{"product_id":"analysis-of-genes-and-genomes-9780470843802","title":"Analysis of Genes and Genomes","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003eA guide to the tools and techniques of genetic engineering, gene cloning and molecular biology. It covers various aspects of genetic engineering in the post-genomic era, beginning with the basics of DNA structure and DNA metabolism.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTrade Review\u003c\/b\u003e\u003cbr\u003e\"It could be an excellent supporting book to go along with a more general text in a course covering molecular biology.” (\u003ci\u003eBiochemistry and Molecular Education\u003c\/i\u003e, July\/ August 2004)  \u003cp\u003e\"...the list of relevant Nobel laureates at the end will be useful and was a delight…\" (Heredity, Vol. 94, 2005)\u003c\/p\u003e \u003cp\u003e\"…the full range of topics one would wish to see in such a book are covered…I certainly welcome the book…\" (Genetical Research, Vol.84, 2004)\u003c\/p\u003e \u003cp\u003e\" … invaluable to those looking to better understand the complexities and capabilities of these important new technologies.\" (\u003ci\u003eThaiszia Journal of Botany, Vol. 15; 173-185, 2005)\u003c\/i\u003e\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cp\u003ePreface xiii\u003c\/p\u003e \u003cp\u003eAcknowledgements xv\u003c\/p\u003e \u003cp\u003eAbbreviations and acronyms xvii\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 DNA: Structure and function 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1.1 Nucleic acid is the material of heredity 2\u003c\/p\u003e \u003cp\u003e1.2 Structure of nucleic acids 7\u003c\/p\u003e \u003cp\u003e1.3 The double helix 11\u003c\/p\u003e \u003cp\u003e1.3.1 The antiparallel helix 12\u003c\/p\u003e \u003cp\u003e1.3.2 Base pairs and stacking 14\u003c\/p\u003e \u003cp\u003e1.3.3 Gaining access to information with the double helix without breaking it apart 16\u003c\/p\u003e \u003cp\u003e1.3.4 Hydrogen bonding 17\u003c\/p\u003e \u003cp\u003e1.4 Reversible denaturing of DNA 18\u003c\/p\u003e \u003cp\u003e1.5 Structure of DNA in the cell 21\u003c\/p\u003e \u003cp\u003e1.6 The eukaryotic nucleosome 24\u003c\/p\u003e \u003cp\u003e1.7 The replication of DNA 28\u003c\/p\u003e \u003cp\u003e1.8 DNA polymerases 31\u003c\/p\u003e \u003cp\u003e1.9 The replication process 33\u003c\/p\u003e \u003cp\u003e1.10 Recombination 37\u003c\/p\u003e \u003cp\u003e1.11 Genes and genomes 39\u003c\/p\u003e \u003cp\u003e1.12 Genes within a genome 40\u003c\/p\u003e \u003cp\u003e1.13 Transcription 43\u003c\/p\u003e \u003cp\u003e1.13.1 Transcription in prokaryotes 43\u003c\/p\u003e \u003cp\u003e1.13.2 Transcription in eukaryotes 46\u003c\/p\u003e \u003cp\u003e1.14 RNA processing 54\u003c\/p\u003e \u003cp\u003e1.14.1 RNA splicing 55\u003c\/p\u003e \u003cp\u003e1.14.2 Alternative splicing 58\u003c\/p\u003e \u003cp\u003e1.15 Translation 59\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Basic techniques in gene analysis 65\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 Restriction enzymes 66\u003c\/p\u003e \u003cp\u003e2.1.1 Types of restriction–modification system 70\u003c\/p\u003e \u003cp\u003e2.1.2 Other modification systems 72\u003c\/p\u003e \u003cp\u003e2.1.3 How do type II restriction enzymes work? 74\u003c\/p\u003e \u003cp\u003e2.2 Joining DNA molecules 76\u003c\/p\u003e \u003cp\u003e2.3 The basics of cloning 78\u003c\/p\u003e \u003cp\u003e2.4 Bacterial transformation 84\u003c\/p\u003e \u003cp\u003e2.4.1 Chemical transformation 86\u003c\/p\u003e \u003cp\u003e2.4.2 Electroporation 87\u003c\/p\u003e \u003cp\u003e2.4.3 Gene gun 88\u003c\/p\u003e \u003cp\u003e2.5 Gel electrophoresis 88\u003c\/p\u003e \u003cp\u003e2.5.1 Polyacrylamide gels 89\u003c\/p\u003e \u003cp\u003e2.5.2 Agarose gels 89\u003c\/p\u003e \u003cp\u003e2.5.3 Pulsed-field gel electrophoresis 95\u003c\/p\u003e \u003cp\u003e2.6 Nucleic acid blotting 98\u003c\/p\u003e \u003cp\u003e2.6.1 Southern blotting 100\u003c\/p\u003e \u003cp\u003e2.6.2 The compass points of blotting 102\u003c\/p\u003e \u003cp\u003e2.7 DNA purification 103\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Vectors 109\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 Plasmids 112\u003c\/p\u003e \u003cp\u003e3.1.1 pBR 322 116\u003c\/p\u003e \u003cp\u003e3.1.2 pUC plasmids 119\u003c\/p\u003e \u003cp\u003e3.2 Selectable markers 122\u003c\/p\u003e \u003cp\u003e3.3 λ vectors 126\u003c\/p\u003e \u003cp\u003e3.4 Cosmid vectors 135\u003c\/p\u003e \u003cp\u003e3.5 M13 vectors 137\u003c\/p\u003e \u003cp\u003e3.6 Phagemids 140\u003c\/p\u003e \u003cp\u003e3.7 Artificial chromosomes 142\u003c\/p\u003e \u003cp\u003e3.7.1 YACs 143\u003c\/p\u003e \u003cp\u003e3.7.2 PACs 146\u003c\/p\u003e \u003cp\u003e3.7.3 BACs 148\u003c\/p\u003e \u003cp\u003e3.7.4 HACs 149\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Polymerase chain reaction 153\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 PCR reaction conditions 159\u003c\/p\u003e \u003cp\u003e4.2 Thermostable DNA polymerases 162\u003c\/p\u003e \u003cp\u003e4.3 Template DNA 164\u003c\/p\u003e \u003cp\u003e4.4 Oligonucleotide primers 165\u003c\/p\u003e \u003cp\u003e4.4.1 Synthesis of oligonucleotide primers 167\u003c\/p\u003e \u003cp\u003e4.5 Primer mismatches 169\u003c\/p\u003e \u003cp\u003e4.6 PCR in the diagnosis of genetic disease 173\u003c\/p\u003e \u003cp\u003e4.7 Cloning PCR products 175\u003c\/p\u003e \u003cp\u003e4.8 RT–PCR 177\u003c\/p\u003e \u003cp\u003e4.9 Real-time PCR 179\u003c\/p\u003e \u003cp\u003e4.10 Applications of PCR 181\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Cloning a gene 183\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Genomic libraries 185\u003c\/p\u003e \u003cp\u003e5.2 cDNA libraries 191\u003c\/p\u003e \u003cp\u003e5.3 Directional cDNA cloning 196\u003c\/p\u003e \u003cp\u003e5.4 PCR based libraries 199\u003c\/p\u003e \u003cp\u003e5.5 Subtraction libraries 200\u003c\/p\u003e \u003cp\u003e5.6 Library construction in the post-genome era 204\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Gene identification 205\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e6.1 Screening by nucleic acid hybridization 206\u003c\/p\u003e \u003cp\u003e6.2 Immunoscreening 211\u003c\/p\u003e \u003cp\u003e6.3 Screening by function 216\u003c\/p\u003e \u003cp\u003e6.4 Screening by interaction 217\u003c\/p\u003e \u003cp\u003e6.5 Phage display 218\u003c\/p\u003e \u003cp\u003e6.6 Two-hybrid screening 218\u003c\/p\u003e \u003cp\u003e6.6.1 Problems, and some solutions, with two-hybrid screening 225\u003c\/p\u003e \u003cp\u003e6.7 Other interaction