{"product_id":"angiogenesis-9781071620588","title":"Angiogenesis","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003eThis detailed book builds on the foundation of lymphatic protocols with tools to aid in the interpretation of large datasets created by angiogenesis research. For example, this includes protocols to explore how transcriptional phenotyping can be achieved, both in terms of exploring tissue-specific heterogeneity to the use of database mining of existing datasets. Similarly, bioinformatic techniques run alongside protocols for transcript analysis, cell biology, pathology, as well as physiological and developmental angiogenesis models. Written for the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. \u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\u003cdiv\u003eAuthoritative and up-to-date, \u003ci\u003eAngiogenesis: Methods and Protocols\u003c\/i\u003e provides a meaningful compendium of practical approaches to angio\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003ePart I: Developmental Biology\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e1. The Embryonic Mouse Hindbrain Model to Study Sprouting Angiogenesis In Vivo\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Gabriela D’Amico and Christiana Ruhrberg\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e2. A Workflow to Track and Analyze Endothelial Migration during Vascular Development in Zebrafish Embryos Using Lightsheet Microscopy\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Yan Chen, Paul C. Evans, and Robert N. Wilkinson\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e3. \u003c\/b\u003e\u003cb\u003eAnalyzing Vessel Regression and Endothelial Apoptosis as a Component of Angiogenic Vessel Remodeling\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Lachlan Whitehead, Emma C. Watson, and Zoe L. Grant\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e4. Whole-Mount Immunofluorescence Protocol for 3D Imaging, Reconstruction, and Quantification of 4th Pharyngeal Arch Artery Formation\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Elena Ioannou and Christiana Ruhrberg\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e5. Quantifying and Characterizing Angiogenesis Using the Postnatal Mouse Retina\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Alessandro Fantin\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003ePart II: Adult Physiology and Pathology\u003c\/b\u003e\u003c\/p\u003e   \u003cp\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e6. Characterization of Lymphatic Vasculature Using Whole-Mount Immunostaining of Mouse Embryonic Dorsal Skin\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Nikita Ved and Jacinta Isabelle Kalisch-Smith\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e7. Analyzing Lymphatic Vessel Patterning in Adult Tissue\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Louise A. Johnson\u003c\/p\u003e   \u003cp\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e8. Transmission Electron Microscopy of Endothelium\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Zubair Ahmed Nizamundeen, Loubna ElSaboni, Grace Vetch, Jumana Karim, Chris R. Neal, Claire L. Allen, David O. Bates, and Kenton P. Arkill\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e9. Measurement of Revascularisation in the Hind Limb after Experimental Ischemia in Mice\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Sohni Ria Bhalla, Federica Riu, Maria J.C. Machado, and David O. Bates\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e10. \u003c\/b\u003e\u003cb\u003eImaging Blood Vessels and Lymphatics in Mouse Trachea Wholemounts\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Peter Baluk and Donald M. McDonald\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e11. Non-Invasive Measurement of Retinal Microvascular Permeability during Loss of Endothelial Quiescence\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Naseeb K. Malhi, David O. Bates, Kenton P. Arkill, and Claire L. Allen\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e12. An Ex Vivo Tissue Culture Method for Discovering Cell Dynamics Involved in Stromal Vascular Fraction Vasculogenesis Using the Mouse Mesentery\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Dima Majbour, Ariana D. Suarez-Martinez, Nicholas A. Hodges, Arinola O. Lampejo, Banks M. Lomel, Elijah W. Rice, Hulan Shang, Adam J. Katz, and Walter L. Murfee\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e13. Quantitative Three-Dimensional Analysis of the Lymphatic Vasculature in the Postnatal Mouse Heart\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Konstantinos Klaourakis, Paul R. Riley, and Joaquim Miguel Vieira\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e14. Quantifying