{"product_id":"advanced-technologies-for-protein-complex-production-and-characterization-9783319272146","title":"Advanced Technologies for Protein Complex","description":"\u003cb\u003eBook Synopsis\u003c\/b\u003e\u003cbr\u003e\u003cp\u003eThis book presents advanced expression technologies for the production of protein complexes. Since complexes lie at the heart of modern biology, the expression, purification, and characterization of large amounts of high-quality protein complexes is crucial for the fields of biomedicine, biotechnology, and structural biology. From co-expression in \u003ci\u003eE. coli\u003c\/i\u003e, yeast, mammalian and insect cells to complex reconstitution from individual subunits, this book offers useful insights and guidance for successful protein expressionists.\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003eAcross several sections readers will discover existing opportunities for the production of protein complexes in bacterial systems (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (\u003ci\u003eLeishmania terantolae\u003c\/i\u003e and \u003ci\u003eDictyostelium discoideum\u003c\/i\u003e), baculovirus-infected insect cells, mammalian cells, plants and algae. Complex reconstitution from individually purified subunits or subcomplexes is discussed as a complementary strategy. A last section introduces briefly some of the biophysical and structural characterization techniques for macromolecular complexes using state-of-the-art solution scattering and nuclear magnetic resonance.\u003c\/p\u003e  \u003cp\u003eThis work is a guided tour over some of the most powerful and successful protein expression technologies, with a focus on co-expression and high-throughput applications. It is addressed to everyone interested in the production and characterization of macromolecular complexes, from university students who want an accessible description of the major co-expression systems to researchers in biomedicine and the life sciences seeking for an up-to-date survey of available technologies.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTable of Contents\u003c\/b\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003ePreface. Complex Biologics: Engineering the Tools and the Hosts.\u003c\/b\u003e \u003ci\u003eM. Cristina Vega, Francisco J. Fernández and Imre Berger\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003eSection 1: Introduction\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e1\u003c\/b\u003e\u003cb\u003e.\u003c\/b\u003e Protein complex production from the drug discovery standpoint; \u003ci\u003eIsmail Moarefi\u003c\/i\u003e     \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e\u0026lt;2.\u003c\/b\u003e Choose a suitable expression host: a survey of available protein production platforms; \u003ci\u003eFrancisco J. Fernández and M. Cristina Vega\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003eSection 2: Prokaryotic expression hosts\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e3.\u003c\/b\u003e\u003cb\u003e \u003c\/b\u003eACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts; \u003ci\u003eYan Nie, Maxime Chaillet, Christian Becke, Matthias Haffke, Martin Pelosse, Daniel Fitzgerald, Ian Collinson, Christiane Schaffitzel and Imre Berger\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e4.\u003c\/b\u003e\u003cb\u003e \u003c\/b\u003eComplex reconstitution and characterization by combining co-expression techniques in \u003ci\u003eEscherichia coli\u003c\/i\u003e with high-throughput; \u003ci\u003eRenaud Vincentelli and Christoph Romier\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e5.\u003c\/b\u003e\u003cb\u003e \u003c\/b\u003eMembrane protein production in \u003ci\u003eE. coli\u003c\/i\u003e for applications in drug discovery; \u003ci\u003eHarm Jan (Arjan) Snijder and Jonna Hakulinen\u003c\/i\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e6.\u003c\/b\u003e\u003cb\u003e \u003c\/b\u003eCell-free Synthesis of Macromolecular Complexes; \u003ci\u003eMatthieu Botte, Aurélien Deniaud and Christiane Schaffitzel\u003c\/i\u003e                                                                                         \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e7.\u003c\/b\u003e\u003cb\u003e \u003c\/b\u003eA\u003ci\u003e Bacillus megaterium\u003c\/i\u003e system for the production of recombinant proteins and protein complexes; \u003ci\u003eRebekka Biedendieck\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e8.