6.5.2 Derivatization Techniques Used for MS Analysis of Glycans 102\u003c\/p\u003e \u003cp\u003e3.6.5.3 Fragmentation of Carbohydrates 103\u003c\/p\u003e \u003cp\u003e3.7 Conclusion 109\u003c\/p\u003e \u003cp\u003eReferences 109\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart II Bioreactors 131\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Bioreactors for StemCell and Mammalian Cell Cultivation 133\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eAna Fernandes-Platzgummer, Sara M. Badenes, Cláudia L. da Silva, and JoaquimM. S. Cabral\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e4.1 Overview of (Mammalian and Stem) Cell Culture Engineering 133\u003c\/p\u003e \u003cp\u003e4.1.1 Cell Products for Therapeutics 134\u003c\/p\u003e \u003cp\u003e4.1.2 Cell as a Product: Stem Cells 136\u003c\/p\u003e \u003cp\u003e4.2 Bioprocess Characterization 140\u003c\/p\u003e \u003cp\u003e4.2.1 Cell Cultivation Methods 140\u003c\/p\u003e \u003cp\u003e4.2.2 Cell Metabolism 141\u003c\/p\u003e \u003cp\u003e4.2.3 Culture Medium Design 143\u003c\/p\u003e \u003cp\u003e4.2.4 Culture Parameters 144\u003c\/p\u003e \u003cp\u003e4.2.5 Culture Modes 145\u003c\/p\u003e \u003cp\u003e4.3 Cell Culture Systems 147\u003c\/p\u003e \u003cp\u003e4.3.1 Static Culture Systems 147\u003c\/p\u003e \u003cp\u003e4.3.2 Roller Bottles 150\u003c\/p\u003e \u003cp\u003e4.3.3 Spinner Flask 150\u003c\/p\u003e \u003cp\u003e4.3.4 Airlift Bioreactor 151\u003c\/p\u003e \u003cp\u003e4.3.5 Fixed\/Fluidized-Bed Bioreactor 152\u003c\/p\u003e \u003cp\u003e4.3.6 Wave Bioreactor 152\u003c\/p\u003e \u003cp\u003e4.3.7 Rotating-Wall Vessel Bioreactor 154\u003c\/p\u003e \u003cp\u003e4.3.8 Stirred Tank Bioreactor 155\u003c\/p\u003e \u003cp\u003e4.3.8.1 Agitation\/Shear Stress 156\u003c\/p\u003e \u003cp\u003e4.4 Cell Culture Modeling 157\u003c\/p\u003e \u003cp\u003e4.5 Case Studies 159\u003c\/p\u003e \u003cp\u003e4.5.1 Antibody Production in Bioreactor Systems 159\u003c\/p\u003e \u003cp\u003e4.5.2 mESC Expansion on Microcarriers in a Stirred Tank Bioreactor 161\u003c\/p\u003e \u003cp\u003e4.6 Concluding Remarks 162\u003c\/p\u003e \u003cp\u003eList of Symbols 163\u003c\/p\u003e \u003cp\u003eReferences 164\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Model-Based Technologies Enabling Optimal Bioreactor Performance 175\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eRimvydas Simutis, Marco Jenzsch, and Andreas Lübbert\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e5.1 Introduction 175\u003c\/p\u003e \u003cp\u003e5.2 Basics 176\u003c\/p\u003e \u003cp\u003e5.2.1 Balances 176\u003c\/p\u003e \u003cp\u003e5.2.2 Model Identification 177\u003c\/p\u003e \u003cp\u003e5.2.3 Model-Based Process Optimization 178\u003c\/p\u003e \u003cp\u003e5.3 Examples 180\u003c\/p\u003e \u003cp\u003e5.3.1 Model-Based State Estimation 180\u003c\/p\u003e \u003cp\u003e5.3.1.1 Static Model Approach 180\u003c\/p\u003e \u003cp\u003e5.3.1.2 Dynamic Alternatives 183\u003c\/p\u003e \u003cp\u003e5.3.2 Optimizing Open Loop-Controlled Cultivations 184\u003c\/p\u003e \u003cp\u003e5.3.2.1 Robust Cultivation Profiles 184\u003c\/p\u003e \u003cp\u003e5.3.2.2 Evolutionary Modeling Approach 188\u003c\/p\u003e \u003cp\u003e5.3.3 Optimization by Model-Aided Feedback Control 190\u003c\/p\u003e \u003cp\u003e5.3.3.1 