Lymphatic Vessel Density in Human Tissue Samples\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Stewart G. Martin, Emad Rakha, and Sarah J. Storr\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e15. Quantifying Vascular Remodeling in the Mouse Spinal Cord\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            M.E. Da Vitoria Lobo, Lydia Hardowar, and Richard P. Hulse\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e16. \u003c\/b\u003e\u003cb\u003eQuantitative Methods to Assess Adipose Vasculature\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Martina Rudnicki, Alexandra Pislaru, and Tara L. Haas\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e17. Quantification of Angiogenesis in Laser Choroidal Neovascularization\u003c\/b\u003e\u003c\/p\u003e              Kathryn R. Green, Nicholas Beazley-Long, Amy P. Lynch, Claire L. Allen, David O. Bates, and Andrew V. Benest\u003cp\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003ePart III: Reducing Complexity: Growing and Studying Endothelial Cells Outside the Body\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e18. \u003c\/b\u003e\u003cb\u003eA Rapid Adaptable Method for Isolation of Endothelial Cells from Human Adipose Tissue\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Jacqueline Herold and Joanna Kalucka\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e19. Simple Gene Knockdown in Endothelial Cells Using Short Interfering RNA Oligonucleotides\u003c\/b\u003e\u003c\/p\u003e              Zarah B. Tabrizi, Kathryn R. Green, Amy P. Lynch, Nada S. Ahmed, Nicholas Beazley-Long, and Andrew V. Benest\u003cp\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e20. Transcription Factor Chromatin Immunoprecipitation in Endothelial Cells\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Philip Kitchen, Kevin Gaston, and Padma-Sheela Jayaraman\u003c\/p\u003e   \u003cp\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e21. In Vitro Co-Culture of Fibroblast and Endothelial Cells to Assess Angiogenesis\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Adam F. Odell and Aarren J. Mannion\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e22. Quantification of Leukocyte-Endothelial Cell Interaction during In Vitro Models of Thrombosis at Arterial and Venous Shear Rates\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Joshua H. Bourne\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e23. Isolation of Cardiac Endothelial Cells for Transcriptomic Analysis of the Zebrafish and Mouse Heart\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Ziwen Li, Katherine M. Ross Stewart, Finnius A. Bruton, Martin A. Denvir, and Mairi Brittan\u003c\/p\u003e   \u003cp\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e24. \u003c\/b\u003e\u003cb\u003eThe Evaluation of Neovessel Angiogenesis Behavior at Tissue Interfaces\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Hannah A. Strobel and James B. Hoying\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e25. Endothelial Cell Fibrin Gel Angiogenesis Bead Assay\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Eva Clavane, Hannah Taylor, Richard Cubbon, and Paul Meakin\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e26. Live Cell Imaging and Analysis of Cancer-Cell Transmigration through Endothelial Monolayers\u003c\/b\u003e\u003c\/p\u003e              Aarren J. Mannion\u003cp\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e27. Differentiation and Characterization of Human Pluripotent Stem Cell-Derived Cardiac Endothelial Cells for In Vitro Applications\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Kavita Raniga, Nguyen T.N. Vo, and Chris Denning\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003ePart IV: Making Use of Publically Existing Datasets and Resources\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e28. Finding and Verifying Enhancers for Endothelial-Expressed Genes\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Alice Neal, Helena Rodriguez-Caro, and Sarah De Val\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e29. \u003c\/b\u003e\u003cb\u003eEndothelial Cell RNA-Seq Data: Differential Expression and Functional Enrichment Analyses to Study Phenotypic Switching\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e            Guillermo Díez Pinel, Joseph L. Horder, John R. King, Alan McIntyre, Nigel P. Mongan, Gonzalo Gómez López, and Andrew V. Benest\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Springer-Verlag New York Inc.","offers":[{"title":"Default Title","offer_id":53186413527383,"sku":"9781071620588","price":224.99,"currency_code":"GBP","in_stock":true}],"url":"https:\/\/bookcurl.com\/products\/angiogenesis-9781071620588","provider":"Book Curl","version":"1.0","type":"link"}