screens – variations on a theme 228\u003c\/p\u003e \u003cp\u003e6.7.1 One hybrid 229\u003c\/p\u003e \u003cp\u003e6.7.2 Three hybrid 229\u003c\/p\u003e \u003cp\u003e6.7.3 Reverse two hybrid 229\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Creating mutations 231\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e7.1 Creating specific DNA changes using primer extension mutagenesis 233\u003c\/p\u003e \u003cp\u003e7.2 Strand selection methods 237\u003c\/p\u003e \u003cp\u003e7.2.1 Phosphorothioate strand selection 237\u003c\/p\u003e \u003cp\u003e7.2.2 dut − ung − (or Kunkel) strand selection 238\u003c\/p\u003e \u003cp\u003e7.3 Cassette mutagenesis 240\u003c\/p\u003e \u003cp\u003e7.4 PCR based mutagenesis 241\u003c\/p\u003e \u003cp\u003e7.5 QuikChange ® mutagenesis 248\u003c\/p\u003e \u003cp\u003e7.6 Creating random mutations in specific genes 250\u003c\/p\u003e \u003cp\u003e7.7 Protein engineering 254\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Protein production and purification 257\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8.1 Expression in E. coli 258\u003c\/p\u003e \u003cp\u003e8.1.1 The lac promoter 259\u003c\/p\u003e \u003cp\u003e8.1.2 The tac promoter 259\u003c\/p\u003e \u003cp\u003e8.1.3 The λP L promoter 260\u003c\/p\u003e \u003cp\u003e8.1.4 The T7 expression system 261\u003c\/p\u003e \u003cp\u003e8.2 Expression in yeast 265\u003c\/p\u003e \u003cp\u003e8.2.1 Saccharomyces cerevisiae 265\u003c\/p\u003e \u003cp\u003e8.2.1.1 The GAL system 266\u003c\/p\u003e \u003cp\u003e8.2.1.2 The CUP1 system 268\u003c\/p\u003e \u003cp\u003e8.2.2 Pichia pastoris 268\u003c\/p\u003e \u003cp\u003e8.2.3 Schizosaccharomyces pombe 269\u003c\/p\u003e \u003cp\u003e8.3 Expression in insect cells 269\u003c\/p\u003e \u003cp\u003e8.4 Expression in higher-Eukaryotic cells 272\u003c\/p\u003e \u003cp\u003e8.4.1 Tet-on\/Tet-off system 272\u003c\/p\u003e \u003cp\u003e8.5 Protein purification 275\u003c\/p\u003e \u003cp\u003e8.5.1 The His-tag 276\u003c\/p\u003e \u003cp\u003e8.5.2 The GST-tag 279\u003c\/p\u003e \u003cp\u003e8.5.3 The MBP-tag 282\u003c\/p\u003e \u003cp\u003e8.5.4 Impact 282\u003c\/p\u003e \u003cp\u003e8.5.5 TAP-tagging 286\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Genome sequencing projects 287\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e9.1 Genomic mapping 289\u003c\/p\u003e \u003cp\u003e9.2 Genetic mapping 290\u003c\/p\u003e \u003cp\u003e9.3 Physical mapping 293\u003c\/p\u003e \u003cp\u003e9.4 Nucleotide sequencing 295\u003c\/p\u003e \u003cp\u003e9.4.1 Manual DNA sequencing 296\u003c\/p\u003e \u003cp\u003e9.4.2 Automated DNA sequencing 300\u003c\/p\u003e \u003cp\u003e9.5 Genome sequencing 303\u003c\/p\u003e \u003cp\u003e9.6 The human genome project 305\u003c\/p\u003e \u003cp\u003e9.7 Finding genes 307\u003c\/p\u003e \u003cp\u003e9.8 Gene assignment 309\u003c\/p\u003e \u003cp\u003e9.9 Bioinformatics 311\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Post-genome analysis 313\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e10.1 Global changes in gene expression 314\u003c\/p\u003e \u003cp\u003e10.1.1 Differential display 315\u003c\/p\u003e \u003cp\u003e10.1.2 Microarrays 317\u003c\/p\u003e \u003cp\u003e10.1.3 ChIPs with everything 324\u003c\/p\u003e \u003cp\u003e10.2 Protein function on a genome-wide