Improving the Basic Control 190\u003c\/p\u003e \u003cp\u003e5.3.3.2 Optimizing the Amount of Soluble Product 190\u003c\/p\u003e \u003cp\u003e5.3.4 CO2-Removal in Large-Scale Cell Cultures 194\u003c\/p\u003e \u003cp\u003e5.4 Conclusion 197\u003c\/p\u003e \u003cp\u003eReferences 198\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Monitoring and Control of Bioreactor: Basic Concepts and Recent Advances 201\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eJames Gomes, Viki Chopda, and Anurag S. Rathore\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e6.1 Introduction 201\u003c\/p\u003e \u003cp\u003e6.2 Challenges in Bioprocess Control 202\u003c\/p\u003e \u003cp\u003e6.2.1 Process Dynamics and Modeling 202\u003c\/p\u003e \u003cp\u003e6.2.2 Limits of Hardware and Software andTheir Integration 203\u003c\/p\u003e \u003cp\u003e6.2.3 Regulatory Aspects 204\u003c\/p\u003e \u003cp\u003e6.3 Basic Elements of Bioprocess Control 205\u003c\/p\u003e \u003cp\u003e6.3.1 Bioprocess Monitoring 205\u003c\/p\u003e \u003cp\u003e6.3.2 Parameter Estimators 205\u003c\/p\u003e \u003cp\u003e6.3.3 Bioprocess Modeling 206\u003c\/p\u003e \u003cp\u003e6.4 Current Practices in Bioprocess Control 208\u003c\/p\u003e \u003cp\u003e6.4.1 PID Control 208\u003c\/p\u003e \u003cp\u003e6.4.2 Model-Based Control 209\u003c\/p\u003e \u003cp\u003e6.4.3 Adaptive Control 211\u003c\/p\u003e \u003cp\u003e6.4.4 Nonlinear Control 214\u003c\/p\u003e \u003cp\u003e6.5 Intelligent Control Systems 217\u003c\/p\u003e \u003cp\u003e6.5.1 Fuzzy Control 217\u003c\/p\u003e \u003cp\u003e6.5.2 Neural Control 219\u003c\/p\u003e \u003cp\u003e6.5.3 Statistical Process Control 222\u003c\/p\u003e \u003cp\u003e6.5.4 Integrated and Plant-Wide Bioprocess Control 224\u003c\/p\u003e \u003cp\u003e6.5.5 Metabolic Control 225\u003c\/p\u003e \u003cp\u003e6.6 Summary 226\u003c\/p\u003e \u003cp\u003e6.7 Future Perspectives 227\u003c\/p\u003e \u003cp\u003eAcknowledgments 227\u003c\/p\u003e \u003cp\u003eReferences 227\u003c\/p\u003e \u003cp\u003ePart III Host Strain Technologies 239\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Metabolic Engineering for Biocatalyst Robustness to Organic Inhibitors 241\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eLiam Royce and Laura R. Jarboe\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction 241\u003c\/p\u003e \u003cp\u003e7.2 Mechanisms of Inhibition 243\u003c\/p\u003e \u003cp\u003e7.3 Mechanisms of Tolerance 245\u003c\/p\u003e \u003cp\u003e7.4 Membrane Engineering 246\u003c\/p\u003e \u003cp\u003e7.5 Evolutionary and Metagenomic Strategies for Increasing Tolerance 251\u003c\/p\u003e \u003cp\u003e7.6 Reverse Engineering of Improved Strains 254\u003c\/p\u003e \u003cp\u003e7.7 Concluding Remarks 255\u003c\/p\u003e \u003cp\u003eAcknowledgments 255\u003c\/p\u003e \u003cp\u003eReferences 255\u003c\/p\u003e \u003cp\u003eIndex 267\u003c\/p\u003e","brand":"John Wiley \u0026 Sons Inc","offers":[{"title":"Default Title","offer_id":49406859805015,"sku":"9781118361986","price":107.96,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9781118361986.jpg?v=1730497361","url":"https:\/\/bookcurl.com\/products\/bioprocessing-technology-for-production-of-biopharmaceuticals-and-bioproducts-9781118361986","provider":"Book Curl","version":"1.0","type":"link"}