\u003c\/b\u003e\u003cb\u003e \u003c\/b\u003eProtein complex production in alternative prokaryotic hosts; \u003ci\u003eSara Gómez, Miguel López-Estepa, Francisco J. Fernández and M. Cristina Vega\u003c\/i\u003e                              \u003c\/p\u003e  \u003cp\u003e\u003cb\u003eSection 3: Lower Eukaryotic expression hosts\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e9.\u003cb\u003e \u003c\/b\u003eProduction of Protein Complexes in Non-methylotrophic and Methylotrophic Yeasts; \u003ci\u003eFrancisco J. Fernandez, Miguel López-Estepa, Javier Querol-García and M. Cristina Vega\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e10.\u003c\/b\u003e\u003cb\u003e \u003c\/b\u003e\u003ci\u003eLeishmania \u003c\/i\u003etarentolae for the Production of Multi-Subunit Complexes; \u003ci\u003eTomoaki Niimi\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003e11.\u003cb\u003e \u003c\/b\u003eAlternative Eukaryotic Expression Systems for the Production of Proteins and Protein Complexes; \u003ci\u003eSara Gómez, Miguel López-Estepa, Francisco J. Fernández, Teresa Suárez and M. Cristina Vega\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003eSection 4: Higher Eukaryotic expression hosts\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e12.\u003c\/b\u003e\u003cb\u003e \u003c\/b\u003eFundamentals of baculovirus expression and applications; \u003ci\u003eTom A. Kost and Christopher W. Kemp\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e13.\u003c\/b\u003e\u003cb\u003e \u003c\/b\u003eThe MultiBac Baculovirus \/ Insect Cell Expression Vector System for Producing Complex Protein Biologics; \u003ci\u003eDuygu Sari, Kapil Gupta, Deepak Balaji Govinda Raj, Alice Aubert, Petra Dmcova, Frederic Garzoni, Daniel Fitzgerald and Imre Berger\u003c\/i\u003e  \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e14. \u003c\/b\u003eFundamentals of expression in mammalian cells;\u003cb\u003e \u003c\/b\u003e\u003ci\u003eMichael R. Dyson\u003c\/i\u003e \u003c\/p\u003e  \u003cb\u003e15.\u003c\/b\u003e Assembling multi-subunit complexes using mammalian expression; \u003ci\u003eBahar Baser and Joop van den Heuvel\u003c\/i\u003e  \u003cp\u003e\u003cb\u003eSection 5: Plant expression\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e16.\u003c\/b\u003e\u003cb\u003e \u003c\/b\u003eMicroalgae as solar-powered protein factories; \u003ci\u003eFranziska Hempel and Uwe G. Maier\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003e17.\u003cb\u003e \u003c\/b\u003eStrategies and methodologies for the co-expression of multiple proteins in plants; \u003ci\u003eAlbert Ferrer, Montserrat Arró, David Manzano and Teresa Altabella\u003c\/i\u003e                \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e18\u003c\/b\u003e\u003cb\u003e.\u003c\/b\u003e Transient expression systems in plants—potentialities and constraints; \u003ci\u003eTomas Canto\u003c\/i\u003e         \u003c\/p\u003e  \u003cb\u003eSection 6: Complex reconstitution\u003c\/b\u003e  \u003cp\u003e\u003cb\u003e19.\u003c\/b\u003e\u003cb\u003e \u003c\/b\u003eComplex reconstitution from individual protein modules; \u003ci\u003eJérome Basquin, Michael Taschner and Esben Lorentzen\u003c\/i\u003e                                                                             \u003c\/p\u003e  \u003cp\u003e\u003cb\u003e20.\u003c\/b\u003e\u003cb\u003e \u003c\/b\u003eStructural reconstruction of protein-protein complexes involved in intracellular signaling; \u003ci\u003eKlára Kirsch, Péter Sok, and Attila Remény\u003c\/i\u003e                                                           \u003c\/p\u003e  \u003cp\u003e\u003cb\u003eSection 7: Biophysical methods to assess protein complexes\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003e21.\u003c\/b\u003e\u003cb\u003e \u003c\/b\u003eThe use of small-angle scattering for the characterization of multi subunit complexes; \u003ci\u003eAdam Round\u003c\/i\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003e22.\u003c\/b\u003e\u003cb\u003e \u003c\/b\u003eApplication of nuclear magnetic resonance and hybrid methods to structure determination of complex systems. \u003ci\u003eFilippo Prischi and Annalisa Pastore\u003c\/i\u003e\u003c\/p\u003e","brand":"Springer International Publishing AG","offers":[{"title":"Default Title","offer_id":51021157106007,"sku":"9783319272146","price":143.99,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0817\/1739\/5799\/files\/9783319272146.jpg?v=1750785320","url":"https:\/\/bookcurl.com\/products\/advanced-technologies-for-protein-complex-production-and-characterization-9783319272146","provider":"Book Curl","version":"1.0","type":"link"}