scale 327\u003c\/p\u003e \u003cp\u003e10.3 Knock-out analysis 327\u003c\/p\u003e \u003cp\u003e10.4 Antisense and RNA interference (RNAi) 329\u003c\/p\u003e \u003cp\u003e10.5 Genome-wide two-hybrid screens 333\u003c\/p\u003e \u003cp\u003e10.6 Protein detection arrays 335\u003c\/p\u003e \u003cp\u003e10.7 Structural genomics 335\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Engineering plants 341\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e11.1 Cloning in plants 341\u003c\/p\u003e \u003cp\u003e11.1.1 Agrobacterium tumefaciens 342\u003c\/p\u003e \u003cp\u003e11.1.2 Direct nuclear transformation 347\u003c\/p\u003e \u003cp\u003e11.1.3 Viral vectors 348\u003c\/p\u003e \u003cp\u003e11.1.4 Chloroplast transformation 350\u003c\/p\u003e \u003cp\u003e11.2 Commercial exploitation of plant transgenics 354\u003c\/p\u003e \u003cp\u003e11.2.1 Delayed ripening 354\u003c\/p\u003e \u003cp\u003e11.2.2 Insecticidal resistance 355\u003c\/p\u003e \u003cp\u003e11.2.3 Herbicidal resistance 356\u003c\/p\u003e \u003cp\u003e11.2.4 Viral resistance 357\u003c\/p\u003e \u003cp\u003e11.2.5 Fungal resistance 358\u003c\/p\u003e \u003cp\u003e11.2.6 Terminator technology 358\u003c\/p\u003e \u003cp\u003e11.3 Ethics of genetically engineered crops 360\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Engineering animal cells 361\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e12.1 Cell culture 361\u003c\/p\u003e \u003cp\u003e12.2 Transfection of animal cells 362\u003c\/p\u003e \u003cp\u003e12.2.1 Chemical transfection 363\u003c\/p\u003e \u003cp\u003e12.2.2 Electroporation 364\u003c\/p\u003e \u003cp\u003e12.2.3 Liposome-mediated transfection 364\u003c\/p\u003e \u003cp\u003e12.2.4 Peptides 366\u003c\/p\u003e \u003cp\u003e12.2.5 Direct DNA transfer 366\u003c\/p\u003e \u003cp\u003e12.3 Viruses as vectors 367\u003c\/p\u003e \u003cp\u003e12.3.1 SV 40 367\u003c\/p\u003e \u003cp\u003e12.3.2 Adenovirus 369\u003c\/p\u003e \u003cp\u003e12.3.3 Adeno-associated virus (AAV) 371\u003c\/p\u003e \u003cp\u003e12.3.4 Retrovirus 372\u003c\/p\u003e \u003cp\u003e12.4 Selectable markers and gene amplification in animal cells 375\u003c\/p\u003e \u003cp\u003e12.5 Expressing genes in animal cells 378\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Engineering animals 379\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e13.1 Pronuclear injection 381\u003c\/p\u003e \u003cp\u003e13.2 Embryonic stem cells 384\u003c\/p\u003e \u003cp\u003e13.3 Nuclear transfer 390\u003c\/p\u003e \u003cp\u003e13.4 Gene therapy 396\u003c\/p\u003e \u003cp\u003e13.5 Examples and potential of gene therapy 398\u003c\/p\u003e \u003cp\u003eGlossary 401\u003c\/p\u003e \u003cp\u003eProteins 409\u003c\/p\u003e \u003cp\u003eA. 1 409\u003c\/p\u003e \u003cp\u003eA1. 2 410\u003c\/p\u003e \u003cp\u003eA1. 3 411\u003c\/p\u003e \u003cp\u003eNobel prize winners 413\u003c\/p\u003e \u003cp\u003eReferences 417\u003c\/p\u003e \u003cp\u003eIndex 459\u003c\/p\u003e","brand":"John Wiley \u0026 Sons Inc","offers":[{"title":"Default Title","offer_id":49402435666263,"sku":"9780470843802","price":47.45,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9780470843802.jpg?v=1730480388","url":"https:\/\/bookcurl.com\/products\/analysis-of-genes-and-genomes-9780470843802","provider":"Book Curl","version":"1.0","type":"link"}