{"product_id":"african-swine-fever-virus-9781071623329","title":"African Swine Fever Virus","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003eThis volume provides a compilation of methods that will aid researchers studying virology, immunology, and vaccinology of this devastating disease of swine.Written in the format of the highly successfulMethods in Molecular Biologyseries, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.   Authoritative and cutting-edge,African Swine Fever Virus: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cp\u003e1               Working safely with African swine fever virus.\u003c\/p\u003e  Sarah Gold, Anna Lawton, and Graeme Harkess \u003cp\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e2               Necropsy procedures and evaluation of macroscopic lesions of pigs infected with African swine fever virus.\u003c\/p\u003e  \u003cp\u003ePedro J. Sánchez-Cordón, Fabian Lean, Matthieu Bernard, and Alejandro Núñez\u003c\/p\u003e   \u003cp\u003e\u003c\/p\u003e  \u003cp\u003e3               Preparation of immunofluorescently labelled tissue sections for imaging at low and high magnifications in the confocal microscope.\u003c\/p\u003e  \u003cp\u003eJennifer Simpson and Pippa Hawes \u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  4               Primary macrophage culture from porcine blood and lungs.\u003cp\u003e\u003c\/p\u003e  \u003cp\u003eLynnette C. Goatley, Rachel Nash, and Christopher L Netherton\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e5               Isolation of porcine bone marrow cells and generation of recombinant African swine fever viruses.\u003c\/p\u003e  \u003cp\u003eAnusyah Rathakrishnan, Ana Luisa Reis, Katy Moffat, and Linda K. Dixon \u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e6               Laboratory diagnosis and quantification of African swine fever virus using real-time polymerase chain reaction.\u003c\/p\u003e  Christopher L Netherton, Lynnette C Goatley, John Flannery, Martin Ashby, and Carrie Batten \u003cp\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e7               Quantification of ASFV DNA and RNA in Ornithodoros soft ticks\u003c\/p\u003e  \u003cp\u003eRémi Pereira De Oliveira, Laurence Vial, and Marie-Frédérique Le Potier\u003c\/p\u003e   \u003cp\u003e\u003c\/p\u003e  \u003cp\u003e8               Genotyping of African swine fever virus.\u003c\/p\u003e  \u003cp\u003ePaulina Rajko-Nenow and Carrie Batten\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e9               African swine fever virus (ASFV) indirect ELISA test based on the use of the soluble cytoplasmic semi purified antigen (ASFV CP-Ag).\u003c\/p\u003e  \u003cp\u003eCarmina Gallardo, Raquel Nieto, and Marisa Arias\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  10            Indirect immunoperoxidase test (IPT) for detection of antibodies against African swine fever virus (ASFV) on African green monkey cell lines (Vero, MS)\u003cp\u003e\u003c\/p\u003e  \u003cp\u003eCarmina Gallardo, Raquel Nieto, and Marisa Arias\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e11            African Swine Fever Virus Haemadosrption Inhibition Assay\u003c\/p\u003e  \u003cp\u003eAlexander Malogolovkin and Alexey Sereda\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e12            ELISpot assay for the detection of ASFV-specific interferon-gamma (IFN-γ) producing cells\u003c\/p\u003e  Raquel Portugal\u003cp\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e13            Purification of African swine fever virus.\u003c\/p\u003e  \u003cp\u003eGareth L. Shimmon, Pranav NM Shah, Elizabeth Fry, David I Stuart, Pippa Hawes and Christopher L. Netherton\u003csup\u003e\u003c\/sup\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e14            African swine fever virus plaque assay and disinfectant testing. \u003c\/p\u003e  Lorraine Frost and Carrie Batten\u003cp\u003e\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e15            Bioorthogonal labelling of African swine fever virus infected cells.\u003c\/p\u003e  \u003cp\u003eSophie-Marie Aicher\u003c\/p\u003e  \u003cp\u003e \u003c\/p\u003e  \u003cp\u003e16            Whole genome sequencing of African swine fever virus.\u003c\/p\u003e  Ha Thi Thanh Tran, Anh Duc Truong, and Hoang Vu Dang\u003cp\u003e\u003c\/p\u003e\u003cbr\u003e","brand":"Springer-Verlag New York Inc.","offers":[{"title":"Default Title","offer_id":53186413723991,"sku":"9781071623329","price":132.99,"currency_code":"GBP","in_stock":true}],"url":"https:\/\/bookcurl.com\/products\/african-swine-fever-virus-9781071623329","provider":"Book Curl","version":"1.0","type":"link"}